TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: L.donovani_BPK282A1/genome.fa 
           36 sequences; 32,444,998 total letters

Searching....................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

contig_09                                                             177   2e-44
contig_25                                                              29   9.1  

>contig_09
          Length = 588727

 Score =  177 bits (448), Expect = 2e-44
 Identities = 118/378 (31%), Positives = 171/378 (45%), Gaps = 67/378 (17%)
 Frame = -2

Query: 166    RYVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMIRTDLVAIEDKIKEL---------- 215
              RYV+WPRIDQK+ LK I  AGL+P+ +  +    + R+    +      L          
Sbjct: 381360 RYVIWPRIDQKTALKCIDAAGLVPVPVQLRPAVPLARSAAPCVSTNRDSLDRGQDSIGSP 381181

Query: 216    --------------------------GVDNILCVFSTTSCFAPRVPDKIIEISEICKRYN 249
                                        G   ++CV STTSCFAPR+PD  + I++ CK+  
Sbjct: 381180 STPTSSSSLFLECHVDDVAAAVNAVGGPSQVVCVLSTTSCFAPRLPDNTVAIAQYCKKAG 381001

Query: 250    IGHIINNAYGLQCSKILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQI 309
              I +++NNAYG+Q  +I+  +  A +LGRVD  +QS DKNF+VPVGG+II   + E    +
Sbjct: 381000 IPYVVNNAYGVQSRRIMTRLDAAQRLGRVDFVVQSGDKNFLVPVGGSIICSGDKERCKAV 380821

Query: 310    SRNYPGRANSSPILDVFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFALENNEKLL-- 367
              +  Y GRA+ SPI+D+FIT LS+G++G   L  +R +       QL  FA E  E LL  
Sbjct: 380820 AALYAGRASMSPIVDLFITALSLGRRGMQTLWSDRYKCRARLIRQLRVFARERREVLLVD 380641

Query: 368    --------------------NTINENKISFAXXXXXXXXXXXXXXXXXXXXXXXXXXXM- 406
                                    +  N IS A                           + 
Sbjct: 380640 DSDDDKADEDTVGGSQRTSNAVVPRNDISVAVTMRAYGLPAAEASSSGAQLGSEQAGRVT 380461

Query: 407    -------IGSKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNY-STSYLTVACAI 458
                     +G++LF  + +G RVI    +    I G  F NYG H D       L +AC I
Sbjct: 380460 NWAAARALGAQLFRSAVTGPRVITPAPSTPTTIAGCTFRNYGMHQDREPPCPLLVIACGI 380281

Query: 459    GITKLEIDTFIQRLSKLF 476
              G+++ E+D  + RL  L+
Sbjct: 380280 GMSESEVDALMARLRDLW 380227



 Score =  115 bits (288), Expect = 7e-26
 Identities = 55/130 (42%), Positives = 85/130 (65%)
 Frame = -2

Query: 1      MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
              M+ ++L+  +  +   YI+   +      +++ ++L  +  P+ G  D  IELIL ++SL
Sbjct: 381999 MDDRSLKLAEDFVSARYIEAGRESLRATARIMRSILAQRCCPDEGLTDAAIELILRQLSL 381820

Query: 61     MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
              MD+NN   ++G GEREGR+ S LV  RH+   HGIGRSGD+  +QPKAAGSSL+ K+T+ 
Sbjct: 381819 MDTNNLAHHVGGGEREGRVVSALVRMRHFHLTHGIGRSGDLFSEQPKAAGSSLLYKITNV 381640

Query: 121    LVLDAMKLAG 130
              L+LD ++ AG
Sbjct: 381639 LMLDLIRQAG 381610


>contig_25
          Length = 897883

 Score = 28.9 bits (63), Expect = 9.1
 Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
 Frame = -2

Query: 68   ENIGVGEREGRIYSGL---VEKRHYGFAHGIGRSGDITEQQPKAAGSSLI 114
            E++  G R+  ++  +   VEK+  G A G GR GD+ +  P+ A SSLI
Sbjct: 8595 ESLRSGRRDDAVHPAVCAYVEKQ--GGA*GRGRGGDLQQAAPRTALSSLI 8452


  Database: L.donovani_BPK282A1/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 32,444,998
  Number of sequences in database:  36
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,876,943
Number of Sequences: 36
Number of extensions: 171638
Number of successful extensions: 644
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 636
Number of HSP's gapped (non-prelim): 31
length of query: 479
length of database: 10,814,999
effective HSP length: 104
effective length of query: 375
effective length of database: 10,811,255
effective search space: 4054220625
effective search space used: 4054220625
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)