TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|27369676|ref|NP_766078.1| O-phosphoseryl-tRNA(Sec) selenium
transferase [Mus musculus]
         (504 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig19987  1321 1331                                                117   2e-30
Contig8169  4144 7930                                                 119   5e-26
Contig98548  227 227                                                   48   1e-04
Contig5725  8000 12788                                                 33   2.5  
Contig4747  9400 16846                                                 32   7.3  

>Contig19987  1321 1331
          Length = 1331

 Score =  117 bits (292), Expect(2) = 2e-30
 Identities = 61/92 (66%), Positives = 68/92 (73%)
 Frame = -3

Query: 251  VNNAYGLQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNEPFIQDISKMY 310
            VNNAYGLQ       I Q A+ GRIDA +QS DKNFMVPVGGAIIAG +E F+  I+K Y
Sbjct: 1278 VNNAYGLQDHAVCQGITQAAKKGRIDAIIQSTDKNFMVPVGGAIIAGPDEKFMNAIAKNY 1099

Query: 311  PGRASASPSLDVLITLLSLGCSGYRKLLKERK 342
            PGRAS SP LD+ ITL+SLG  GY KLL ERK
Sbjct: 1098 PGRASGSPVLDLFITLISLGRDGYEKLLHERK 1003



 Score = 37.7 bits (86), Expect(2) = 2e-30
 Identities = 15/30 (50%), Positives = 22/30 (73%)
 Frame = -2

Query: 343 EMFVYLSTQLKKLAEAHNERLLQTPHNPIS 372
           EM+ YL  ++  +A+ + ERLL TPHNP+S
Sbjct: 913 EMYNYLKEKMSAIAKKYGERLLLTPHNPVS 824


>Contig8169  4144 7930
          Length = 7930

 Score =  119 bits (297), Expect = 5e-26
 Identities = 84/211 (39%), Positives = 112/211 (53%), Gaps = 48/211 (22%)
 Frame = -3

Query: 40   KCPEDGWDESTLELFLHELAVMDSNNFLGNCGVGEREGRVASALVARRHYR-----FIHG 94
            + PE GWDE T+E  LH +A MDSNNF+ N GVGEREGRVAS +VA+RHYR     F++ 
Sbjct: 3455 RLPESGWDEGTIEYILHTIAGMDSNNFIKNVGVGEREGRVASGIVAKRHYR*TNYLFLNR 3276

Query: 95   IGRSGDISAVQPKAAGS---------SLLNKITNSLV---------------LNVIKL-- 128
               S  + +   K  G+         S  N   + L+               L ++K   
Sbjct: 3275 H*HSN*VRSWCWKEWGNNRTSAQSCWSFPNF*IDQLLSY*CHACCWYLFFSSLFILKFR* 3096

Query: 129  --AGVHSVASCFVVPMATGMSLTLCFLTLRHKRP--------------KAKYII-WPRID 171
               G+   A+  V P+ATGM+LTL  LTL+ +                K +Y++ WPRID
Sbjct: 3095 FNLGLRIPAAIVV-PLATGMALTLTLLTLKQQHDEKQLSDAQIAINDIKNQYVVFWPRID 2919

Query: 172  QKSCFKSMVTAGFEPVVIENVLEGDELRTDL 202
            QKSC KS+ TAGF P+ I   L+GDELRT +
Sbjct: 2918 QKSCLKSIHTAGFRPIPIPVKLDGDELRTGM 2826



 Score = 76.3 bits (186), Expect = 3e-13
 Identities = 39/88 (44%), Positives = 56/88 (63%), Gaps = 1/88 (1%)
 Frame = -2

Query: 383 HHDKAVTQLGSMLFTRQVSGARAVPLGNVQTVSGHTFRGFMSHADNYPCA-YLNAAAAIG 441
           H  K  T +GSMLF R VSG R +  G  ++V+G  F GF +H D YP + YL AAAA+G
Sbjct: 474 HTKKDATSVGSMLFARNVSGVRVISGGERKSVAGIWFDGFGAHTDKYPFSPYLTAAAAVG 295

Query: 442 MKMQDVDLFIKRLDKCLNIVRKEQTRAS 469
           M  ++VD+F +R+DK L+ ++     +S
Sbjct: 294 MTKKEVDIFAERIDKVLSSLQPSSATSS 211


>Contig98548  227 227
          Length = 227

 Score = 48.1 bits (113), Expect = 1e-04
 Identities = 21/59 (35%), Positives = 38/59 (64%), Gaps = 3/59 (5%)
 Frame = +1

Query: 198 LRTDLKAVEAKIQELG---PEHILCLHSTTACFAPRVPDRLEELAVICANYDIPHVVNN 253
           + +DL A+E  +++     P   + + +TT+CFAPR PDR++++A +C  Y + HV N+
Sbjct: 22  ITSDLAALEEAVRKHTTEFPHACVVVMTTTSCFAPRAPDRVDDVATLCQKYGVAHVGNS 198


>Contig5725  8000 12788
          Length = 12788

 Score = 33.5 bits (75), Expect = 2.5
 Identities = 23/71 (32%), Positives = 35/71 (49%)
 Frame = +1

Query: 108  AAGSSLLNKITNSLVLNVIKLAGVHSVASCFVVPMATGMSLTLCFLTLRHKRPKAKYIIW 167
            +AG SL   I+N  + N I+    HS+  C V  +   MS  +     R+   KA+Y +W
Sbjct: 3997 SAGMSLEKSISNK*ITN-IE*RSKHSITYCVVSSIGAKMSF*IH--RSRYINIKARYFVW 4167

Query: 168  PRIDQKSCFKS 178
              I  KS F++
Sbjct: 4168 LHIKSKSLFRN 4200


>Contig4747  9400 16846
          Length = 16846

 Score = 32.0 bits (71), Expect = 7.3
 Identities = 14/40 (35%), Positives = 24/40 (60%)
 Frame = +2

Query: 307  SKMYPGRASASPSLDVLITLLSLGCSGYRKLLKERKEMFV 346
            S +YP  +S SPS  +L+  +S G  G++K  + R  +F+
Sbjct: 2960 SPLYPPPSSPSPSYTLLLPHVSFGYLGFQKFAQNRNRLFL 3079


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 178,659,769
Number of Sequences: 297657
Number of extensions: 2498629
Number of successful extensions: 11125
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 10517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11122
length of query: 504
length of database: 106,339,350
effective HSP length: 118
effective length of query: 386
effective length of database: 71,215,824
effective search space: 27489308064
effective search space used: 27489308064
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 70 (31.6 bits)