TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: C.fasciculata/genome.fa 
           1089 sequences; 37,048,325 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Cf_Contig1068  |  | 1 to 155071                                       268   1e-71
Cf_Contig1057  |  | 1 to 538345                                        31   2.1  
Cf_Contig1047  |  | 1 to 300975                                        29   7.9  

>Cf_Contig1068  |  | 1 to 155071
          Length = 155071

 Score =  268 bits (684), Expect = 1e-71
 Identities = 150/419 (35%), Positives = 235/419 (56%), Gaps = 43/419 (10%)
 Frame = -3

Query: 1     MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
             M+ + L+  + L+   Y+    +      + L +L+  ++ P++G +D +I+L+L +++ 
Sbjct: 18872 MDERGLQLAEDLVSAHYLAAGRESLQATARTLRSLIAQRRCPDVGLSDAVIQLLLRQLAA 18693

Query: 61    MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
             MD+NNF  ++G GEREGR+ S LV +RH+ F HGIGRSGD+  +QPKAAGSSL+ KLT+ 
Sbjct: 18692 MDTNNFAAHVGGGEREGRVASALVRQRHFDFTHGIGRSGDLASEQPKAAGSSLLYKLTNI 18513

Query: 121   LVLDAMKLAGLEQSSLS---NCXXXXXXXXXXXXXXXXXXKSINANNK------------ 165
             +VLD ++LAG   ++ +                       K ++A  +            
Sbjct: 18512 MVLDLIRLAGAPSTAAAVVVPMATGMTLALVLRCVAKTRVKEVDAQQQTAATTTGTTASP 18333

Query: 166   ----RYVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMIRTDLVA-------------- 207
                 RYV+WPRIDQK+ LK I +AGL+P+ +  +    + R+   A              
Sbjct: 18332 RVTPRYVVWPRIDQKTALKCIDSAGLLPLPVELRPAPPLTRSSAEASSTDEGGPAASSSS 18153

Query: 208   ------IEDKIKEL----GVDNILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINNA 257
                   +ED    +    G  +++CV STTSCFAPR+PD  + I+  CK + I +++NNA
Sbjct: 18152 LFLHCDVEDIAATINAVGGPAHVVCVLSTTSCFAPRLPDNTVAIARYCKEHAIPYVVNNA 17973

Query: 258   YGLQCSKILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYPGRA 317
             YG+Q  +I+  I  A +LGRVD  +QS+DKNF+VPVGGA+++  ++E  +  +  Y GRA
Sbjct: 17972 YGVQSRRIMTRIDAAQRLGRVDYVVQSSDKNFLVPVGGAVVTSSSAERCNAAAALYAGRA 17793

Query: 318   NSSPILDVFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFALENNEKLLNTINENKIS 376
             ++SPI+D+FIT LS+G++G   L  ER        +QL  FA    E +L    E  +S
Sbjct: 17792 SASPIIDLFITALSLGRRGMQELWAERYRCRRRLVDQLCGFAKARGEVVLVDDGEGALS 17616



 Score = 49.3 bits (116), Expect = 7e-06
 Identities = 25/71 (35%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
 Frame = -3

Query: 407   IGSKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNY-STSYLTVACAIGITKLEI 465
             +G++LF R+ +G RV+         I G +F+NYG H +   +   L VAC IG+T  E+
Sbjct: 17450 LGAQLFRRAVTGPRVVVPAPRTVTKIAGCDFHNYGMHQEGTPACPLLVVACGIGMTMAEV 17271

Query: 466   DTFIQRLSKLF 476
             +  ++RL +L+
Sbjct: 17270 EGLMERLGELW 17238


>Cf_Contig1057  |  | 1 to 538345
          Length = 538345

 Score = 31.2 bits (69), Expect = 2.1
 Identities = 22/93 (23%), Positives = 44/93 (47%), Gaps = 7/93 (7%)
 Frame = -2

Query: 224    FSTTSCFAPRVPDKIIEISEICKRYNIGHIINNAYGLQCSKILHNISQACKL----GRVD 279
              F  T   A   PD +++      ++++  +  + Y +Q + ILH  SQ C +    G+ +
Sbjct: 127011 FDATYSLAAMEPDAVVQ------QWSLHSLPRDTYSVQNATILHRSSQCCYMIDPQGQAN 126850

Query: 280    AFIQSTDKNFMVPVGGAI---ISGPNSEFIDQI 309
               F++  ++N      G +   ++ PN  F+ QI
Sbjct: 126849 TFVRKLERNRSHGAAGLLQLKLNSPN--FMRQI 126757


>Cf_Contig1047  |  | 1 to 300975
          Length = 300975

 Score = 29.3 bits (64), Expect = 7.9
 Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 7/50 (14%)
 Frame = +2

Query: 70     IGVGEREGRIYSGLVEKRH-------YGFAHGIGRSGDITEQQPKAAGSS 112
              +G    EGR ++   ++R        + F  G  RSG+ TE++ KAAG+S
Sbjct: 200957 LGGAADEGRTHTAAQQRRQRLQCDGVWCFRGGADRSGENTEEREKAAGAS 201106


  Database: C.fasciculata/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 37,048,325
  Number of sequences in database:  1089
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,683,706
Number of Sequences: 1089
Number of extensions: 191262
Number of successful extensions: 714
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 662
Number of HSP's gapped (non-prelim): 116
length of query: 479
length of database: 12,349,441
effective HSP length: 105
effective length of query: 374
effective length of database: 12,235,096
effective search space: 4575925904
effective search space used: 4575925904
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)