TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|255071835|ref|XP_002499592.1| O-phosphoseryl-tRNA selenium
transferase [Micromonas sp. RCC299]
         (498 letters)

Database: F.cylindrus/genome.fa 
           271 sequences; 80,540,407 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_2                                                            188   2e-47
scaffold_244                                                           30   8.0  

>scaffold_2
          Length = 3822992

 Score =  188 bits (478), Expect = 2e-47
 Identities = 105/215 (48%), Positives = 140/215 (65%), Gaps = 7/215 (3%)
 Frame = +3

Query: 86     ARRHWNLAHGIGRSGDVAAEQPKAAGSSLLNKLTNLLVADAMRIAG-LEDMGPA---LVL 141
              A RH+ L+HG+GRSGD+   QPKAAGSS L KL  LL  DA+R    L+  GPA   ++L
Sbjct: 704229 AFRHYGLSHGMGRSGDITEPQPKAAGSSALVKLALLLTLDALRRGSKLDAKGPAAYGILL 704408

Query: 142    PLATGMTLTLVLLAVKSKRPKTATK---VVWCRLDQKTCVKAVLSAGLELVVVSPKLVGD 198
              PL TGM+++L+L +++SK   + TK   V+W R+DQK+C KA+ SAGL  VVV  K+ GD
Sbjct: 704409 PLCTGMSISLLLASIRSKEENSETKKDIVLWSRIDQKSCFKAIHSAGLICVVVPTKIEGD 704588

Query: 199    QLCTDLDXXXXXXXXXXXXXXCCVVTSTSCFAPRAGDACSDVAKMCAELDVGHVVNNAYG 258
              ++ TD+D                V+T++SCF PR  DA   VAK+C   +V H++NNAYG
Sbjct: 704589 EVITDIDAMEAALSKHEGRVLA-VITTSSCFTPRVPDAVDKVAKLCKVSNVYHIINNAYG 704765

Query: 259    VQATALCKEVTKAWRRGRVDAVVQSTDKNFMVPVG 293
              +Q     K + +A   GRVDAVV STDKNF+VPVG
Sbjct: 704766 LQCAKTSKLINRACVVGRVDAVVCSTDKNFLVPVG 704870



 Score =  117 bits (293), Expect = 5e-26
 Identities = 71/185 (38%), Positives = 104/185 (56%), Gaps = 11/185 (5%)
 Frame = +2

Query: 289    MVPVGGAVVCSGKGNTEIVDAVRKSYPGRASISPVLDLLITLLSMGSHGWQRCLEDRGDV 348
              +V  GGA++ S   N  I+  V K Y GRAS SP++DL ITLLSMG +G++R L++R  V
Sbjct: 705056 VVVSGGAIIISPSKN--IIGEVGKVYAGRASSSPIVDLFITLLSMGMNGYKRLLKERTSV 705229

Query: 349    YGYMRTKLGECVGEFGERLLDTPGNPISIGVSLETHAKLSE-----------DGKDVSFF 397
                    +L E   + GER+L  P N +S G++L+  A+  E             K+++ F
Sbjct: 705230 LETFPERLREVAEKHGERILVCPNNTVSFGLTLDGLARPKEVDELDENYTQSTAKEITSF 705409

Query: 398    GSMLFSRCVSGTRVVAPGKRQXXXXXXXXXXXASHDAYPVPYFTAAAALGTTREDVDRFC 457
              G+MLFSRCVSGTRVV   + +           +S + YP  Y TAA A+G +  +++ F 
Sbjct: 705410 GAMLFSRCVSGTRVVPRSQLKSIGGQDFIGFGSSTNEYPHAYMTAACAIGVSIFEIEEFY 705589

Query: 458    AQLRK 462
               +L K
Sbjct: 705590 VRLDK 705604



 Score = 45.4 bits (106), Expect = 2e-04
 Identities = 30/87 (34%), Positives = 43/87 (49%), Gaps = 12/87 (13%)
 Frame = +1

Query: 14     VSKAYINQGSQATARRSKLVTSLLSERRLPKDGWDDHSIESFLSEAAMMDSNNFL----- 68
              + K +   G       +K   SL   +RLP+ GW D  I+S L   + +D+NN       
Sbjct: 703693 IPKTHATVGLTNLIASTKQYNSLFIHKRLPEYGWSDVQIQSLLFLFSTLDTNNKTLATCG 703872

Query: 69     ----DNV---GVGEREARVYSPLVARR 88
                  D+    GVGERE RV+S LV++R
Sbjct: 703873 NNEEDDTRWCGVGEREGRVFSSLVSQR 703953


>scaffold_244
          Length = 3618

 Score = 30.4 bits (67), Expect = 8.0
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -1

Query: 220  CCVVTSTSCFAPRAGDACSDVAKMCAELDVGHVVNNA 256
            CC  TSTSC +  +   CSD +  C      + V+N+
Sbjct: 1155 CCSDTSTSCCSDTSTSCCSDTSTSCCSDTSTNTVSNS 1045


  Database: F.cylindrus/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 80,540,407
  Number of sequences in database:  271
  
Lambda     K      H
   0.319    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,511,978
Number of Sequences: 271
Number of extensions: 493474
Number of successful extensions: 1827
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1710
Number of HSP's gapped (non-prelim): 326
length of query: 498
length of database: 26,846,802
effective HSP length: 111
effective length of query: 387
effective length of database: 26,816,721
effective search space: 10378071027
effective search space used: 10378071027
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)