TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|81912790|sp|Q80VC6.2|TSAP1_MOUSE RecName: Full=tRNA selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName: Full=tRNA selenocysteine-associated protein 1 (287 letters) Database: genome.fa 2172 sequences; 20,771,095 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAFB02000302.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 83 2e-16 gb|AAFB02000025.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 35 7e-06 gb|AAFB02002138.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 37 0.014 gb|AAFB02000119.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 37 0.014 gb|AAFB02000015.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 37 0.014 gb|AAFB02000549.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 33 0.15 gb|AAFB02000469.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 33 0.15 gb|AAFB02000048.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 33 0.15 gb|AAFB02000199.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 32 0.26 gb|AAFB02000155.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 32 0.26 gb|AAFB02000142.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 32 0.26 gb|AAFB02000072.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 30 0.99 gb|AAFB02000061.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 30 0.99 gb|AAFB02000122.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 30 1.7 gb|AAFB02000094.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 29 2.2 gb|AAFB02000001.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 28 3.8 gb|AAFB02000113.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 28 4.9 >gb|AAFB02000302.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750513136, whole genome shotgun sequence Length = 19880 Score = 82.8 bits (203), Expect = 2e-16 Identities = 53/156 (33%), Positives = 85/156 (54%) Frame = +3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 S+ + + +DE + R F+ +G V+S KI+R++ +G+ AGY FVEF D TA Sbjct: 12537 SVHVSGIHESVDEILLGRIFSIVGH-VVSCKIMRDK-SGVHAGYGFVEFVDSTTARFAKD 12710 Query: 64 KINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSC 123 ++G+ + G + K+N++ Y Q +N Y +FVG L P+V+D +LY+ F K Sbjct: 12711 NMDGRVVYG----RELKVNWS-YTAQQENQGNYKIFVGGLQPEVNDDLLYKTFQKFGRVT 12875 Query: 124 RGGKVVLDPTGVSKGYGFVKFTDELEQKRALTECQG 159 + TG SKGYGFV F + + + A+ G Sbjct: 12876 DARVLKFTQTGKSKGYGFVTFIRKEDAETAMQMMNG 12983 Score = 51.2 bits (121), Expect = 5e-07 Identities = 40/172 (23%), Positives = 78/172 (45%), Gaps = 15/172 (8%) Frame = +3 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 +++G L+P ++++ + + F G V ++++ TG GY FV F AE + Sbjct: 12798 IFVGGLQPEVNDDLLYKTFQKFGR-VTDARVLKFTQTGKSKGYGFVTFIRKEDAETAMQM 12974 Query: 65 INGKPLPGATPAKRFKLNYATYG-----KQPD----------NSPEYSLFVGDLTPDVDD 109 +NG+ L G + K+N+ T +QP +S ++++G++ +V+ Sbjct: 12975 MNGEKLEG----RNIKVNWVTSNIASKTEQPKRSYDEINNETSSQNCTVYIGNIPKNVES 13142 Query: 110 GMLYEFFVKVYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRALTECQGAV 161 L + + Y S ++ D KGY F+KF+ A+ C G + Sbjct: 13143 DDLKQLLAE-YGSIEEVRLNKD-----KGYAFIKFSKHESATSAILMCNGKI 13280 >gb|AAFB02000025.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750508078, whole genome shotgun sequence Length = 74020 Score = 35.0 bits (79), Expect(2) = 7e-06 Identities = 20/46 (43%), Positives = 28/46 (60%), Gaps = 1/46 (2%) Frame = -2 Query: 127 KVVLDP-TGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLS 171 +V+++P T SKGYGFV F+D E AL G +G +P +LS Sbjct: 38658 RVIINPYTNKSKGYGFVSFSDPNEYLLALRTLNGKY-IGTRPCKLS 38524 Score = 31.6 bits (70), Expect(2) = 7e-06 Identities = 17/36 (47%), Positives = 24/36 (66%) Frame = -1 Query: 95 EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVL 130 ++ +FVG+L +VDD ML FF K YPS + K V+ Sbjct: 38803 DFRMFVGNLGKEVDDTMLKIFFSK-YPSVQKVKFVI 38699 >gb|AAFB02002138.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750510476, whole genome shotgun sequence Length = 738 Score = 36.6 bits (83), Expect = 0.014 Identities = 20/53 (37%), Positives = 31/53 (58%) Frame = +3 Query: 15 DENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKING 67 DE+ + R F+ GE + + II++ L GI GY FVE+ + AE+ + NG Sbjct: 246 DESILQRLFSEYGE-IKKIVIIKD-LKGINKGYAFVEYNHRSEAERACRRANG 398 >gb|AAFB02000119.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505371, whole genome shotgun sequence Length = 40781 Score = 36.6 bits (83), Expect = 0.014 Identities = 20/53 (37%), Positives = 31/53 (58%) Frame = +3 Query: 15 DENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKING 67 DE+ + R F+ GE + + II++ L GI GY FVE+ + AE+ + NG Sbjct: 2862 DESILQRLFSEYGE-IKKIVIIKD-LKGINKGYAFVEYNHRSEAERACRRANG 3014 >gb|AAFB02000015.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512038, whole genome shotgun sequence Length = 99283 Score = 36.6 bits (83), Expect = 0.014 Identities = 46/188 (24%), Positives = 68/188 (36%), Gaps = 37/188 (19%) Frame = -3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAE---- 59 S+++G L M EN I F T G +S +++ G G CF+EF D AE Sbjct: 27260 SVFIGKLPKSMKENEIREKFITCGR--ISRMMMQYYPNGQSKGCCFIEFEDKKGAERACS 27087 Query: 60 ---------------------KCL------------HKINGKPLPGATPAKRFKLNYATY 86 KCL H +N K LP T Sbjct: 27086 RDGSIIDKQYIRVNMAEKKPMKCLFSC*TRKYILT*HTVNYKHLPKTTQ----------- 26940 Query: 87 GKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDPTGVSKGYGFVKFTD 146 K+ +NS Y +FVG ++ + L + F K R + T +G+ V+F Sbjct: 26939 -KKMNNS--YCIFVGKVSSITTESTLVKIFAKCGKVLRVTIPIWRNTNKRRGFAVVEFAS 26769 Query: 147 ELEQKRAL 154 ++AL Sbjct: 26768 LASVEKAL 26745 Score = 32.0 bits (71), Expect = 0.34 Identities = 21/69 (30%), Positives = 38/69 (55%), Gaps = 3/69 (4%) Frame = -3 Query: 88 KQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSC-RGGKVVLD--PTGVSKGYGFVKF 144 +Q +PE S+F+G L + + + E F+ +C R ++++ P G SKG F++F Sbjct: 27287 RQKPYNPETSVFIGKLPKSMKENEIREKFI----TCGRISRMMMQYYPNGQSKGCCFIEF 27120 Query: 145 TDELEQKRA 153 D+ +RA Sbjct: 27119 EDKKGAERA 27093 >gb|AAFB02000549.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505155, whole genome shotgun sequence Length = 9256 Score = 33.1 bits (74), Expect = 0.15 Identities = 27/71 (38%), Positives = 35/71 (49%), Gaps = 6/71 (8%) Frame = +3 Query: 92 NSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDPT------GVSKGYGFVKFT 145 N LFVG+L + + L EFF R GKVV D G SKG+G+V+F Sbjct: 5679 NKMNRRLFVGNLEYQIKNFELKEFFE------RFGKVV-DVVIMERYDGASKGFGYVEFD 5837 Query: 146 DELEQKRALTE 156 E ++AL E Sbjct: 5838 TEESAQKALRE 5870 Score = 29.6 bits (65), Expect = 1.7 Identities = 25/88 (28%), Positives = 39/88 (44%), Gaps = 1/88 (1%) Frame = +3 Query: 95 EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-PTGVSKGYGFVKFTDELEQKRA 153 E +FV +L +D L + F + VV D T SKGYGFV F ++Q A Sbjct: 6378 ETVIFVRNLPFSMDSFELKQLFEPCHSI--DASVVYDRDTNKSKGYGFVVFDTSIDQHTA 6551 Query: 154 LTECQGAVGLGCKPVRLSVAIPKASRVK 181 + + + G K + ++ + R K Sbjct: 6552 IEQFNNYLVEGRKIMVFKASLLQEERTK 6635 >gb|AAFB02000469.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750508508, whole genome shotgun sequence Length = 11701 Score = 33.1 bits (74), Expect = 0.15 Identities = 18/51 (35%), Positives = 29/51 (56%) Frame = -2 Query: 128 VVLDPTGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLSVAIPKAS 178 ++++ G+SKG GFV F +E K+A+ E G G + L V++ K S Sbjct: 1035 ILMEKKGISKGCGFVDFENEENAKKAVEEMNGR---GVEGGELEVSMAKIS 892 >gb|AAFB02000048.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750504825, whole genome shotgun sequence Length = 63580 Score = 33.1 bits (74), Expect = 0.15 Identities = 19/57 (33%), Positives = 32/57 (56%) Frame = +2 Query: 98 LFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRAL 154 ++V L VD+ +LYE F+ + ++ D ++KGYGFV F DE + +A+ Sbjct: 18707 IYVSGLGDFVDEQLLYEVFLP-FGEITDIQLPKDNGKLNKGYGFVVFEDEEDASQAI 18874 >gb|AAFB02000199.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505961, whole genome shotgun sequence Length = 29416 Score = 32.3 bits (72), Expect = 0.26 Identities = 23/87 (26%), Positives = 38/87 (43%) Frame = -1 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++ + P +E + F G+ + SV+IIR+R + GY F+EF E K Sbjct: 19126 LFICKMNPITNEEDLEEIFRKYGK-IRSVEIIRDRKSKKSLGYGFIEFETKEGCENAYQK 18950 Query: 65 INGKPLPGATPAKRFKLNYATYGKQPD 91 ++ + F + YG Q D Sbjct: 18949 MDNVIIDERRIHVDFSQSIGKYGVQYD 18869 >gb|AAFB02000155.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750516041, whole genome shotgun sequence Length = 34370 Score = 32.3 bits (72), Expect = 0.26 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = -3 Query: 127 KVVLDPTGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLSVAIPKASR 179 +V G SKG+GFV F DE ++A+ E G K + S+ + + SR Sbjct: 12330 RVATHKDGASKGFGFVTFNDEETAEKAVNEMNG------KEIDGSIVVVQISR 12190 Score = 32.0 bits (71), Expect = 0.34 Identities = 14/32 (43%), Positives = 21/32 (65%) Frame = -3 Query: 42 GIPAGYCFVEFADLATAEKCLHKINGKPLPGA 73 G G+ FV F D TAEK ++++NGK + G+ Sbjct: 12309 GASKGFGFVTFNDEETAEKAVNEMNGKEIDGS 12214 >gb|AAFB02000142.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750513556, whole genome shotgun sequence Length = 36110 Score = 32.3 bits (72), Expect = 0.26 Identities = 23/68 (33%), Positives = 33/68 (48%), Gaps = 6/68 (8%) Frame = +3 Query: 95 EYSLFVGDLTPDVDDGMLYEFFVKVYP------SCRGGKVVLDPTGVSKGYGFVKFTDEL 148 E LF+ +L ++D L E F K P S R G + SKG+GFVK ++ Sbjct: 6585 ETLLFIRNLPYSINDEKLKELFAKYEPKEAVVVSYRKGSRIF-----SKGFGFVKVDNKE 6749 Query: 149 EQKRALTE 156 +Q A+ E Sbjct: 6750 KQSAAIVE 6773 >gb|AAFB02000072.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750513744, whole genome shotgun sequence Length = 50435 Score = 30.4 bits (67), Expect = 0.99 Identities = 25/84 (29%), Positives = 41/84 (48%) Frame = +2 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 S+ + +L+ + I + F+ GE +K IR T + F+EF DL +AEK L Sbjct: 24614 SIVVFNLDETITNTLIHQIFSQFGE----IKDIRQ--TPNKKHHRFIEFFDLRSAEKALK 24775 Query: 64 KINGKPLPGATPAKRFKLNYATYG 87 +N L G K+ K+ ++ G Sbjct: 24776 TMNKSELNG----KKLKIEFSRPG 24835 >gb|AAFB02000061.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750513836, whole genome shotgun sequence Length = 54062 Score = 30.4 bits (67), Expect = 0.99 Identities = 22/74 (29%), Positives = 35/74 (47%) Frame = +3 Query: 30 VMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQ 89 V+ V I N+ T GY +EF + EK + K+ G L G K+ T+ K+ Sbjct: 33912 VLKVNIPINQFTQTTMGYANIEFKNSNAVEKAI-KLTGTNLNGRYIEVSKKI---TFDKR 34079 Query: 90 PDNSPEYSLFVGDL 103 ++ ++S F DL Sbjct: 34080 KNDRTDFSWFFNDL 34121 >gb|AAFB02000122.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750511394, whole genome shotgun sequence Length = 40251 Score = 29.6 bits (65), Expect = 1.7 Identities = 21/52 (40%), Positives = 28/52 (53%), Gaps = 2/52 (3%) Frame = -3 Query: 107 VDDGMLYEFFVKVYPSCRG-GKVVLDPTGV-SKGYGFVKFTDELEQKRALTE 156 V D +L+ FF K GKVV D SKG+GFV F + + K+A+ E Sbjct: 17521 VTDCLLFNFF*KKRKE*LDIGKVVTDRDSQRSKGFGFVTFEKDEDAKKAIEE 17366 >gb|AAFB02000094.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514637, whole genome shotgun sequence Length = 45027 Score = 29.3 bits (64), Expect = 2.2 Identities = 29/108 (26%), Positives = 47/108 (43%), Gaps = 7/108 (6%) Frame = -3 Query: 56 ATAEKCLHKINGKPLPGATPAKRFKLNYATYG-KQPDNSPEYSLFVGDLTPDVDDGMLYE 114 A AE ++ G+ + A K+ + T +P + E ++FV +L + D + Sbjct: 14728 AIAEMNNFEVEGRKITVAAAYKKVENKQTTKKTNEPKSLSETNVFVKNLPFTLTDEGFKK 14549 Query: 115 FFVK------VYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRALTE 156 F K K+ +D SKGYGFV F +QK+A+ E Sbjct: 14548 LFEKYDVVEATIVKRHNKKLNVDR---SKGYGFVTFKTAEQQKKAIAE 14414 Score = 27.3 bits (59), Expect = 8.4 Identities = 43/182 (23%), Positives = 67/182 (36%), Gaps = 29/182 (15%) Frame = -3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL- 62 +L++ L+ + + AF T VKII + G G+ F+EFA AEK L Sbjct: 15238 TLYVSHLDYSLKVEDVQNAFKTYNPK--EVKIISTPI-GYSRGFAFIEFATPQEAEKALE 15068 Query: 63 ---HKIN------GKPLPGATPAKRFKLNYATYG-----------KQPDNSPEYSLFVGD 102 H I K LP K+N + K+ + + LF+ + Sbjct: 15067 MDRHTIGKMEIKVQKALPKEETTTERKMNRRGFKGKRNTRKRHLVKRTNEVTDNMLFIKN 14888 Query: 103 LTPDVDDGMLYEFFVK--------VYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRAL 154 L + + L E F K + + G V + G F+ EQK+A+ Sbjct: 14887 LPFAITEEKLKEMFSKFGVVEITLIKTHRKKGNVT-----KNNGIAFITVKTAEEQKKAI 14723 Query: 155 TE 156 E Sbjct: 14722 AE 14717 >gb|AAFB02000001.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750515629, whole genome shotgun sequence Length = 192445 Score = 28.5 bits (62), Expect = 3.8 Identities = 21/71 (29%), Positives = 31/71 (43%) Frame = -1 Query: 48 CFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDV 107 C ++ +AT EKC+ + K + F L Y P+ E + D P + Sbjct: 90940 CDLKIIQMATLEKCIQTMMSKDFNDPYFQECFVLAYRLV-TTPEKLLELLSILFD--PSI 90770 Query: 108 DDGMLYEFFVK 118 DGM +E FVK Sbjct: 90769 PDGMEWEVFVK 90737 >gb|AAFB02000113.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750506007, whole genome shotgun sequence Length = 41641 Score = 28.1 bits (61), Expect = 4.9 Identities = 26/109 (23%), Positives = 40/109 (36%), Gaps = 8/109 (7%) Frame = -1 Query: 9 DLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGK 68 +L +DE + R F+ GE + I+ G+ G C V+FAD A +N Sbjct: 25942 NLPKILDERDLRRYFSQYGE--LKGLAIKKTDYGVSKGTCVVKFADCVVARIVAETLNNH 25769 Query: 69 PLPG--------ATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDD 109 L +RF Y +Q P+ + DVD+ Sbjct: 25768 ILENRLLRTELCEAKKRRFHSTYPVSKEQRRKEPQIKVIT---QKDVDE 25631 Database: genome.fa Posted date: Jan 19, 2010 5:04 PM Number of letters in database: 20,771,095 Number of sequences in database: 2172 Lambda K H 0.317 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,083,918 Number of Sequences: 2172 Number of extensions: 43363 Number of successful extensions: 112 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 52 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 42 Number of HSP's gapped (non-prelim): 74 length of query: 287 length of database: 6,923,698 effective HSP length: 96 effective length of query: 191 effective length of database: 6,715,186 effective search space: 1282600526 effective search space used: 1282600526 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)