TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: F.cylindrus/genome.fa 
           271 sequences; 80,540,407 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_2                                                            187   5e-47
scaffold_15                                                            31   5.9  
scaffold_21                                                            30   7.7  
scaffold_1                                                             30   10.0 

>scaffold_2
          Length = 3822992

 Score =  187 bits (474), Expect = 5e-47
 Identities = 101/213 (47%), Positives = 137/213 (64%), Gaps = 5/213 (2%)
 Frame = +3

Query: 87     RHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHSLVLDAM----KLAGLEQSSLSNCXXX 142
              RHYG +HG+GRSGDITE QPKAAGSS + KL   L LDA+    KL     ++       
Sbjct: 704235 RHYGLSHGMGRSGDITEPQPKAAGSSALVKLALLLTLDALRRGSKLDAKGPAAYGILLPL 704414

Query: 143    XXXXXXXXXXXXXXXKSINANNKR-YVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMI 201
                             K  N+  K+  VLW RIDQKSC K+I +AGLI +V+P +++GD +
Sbjct: 704415 CTGMSISLLLASIRSKEENSETKKDIVLWSRIDQKSCFKAIHSAGLICVVVPTKIEGDEV 704594

Query: 202    RTDLVAIEDKIKELGVDNILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINNAYGLQ 261
               TD+ A+E  + +     +L V +T+SCF PRVPD + +++++CK  N+ HIINNAYGLQ
Sbjct: 704595 ITDIDAMEAALSK-HEGRVLAVITTSSCFTPRVPDAVDKVAKLCKVSNVYHIINNAYGLQ 704771

Query: 262    CSKILHNISQACKLGRVDAFIQSTDKNFMVPVG 294
              C+K    I++AC +GRVDA + STDKNF+VPVG
Sbjct: 704772 CAKTSKLINRACVVGRVDAVVCSTDKNFLVPVG 704870



 Score =  113 bits (283), Expect = 7e-25
 Identities = 72/186 (38%), Positives = 100/186 (53%), Gaps = 1/186 (0%)
 Frame = +2

Query: 290    MVPVGGAIISGPNSEFIDQISRNYPGRANSSPILDVFITLLSMGKQGWLNLLKERKELLI 349
              +V  GGAII  P+   I ++ + Y GRA+SSPI+D+FITLLSMG  G+  LLKER  +L 
Sbjct: 705056 VVVSGGAIIISPSKNIIGEVGKVYAGRASSSPIVDLFITLLSMGMNGYKRLLKERTSVLE 705235

Query: 350    YFNEQLSKFALENNEKLLNTINENKISFA-XXXXXXXXXXXXXXXXXXXXXXXXXXXMIG 408
               F E+L + A ++ E++L   N N +SF                               G
Sbjct: 705236 TFPERLREVAEKHGERILVCPN-NTVSFGLTLDGLARPKEVDELDENYTQSTAKEITSFG 705412

Query: 409    SKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNYSTSYLTVACAIGITKLEIDTF 468
              + LFSR  SG+RV+     K   IGG +F  +GS  + Y  +Y+T ACAIG++  EI+ F
Sbjct: 705413 AMLFSRCVSGTRVVPRSQLKS--IGGQDFIGFGSSTNEYPHAYMTAACAIGVSIFEIEEF 705586

Query: 469    IQRLSK 474
                RL K
Sbjct: 705587 YVRLDK 705604



 Score = 48.1 bits (113), Expect = 4e-05
 Identities = 30/78 (38%), Positives = 42/78 (53%), Gaps = 12/78 (15%)
 Frame = +1

Query: 22     IQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISLMDSNN------------FIEN 69
              I  T Q+N    +L IHK+LP  G++D  I+ +L   S +D+NN                
Sbjct: 703732 IASTKQYN----SLFIHKRLPEYGWSDVQIQSLLFLFSTLDTNNKTLATCGNNEEDDTRW 703899

Query: 70     IGVGEREGRIYSGLVEKR 87
               GVGEREGR++S LV +R
Sbjct: 703900 CGVGEREGRVFSSLVSQR 703953


>scaffold_15
          Length = 1348792

 Score = 30.8 bits (68), Expect = 5.9
 Identities = 17/69 (24%), Positives = 34/69 (49%)
 Frame = -1

Query: 26     SQFNKLLETLLIHKKLPNIGYNDKIIELILNEISLMDSNNFIENIGVGEREGRIYSGLVE 85
              S F   + T++I       G+N   I+ I++EIS+  ++++ +N    + +G +      
Sbjct: 844594 STFQFEVSTMVIMSNTVQAGWNFDNIDSIVDEISVGTNSSYKKNENTRKNDGEMILAKSA 844415

Query: 86     KRHYGFAHG 94
                H G +HG
Sbjct: 844414 TPHTGISHG 844388


>scaffold_21
          Length = 951085

 Score = 30.4 bits (67), Expect = 7.7
 Identities = 18/61 (29%), Positives = 29/61 (47%)
 Frame = -2

Query: 267    HNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYPGRANSSPILDVF 326
              H++ QAC    +  FIQS ++N    + G + SGP       I  N P +   S ++ + 
Sbjct: 900093 HSLLQACSRDILGKFIQSKNQNIKQQLEGLLCSGP--YL*SLILMNEPSKEKESVLISLL 899920

Query: 327    I 327
              I
Sbjct: 899919 I 899917


>scaffold_1
          Length = 5926375

 Score = 30.0 bits (66), Expect = 10.0
 Identities = 23/83 (27%), Positives = 37/83 (44%), Gaps = 1/83 (1%)
 Frame = -2

Query: 295     GAIISGPNSEFIDQISRNYPGRANSSPILDVFITLLSMGKQGWLNLLKERKELLI-YFNE 353
               G I  GPN+   + +   Y  + +     DV+ T+ +  K G  N   +   +LI  +  
Sbjct: 4436544 GNIEEGPNTITYNAVMDAYANKGDVQGASDVYETMKNDFKSGDRNAEVQTYNILIKAWGR 4436365

Query: 354     QLSKFALENNEKLLNTINENKIS 376
                + K A E  EK+L  IN+   S
Sbjct: 4436364 SIKKNAPEEVEKILQEINDQHAS 4436296


  Database: F.cylindrus/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 80,540,407
  Number of sequences in database:  271
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,484,837
Number of Sequences: 271
Number of extensions: 514756
Number of successful extensions: 2105
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1931
Number of HSP's gapped (non-prelim): 428
length of query: 479
length of database: 26,846,802
effective HSP length: 111
effective length of query: 368
effective length of database: 26,816,721
effective search space: 9868553328
effective search space used: 9868553328
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)