TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|27369676|ref|NP_766078.1| O-phosphoseryl-tRNA(Sec) selenium
transferase [Mus musculus]
         (504 letters)

Database: D.fasciculatum/genome.fa 
           25 sequences; 31,019,208 total letters

Searching.........................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|328872098|gb|GL883010.1| Dictyostelium fasciculatum unplaced ...   427   e-121
gi|328870851|gb|GL883015.1| Dictyostelium fasciculatum unplaced ...    32   1.1  

>gi|328872098|gb|GL883010.1| Dictyostelium fasciculatum unplaced genomic
               scaffold Scf_Dfasci-F-a-25c12.r2-5, whole genome shotgun
               sequence
          Length = 4166812

 Score =  427 bits (1097), Expect(2) = e-121
 Identities = 222/444 (50%), Positives = 303/444 (68%), Gaps = 25/444 (5%)
 Frame = +3

Query: 40      KCPEDGWDESTLELFLHELAVMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSG 99
               + P+ GWD+  +  FL+++A MDSNN++ N GVGEREGR+ S +V  RH+   HGIGRSG
Sbjct: 3466932 RLPDKGWDDQFIRWFLNDIAKMDSNNYVENAGVGEREGRIHSRIVQERHFGLAHGIGRSG 3467111

Query: 100     DISAVQPKAAGSSLLNKITNSLVLNVIKLAGVHSVASCFVVPMATGMSLTLCFLTL-RHK 158
               DIS  QPKAAGSSL+ ++ + LV++ + +AG++S+  C V+PMATGM++ L  +T  +H 
Sbjct: 3467112 DISEQQPKAAGSSLVQRLCSYLVMDSMSVAGLNSIGECLVLPMATGMTIALTLMTFTKHC 3467291

Query: 159     RPK-AKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLEGDELRTDLKAVEAKIQELGPEHI 217
               RP  AKY+IWPRIDQKSC K++ TAG  PVVIENVL+GD + TDL+A+E KI ELGPE++
Sbjct: 3467292 RPPTAKYVIWPRIDQKSCLKAIATAGLVPVVIENVLQGDVITTDLEAIENKIIELGPENV 3467471

Query: 218     LCLHSTTACFAPRVPDRLEELAVICANYDIPHVVNNAYGLQSSKCMHLIQ---QGARVGR 274
               +C++STT+CFAPRVPDR+ E++ +C  Y++ H++NNAYGLQ SK +H I    Q  +  R
Sbjct: 3467472 VCVYSTTSCFAPRVPDRIIEISELCYKYNVGHIINNAYGLQCSKIVHAINESCQSDKQRR 3467651

Query: 275     IDAFVQSLDKNFMVPVGGAIIAGFNEP-----------------FIQDISKMYPGRASAS 317
               +DA +QS DKN+MVPVGGAII    +                   I  ISK YPGRA+ S
Sbjct: 3467652 VDAIIQSTDKNYMVPVGGAIITSPKKEKQQHKQKEKGEEEQQVNLIDLISKNYPGRANGS 3467831

Query: 318     PSLDVLITLLSLGCSGYRKLLKERKEMFVYLSTQLKKLA-EAHNERLLQTPHNPISLAMT 376
               P LD+ ITLLS+G  GY++LL ERK++  Y   QL  L+ +  N RLL TP N ISL + 
Sbjct: 3467832 PILDLFITLLSMGRQGYKELLDERKQLLTYFRDQLASLSLQFPNCRLLDTPDNRISLGLA 3468011

Query: 377     LKTIDGHHDK-AVTQLGSMLFTRQVSGARAVPL-GNVQTVSGHTFRGFMSHADNYPCAYL 434
               L    G   K +++ +GS LF+R  SGAR + L G+ ++V+G  F  + SH +NY  +YL
Sbjct: 3468012 LAVASGDQQKLSISMIGSKLFSRSCSGARVIELPGSKKSVAGLDFESYGSHINNYHSSYL 3468191

Query: 435     NAAAAIGMKMQDVDLFIKRLDKCL 458
                 A AIG+   D+D F+KRL K L
Sbjct: 3468192 TVACAIGITKDDIDTFMKRLKKIL 3468263



 Score = 29.3 bits (64), Expect(2) = e-121
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +2

Query: 1       MNPESFAAGERRVSPAYVRQGCEARRAHEHLIRLLLEQ 38
               MN ++    +  ++P+Y+ Q  +A   + HLI +LL Q
Sbjct: 3466718 MNQKNLEICKSLINPSYIEQSSQAINQYNHLINVLLTQ 3466831


>gi|328870851|gb|GL883015.1| Dictyostelium fasciculatum unplaced genomic
              scaffold Scf_Dfasci-G-b-126g04.f1-5, whole genome shotgun
              sequence
          Length = 499581

 Score = 32.0 bits (71), Expect = 1.1
 Identities = 24/95 (25%), Positives = 46/95 (48%), Gaps = 1/95 (1%)
 Frame = +1

Query: 101    ISAVQPKAAGSSLLNKITNSLVLNVIK-LAGVHSVASCFVVPMATGMSLTLCFLTLRHKR 159
              I++++ +  G   +NKI   ++LN+I  +  +  +  C+VV   + + L L  +T  H  
Sbjct: 144517 ITSIKQQGEGED*INKIPTIIILNIINHVEDIIDLVCCYVVKDYSTLLLLLTIITNYHLN 144696

Query: 160    PKAKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLE 194
                       + D K   KS V+  F P ++++ LE
Sbjct: 144697 KSNSSTQMYKADSKK--KSGVSL-FRPSILDHSLE 144792


  Database: D.fasciculatum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 31,019,208
  Number of sequences in database:  25
  
Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,967,527
Number of Sequences: 25
Number of extensions: 222246
Number of successful extensions: 802
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 751
Number of HSP's gapped (non-prelim): 51
length of query: 504
length of database: 10,339,736
effective HSP length: 104
effective length of query: 400
effective length of database: 10,337,136
effective search space: 4134854400
effective search space used: 4134854400
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)