TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig2813  13099 14725                                                48   7e-05
Contig84498  680 680                                                   37   0.15 

>Contig2813  13099 14725
          Length = 14725

 Score = 48.1 bits (113), Expect = 7e-05
 Identities = 31/92 (33%), Positives = 47/92 (51%)
 Frame = -1

Query: 173  VCHRSELIMPYNAQPGDALVLTKPLGTQLATNAYIWMGEQQSDSWAQLRERFTVADIEQT 232
            + H  ++     A+ GDA++LTK +GT + T A               R   + A    T
Sbjct: 5776 IVHPHKIWKNNTARDGDAIILTKRIGTGICTTA-------------MKRNMASPA----T 5648

Query: 233  YRIALESMSRLNKTGAELMKKYGAHAATDVTG 264
             + A+++MS LNK  AE++ KY  H+ TDVTG
Sbjct: 5647 AQAAIKTMSELNKYAAEVLLKYKVHSCTDVTG 5552



 Score = 42.4 bits (98), Expect = 0.004
 Identities = 19/44 (43%), Positives = 29/44 (65%)
 Frame = -2

Query: 76   LVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQIKLI 119
            ++ +VDFF P++DD +  G IA AN +SDV+A+G      I L+
Sbjct: 6507 II*TVDFFTPVVDDAYTFGAIAAANSLSDVYAMGTLSPPLISLL 6376



 Score = 30.8 bits (68), Expect(2) = 0.013
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = -3

Query: 264  GFGLYGHAENLASHQTADVDFHLDTLPIIKNVREIA 299
            GFGL GH   +A      V  + D +P+I+  +++A
Sbjct: 5324 GFGLAGHMWEMAKSSDLSVHLYFDQIPLIEGTQDLA 5217



 Score = 28.5 bits (62), Expect(2) = 0.013
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = -2

Query: 232  TYRIALESMSRLNKTGAELMKKYGAHAATDVT 263
            T +  +++MS LNK  AE++ KY   +  DVT
Sbjct: 5484 TAQEVIKTMSELNKYAAEVLLKYKVSSGADVT 5389


>Contig84498  680 680
          Length = 680

 Score = 37.0 bits (84), Expect = 0.15
 Identities = 15/31 (48%), Positives = 23/31 (74%)
 Frame = -2

Query: 76  LVQSVDFFYPLIDDPFMLGKIALANVVSDVF 106
           ++ +VDFF P++DD +  G IA AN +SDV+
Sbjct: 94  II*TVDFFTPVVDDAYTFGAIAAANSLSDVY 2


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 120,491,260
Number of Sequences: 297657
Number of extensions: 1611817
Number of successful extensions: 8163
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 7495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8140
length of query: 369
length of database: 106,339,350
effective HSP length: 115
effective length of query: 254
effective length of database: 72,108,795
effective search space: 18315633930
effective search space used: 18315633930
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)