TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplace...    78   2e-41

>gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251335, whole genome shotgun
             sequence
          Length = 70427

 Score = 77.8 bits (190), Expect(3) = 2e-41
 Identities = 46/136 (33%), Positives = 77/136 (56%), Gaps = 2/136 (1%)
 Frame = -3

Query: 1     MFKPESYGLSHDFRLTKFSTLRGUGSKVPQDVLNRLLAGVYGEQLGDKDGKGPNKEEGVG 60
             ++ P  + L   F LT +S L+G G KVPQ  L + L     E+LG       N +    
Sbjct: 41844 LWNPTDHNLEKTFVLTDYSDLKG*GCKVPQADLKKYL-----EKLG-------NAK*ITS 41701

Query: 61    IGLDSSVIALKHDLF--LVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQIKL 118
                D SV  +++     L+ ++DFFYPL++DP+M G+IA  NV+SD+F++G T+ID + +
Sbjct: 41700 EAPDVSVRPVRNTKTKKLISTIDFFYPLVEDPYM*GRIACCNVLSDLFSMGVTDIDTVLM 41521

Query: 119   IVTAPTEFTEQEREVV 134
             ++    +    ++EV+
Sbjct: 41520 VLAVSNKMNA*QKEVI 41473



 Score = 69.3 bits (168), Expect(3) = 2e-41
 Identities = 48/104 (46%), Positives = 58/104 (55%)
 Frame = -1

Query: 250   LMKKYGAHAATDVTGFGLYGHAENLASHQTADVDFHLDTLPIIKNVREIAETLGRGAKLL 309
             LMKKY A+A TDVTGFG+ GH+  LA      VD  ++ LP IK V  I +   R  K  
Sbjct: 41075 LMKKYKANACTDVTGFGIKGHS*FLAEV*KE*VDLIINNLPTIKYVASI-DGKARDFKFK 40899

Query: 310   AGKAVETSGGLLICLPREAAAGFCEEYRRCTKHEAWIVGHVEKG 353
              G + ETSGGLLICL +  A  F  E +     E  +VG V KG
Sbjct: 40898 *GLSPETSGGLLICLEKNKAKEFISEMKD-NGLECNLVGEVVKG 40770



 Score = 62.4 bits (150), Expect(3) = 2e-41
 Identities = 35/83 (42%), Positives = 46/83 (55%)
 Frame = -2

Query: 133   VVVPMVMQGFLEAAKACNAPVQIGSIAENPWCVIGGAASAVCHRSELIMPYNAQPGDALV 192
             +V  +++ GF E A    A V  G    NPW +IGG   +V  R E +MP NA+ GD ++
Sbjct: 41425 IVTSLMI*GFDECA**VGAYVLGG*TIINPWPIIGGVGISVVEREEFLMPNNAKVGDMII 41246

Query: 193   LTKPLGTQLATNAYIWMGEQQSD 215
             LTKPLGT LA N   W  +   D
Sbjct: 41245 LTKPLGT*LAVNLK*WYTKDIKD 41177


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,583,439
Number of Sequences: 2017
Number of extensions: 112472
Number of successful extensions: 185
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 168
Number of HSP's gapped (non-prelim): 20
length of query: 369
length of database: 16,266,656
effective HSP length: 105
effective length of query: 264
effective length of database: 16,054,871
effective search space: 4238485944
effective search space used: 4238485944
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)