TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig19987  1321 1331                                                126   2e-28
Contig8169  4144 7930                                                  70   4e-25
Contig98548  227 227                                                   51   1e-05
Contig17736  1688 1688                                                 33   4.0  

>Contig19987  1321 1331
          Length = 1331

 Score =  126 bits (317), Expect = 2e-28
 Identities = 58/92 (63%), Positives = 76/92 (82%)
 Frame = -3

Query: 254  INNAYGLQCSKILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNY 313
            +NNAYGLQ   +   I+QA K GR+DA IQSTDKNFMVPVGGAII+GP+ +F++ I++NY
Sbjct: 1278 VNNAYGLQDHAVCQGITQAAKKGRIDAIIQSTDKNFMVPVGGAIIAGPDEKFMNAIAKNY 1099

Query: 314  PGRANSSPILDVFITLLSMGKQGWLNLLKERK 345
            PGRA+ SP+LD+FITL+S+G+ G+  LL ERK
Sbjct: 1098 PGRASGSPVLDLFITLISLGRDGYEKLLHERK 1003


>Contig8169  4144 7930
          Length = 7930

 Score = 70.5 bits (171), Expect(2) = 4e-25
 Identities = 32/51 (62%), Positives = 42/51 (82%)
 Frame = -3

Query: 39   KKLPNIGYNDKIIELILNEISLMDSNNFIENIGVGEREGRIYSGLVEKRHY 89
            K+LP  G+++  IE IL+ I+ MDSNNFI+N+GVGEREGR+ SG+V KRHY
Sbjct: 3458 KRLPESGWDEGTIEYILHTIAGMDSNNFIKNVGVGEREGRVASGIVAKRHY 3306



 Score = 66.2 bits (160), Expect(2) = 4e-25
 Identities = 30/41 (73%), Positives = 38/41 (92%)
 Frame = -1

Query: 91   FAHGIGRSGDITEQQPKAAGSSLIQKLTHSLVLDAMKLAGL 131
            FAHG+GRSG+ITE QPKAAG+SLI KLT+ LV+DAM++AG+
Sbjct: 3256 FAHGVGRSGEITEPQPKAAGASLISKLTNYLVIDAMRVAGI 3134



 Score = 60.5 bits (145), Expect = 2e-08
 Identities = 31/72 (43%), Positives = 46/72 (63%), Gaps = 1/72 (1%)
 Frame = -2

Query: 407 IGSKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNYSTS-YLTVACAIGITKLEI 465
           +GS LF+R+ SG RVI     K   + G+ F+ +G+H D Y  S YLT A A+G+TK E+
Sbjct: 450 VGSMLFARNVSGVRVISGGERKS--VAGIWFDGFGAHTDKYPFSPYLTAAAAVGMTKKEV 277

Query: 466 DTFIQRLSKLFN 477
           D F +R+ K+ +
Sbjct: 276 DIFAERIDKVLS 241



 Score = 58.9 bits (141), Expect = 5e-08
 Identities = 28/42 (66%), Positives = 32/42 (76%)
 Frame = -3

Query: 164  NKRYVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMIRTDL 205
            N+  V WPRIDQKSCLKSI TAG  PI IP +LDGD +RT +
Sbjct: 2951 NQYVVFWPRIDQKSCLKSIHTAGFRPIPIPVKLDGDELRTGM 2826


>Contig98548  227 227
          Length = 227

 Score = 50.8 bits (120), Expect = 1e-05
 Identities = 20/59 (33%), Positives = 40/59 (67%), Gaps = 3/59 (5%)
 Frame = +1

Query: 201 IRTDLVAIEDKIKELGVD---NILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINN 256
           I +DL A+E+ +++   +     + V +TTSCFAPR PD++ +++ +C++Y + H+ N+
Sbjct: 22  ITSDLAALEEAVRKHTTEFPHACVVVMTTTSCFAPRAPDRVDDVATLCQKYGVAHVGNS 198


>Contig17736  1688 1688
          Length = 1688

 Score = 32.7 bits (73), Expect = 4.0
 Identities = 18/76 (23%), Positives = 40/76 (52%)
 Frame = +1

Query: 12  LIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISLMDSNNFIENIG 71
           +IKG  I+  ++  + F   L+ + ++ ++PN+    K I+ +L   +++  +   E   
Sbjct: 106 IIKGEVINTEVR*RA-FESYLQDVPVYCRIPNVASLFKAIQALLQLTNMVGVSAIFETNR 282

Query: 72  VGEREGRIYSGLVEKR 87
           +   +GR+Y  +VE R
Sbjct: 283 LSHVDGRVYVSIVEVR 330


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 148,956,654
Number of Sequences: 297657
Number of extensions: 1898261
Number of successful extensions: 7036
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 6803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7034
length of query: 479
length of database: 106,339,350
effective HSP length: 117
effective length of query: 362
effective length of database: 71,513,481
effective search space: 25887880122
effective search space used: 25887880122
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 70 (31.6 bits)