TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|27369676|ref|NP_766078.1| O-phosphoseryl-tRNA(Sec) selenium
transferase [Mus musculus]
         (504 letters)

Database: F.cylindrus/genome.fa 
           271 sequences; 80,540,407 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_2                                                            177   5e-44

>scaffold_2
          Length = 3822992

 Score =  177 bits (448), Expect = 5e-44
 Identities = 96/215 (44%), Positives = 138/215 (64%), Gaps = 8/215 (3%)
 Frame = +3

Query: 85     ARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLNVI----KLAGVHSVASCFVV 140
              A RHY   HG+GRSGDI+  QPKAAGSS L K+   L L+ +    KL      A   ++
Sbjct: 704229 AFRHYGLSHGMGRSGDITEPQPKAAGSSALVKLALLLTLDALRRGSKLDAKGPAAYGILL 704408

Query: 141    PMATGMSLTLCFLTLRHKRP----KAKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLEGD 196
              P+ TGMS++L   ++R K      K   ++W RIDQKSCFK++ +AG   VV+   +EGD
Sbjct: 704409 PLCTGMSISLLLASIRSKEENSETKKDIVLWSRIDQKSCFKAIHSAGLICVVVPTKIEGD 704588

Query: 197    ELRTDLKAVEAKIQELGPEHILCLHSTTACFAPRVPDRLEELAVICANYDIPHVVNNAYG 256
              E+ TD+ A+EA + +     +L + +T++CF PRVPD ++++A +C   ++ H++NNAYG
Sbjct: 704589 EVITDIDAMEAALSK-HEGRVLAVITTSSCFTPRVPDAVDKVAKLCKVSNVYHIINNAYG 704765

Query: 257    LQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVG 291
              LQ +K   LI +   VGR+DA V S DKNF+VPVG
Sbjct: 704766 LQCAKTSKLINRACVVGRVDAVVCSTDKNFLVPVG 704870



 Score =  140 bits (354), Expect = 4e-33
 Identities = 79/200 (39%), Positives = 122/200 (61%), Gaps = 14/200 (7%)
 Frame = +2

Query: 287    MVPVGGAIIAGFNEPFIQDISKMYPGRASASPSLDVLITLLSLGCSGYRKLLKERKEMFV 346
              +V  GGAII   ++  I ++ K+Y GRAS+SP +D+ ITLLS+G +GY++LLKER  +  
Sbjct: 705056 VVVSGGAIIISPSKNIIGEVGKVYAGRASSSPIVDLFITLLSMGMNGYKRLLKERTSVLE 705235

Query: 347    YLSTQLKKLAEAHNERLLQTPHNPISLAMTL------KTIDGHHD-------KAVTQLGS 393
                  +L+++AE H ER+L  P+N +S  +TL      K +D   +       K +T  G+
Sbjct: 705236 TFPERLREVAEKHGERILVCPNNTVSFGLTLDGLARPKEVDELDENYTQSTAKEITSFGA 705415

Query: 394    MLFTRQVSGARAVPLGNVQTVSGHTFRGFMSHADNYPCAYLNAAAAIGMKMQDVDLFIKR 453
              MLF+R VSG R VP   ++++ G  F GF S  + YP AY+ AA AIG+ + +++ F  R
Sbjct: 705416 MLFSRCVSGTRVVPRSQLKSIGGQDFIGFGSSTNEYPHAYMTAACAIGVSIFEIEEFYVR 705595

Query: 454    LDKCL-NIVRKEQTRASVVS 472
              LDK L    RK++ +A  ++
Sbjct: 705596 LDKTLTEFKRKKKKKAQKIA 705655



 Score = 43.5 bits (101), Expect = 0.001
 Identities = 25/65 (38%), Positives = 37/65 (56%), Gaps = 12/65 (18%)
 Frame = +1

Query: 40     KCPEDGWDESTLELFLHELAVMDSNN----FLGN--------CGVGEREGRVASALVARR 87
              + PE GW +  ++  L   + +D+NN      GN        CGVGEREGRV S+LV++R
Sbjct: 703774 RLPEYGWSDVQIQSLLFLFSTLDTNNKTLATCGNNEEDDTRWCGVGEREGRVFSSLVSQR 703953

Query: 88     HYRFI 92
                +F+
Sbjct: 703954 *VQFM 703968


  Database: F.cylindrus/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 80,540,407
  Number of sequences in database:  271
  
Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,994,464
Number of Sequences: 271
Number of extensions: 612577
Number of successful extensions: 2369
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2266
Number of HSP's gapped (non-prelim): 220
length of query: 504
length of database: 26,846,802
effective HSP length: 111
effective length of query: 393
effective length of database: 26,816,721
effective search space: 10538971353
effective search space used: 10538971353
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 66 (30.0 bits)