TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|70879148|gb|EAN92345.1| hypothetical protein, conserved
[Trypanosoma cruzi]
         (386 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig8624  4696 7507                                                  33   2.3  
Contig6085  7388 7787                                                  32   6.8  

>Contig8624  4696 7507
          Length = 7507

 Score = 33.1 bits (74), Expect = 2.3
 Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
 Frame = +3

Query: 23   IQRLSEQVTTEGERSNS----RHGGVVEAVLELDTFISSYEERDGTQRNGSNFSPEAWRR 78
            ++R++E++   G R N     R+ G  E   E +    +  ER+GT+RNG+       R+
Sbjct: 4386 LRRMAEEINLGGRRRNGSRAERNDGTTEREAERNGTERNGTERNGTERNGTERKRRGGRK 4565

Query: 79   ACDEVREATFQRIR 92
               E R+   +R R
Sbjct: 4566 GRKEKRKRQRKRKR 4607


>Contig6085  7388 7787
          Length = 7787

 Score = 31.6 bits (70), Expect = 6.8
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -1

Query: 1    MRICLVLLSGLPGAGKTTLSLAIQRLSEQVTTEGERSNSRHGG 43
            + +CL  L G+P  G  + S + + L E +   GE+S  R GG
Sbjct: 6272 LSLCL*GLKGVPAFGTNSTSWSCKFLLESIKGRGEKSKGRRGG 6144


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.319    0.133    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 145,184,639
Number of Sequences: 297657
Number of extensions: 2100500
Number of successful extensions: 10418
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 9866
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10401
length of query: 386
length of database: 106,339,350
effective HSP length: 115
effective length of query: 271
effective length of database: 72,108,795
effective search space: 19541483445
effective search space used: 19541483445
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 69 (31.2 bits)