TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|255071835|ref|XP_002499592.1| O-phosphoseryl-tRNA selenium
transferase [Micromonas sp. RCC299]
         (498 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig8169  4144 7930                                                  67   7e-34
Contig19987  1321 1331                                                110   1e-27
Contig98548  227 227                                                   43   0.004

>Contig8169  4144 7930
          Length = 7930

 Score = 67.4 bits (163), Expect(3) = 7e-34
 Identities = 31/51 (60%), Positives = 39/51 (76%)
 Frame = -3

Query: 40   RRLPKDGWDDHSIESFLSEAAMMDSNNFLDNVGVGEREARVYSPLVARRHW 90
            +RLP+ GWD+ +IE  L   A MDSNNF+ NVGVGERE RV S +VA+RH+
Sbjct: 3458 KRLPESGWDEGTIEYILHTIAGMDSNNFIKNVGVGEREGRVASGIVAKRHY 3306



 Score = 63.9 bits (154), Expect(3) = 7e-34
 Identities = 29/47 (61%), Positives = 37/47 (78%)
 Frame = -1

Query: 93   AHGIGRSGDVAAEQPKAAGSSLLNKLTNLLVADAMRIAGLEDMGPAL 139
            AHG+GRSG++   QPKAAG+SL++KLTN LV DAMR+AG+    P L
Sbjct: 3253 AHGVGRSGEITEPQPKAAGASLISKLTNYLVIDAMRVAGIYSFPPYL 3113



 Score = 58.5 bits (140), Expect = 7e-08
 Identities = 32/72 (44%), Positives = 44/72 (61%), Gaps = 1/72 (1%)
 Frame = -2

Query: 392 KDVSFFGSMLFSRCVSGTRVVAPGKRQXXXXXXXXXXXASHDAYPV-PYFTAAAALGTTR 450
           KD +  GSMLF+R VSG RV++ G+R+           A  D YP  PY TAAAA+G T+
Sbjct: 465 KDATSVGSMLFARNVSGVRVISGGERKSVAGIWFDGFGAHTDKYPFSPYLTAAAAVGMTK 286

Query: 451 EDVDRFCAQLRK 462
           ++VD F  ++ K
Sbjct: 285 KEVDIFAERIDK 250



 Score = 55.1 bits (131), Expect(3) = 7e-34
 Identities = 31/81 (38%), Positives = 45/81 (55%), Gaps = 14/81 (17%)
 Frame = -3

Query: 138  ALVLPLATGMTLTLVLLAVKSKRP--------------KTATKVVWCRLDQKTCVKAVLS 183
            A+V+PLATGM LTL LL +K +                K    V W R+DQK+C+K++ +
Sbjct: 3068 AIVVPLATGMALTLTLLTLKQQHDEKQLSDAQIAINDIKNQYVVFWPRIDQKSCLKSIHT 2889

Query: 184  AGLELVVVSPKLVGDQLCTDL 204
            AG   + +  KL GD+L T +
Sbjct: 2888 AGFRPIPIPVKLDGDELRTGM 2826



 Score = 32.7 bits (73), Expect = 4.2
 Identities = 15/33 (45%), Positives = 23/33 (69%)
 Frame = -2

Query: 7    YALAEGLVSKAYINQGSQATARRSKLVTSLLSE 39
            Y LA+ LV K+Y+   SQA  +R  L+T+LL++
Sbjct: 3942 YELAQSLVPKSYLTLSSQAHHQRENLLTNLLAQ 3844


>Contig19987  1321 1331
          Length = 1331

 Score =  110 bits (276), Expect(2) = 1e-27
 Identities = 51/93 (54%), Positives = 75/93 (80%)
 Frame = -3

Query: 253  VNNAYGVQATALCKEVTKAWRRGRVDAVVQSTDKNFMVPVGGAVVCSGKGNTEIVDAVRK 312
            VNNAYG+Q  A+C+ +T+A ++GR+DA++QSTDKNFMVPVGGA++     + + ++A+ K
Sbjct: 1278 VNNAYGLQDHAVCQGITQAAKKGRIDAIIQSTDKNFMVPVGGAIIAG--PDEKFMNAIAK 1105

Query: 313  SYPGRASISPVLDLLITLLSMGSHGWQRCLEDR 345
            +YPGRAS SPVLDL ITL+S+G  G+++ L +R
Sbjct: 1104 NYPGRASGSPVLDLFITLISLGRDGYEKLLHER 1006



 Score = 34.3 bits (77), Expect(2) = 1e-27
 Identities = 13/30 (43%), Positives = 21/30 (70%)
 Frame = -2

Query: 347 DVYGYMRTKLGECVGEFGERLLDTPGNPIS 376
           ++Y Y++ K+     ++GERLL TP NP+S
Sbjct: 913 EMYNYLKEKMSAIAKKYGERLLLTPHNPVS 824


>Contig98548  227 227
          Length = 227

 Score = 42.7 bits (99), Expect = 0.004
 Identities = 19/34 (55%), Positives = 24/34 (70%)
 Frame = +1

Query: 222 VVTSTSCFAPRAGDACSDVAKMCAELDVGHVVNN 255
           V+T+TSCFAPRA D   DVA +C +  V HV N+
Sbjct: 97  VMTTTSCFAPRAPDRVDDVATLCQKYGVAHVGNS 198


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.319    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 160,124,143
Number of Sequences: 297657
Number of extensions: 2115349
Number of successful extensions: 7165
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 6888
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7160
length of query: 498
length of database: 106,339,350
effective HSP length: 118
effective length of query: 380
effective length of database: 71,215,824
effective search space: 27062013120
effective search space used: 27062013120
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 70 (31.6 bits)