TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|8923460|ref|NP_060316.1| tRNA selenocysteine 1-associated protein 1 [Homo sapiens] (287 letters) Database: genome.fa 9 sequences; 8,347,606 total letters Searching.........done Score E Sequences producing significant alignments: (bits) Value gb|AAGK01000001.1| Theileria parva strain Muguga chromosome 1 ch... 71 3e-13 gb|AAGK01000002.1| Theileria parva strain Muguga chromosome 2 ch... 49 2e-06 gb|AAGK01000004.1| Theileria parva strain Muguga chromosome 4 ct... 46 1e-05 >gb|AAGK01000001.1| Theileria parva strain Muguga chromosome 1 chr1_complete, whole genome shotgun sequence Length = 2540030 Score = 70.9 bits (172), Expect = 3e-13 Identities = 51/180 (28%), Positives = 90/180 (50%), Gaps = 2/180 (1%) Frame = -3 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 +ASL++GDL+P + E + F T+G V S+++ R+ +T GY +V + A++ Sbjct: 1962567 SASLYVGDLKPDVTEAVLYEVFNTVG-PVASIRVCRDSVTRKSLGYAYVNYYSTQDAQEA 1962391 Query: 62 LHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYP 121 L +N + G ++ ++ S ++FV +L +D LY+ F P Sbjct: 1962390 LENLNYIEIKGHPT----RIMWSNRDPSLRRSGAGNIFVKNLDKSIDTKSLYDTFSHFGP 1962223 Query: 122 --SCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179 SC KV +D +G SK YGFV + +E + A+ + G + +G K V ++ + K R Sbjct: 1962222 ILSC---KVAVDASGASKRYGFVHYENEESAREAIEKVNGML-IGGKRVEVAPFLRKQDR 1962055 Score = 38.1 bits (87), Expect = 0.002 Identities = 30/135 (22%), Positives = 59/135 (43%), Gaps = 3/135 (2%) Frame = +1 Query: 23 FATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL---HKINGKPLPGATPAKRF 79 F+ GE V +I+R++ +G G+ FV FA+ + L H I+G ++ Sbjct: 1837939 FSKFGE-VTHTEIVRDKNSGRSRGFGFVTFAERESVNTVLRKSHTIDGVEADVKLAVRKE 1838115 Query: 80 KLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGY 139 K P +FVG ++ + + ++F + P +V T +GY Sbjct: 1838116 KAKILA----PQYDQTKRIFVGGVSEKITESYFRDYFARYGPIASYNYLVDRATNRPRGY 1838283 Query: 140 GFVKFTDELEQKRAL 154 GFV + + + ++++ Sbjct: 1838284 GFVIYENMEDAEKSV 1838328 Score = 37.7 bits (86), Expect = 0.003 Identities = 27/98 (27%), Positives = 48/98 (48%), Gaps = 2/98 (2%) Frame = -3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 SL++ + P + N + R F+ G V + +I +R T GY FV + + +A + + Sbjct: 1030629 SLFIFHIPPQWNNNDLFRTFSPFGRVVQA-RIAIDRSTNRSKGYAFVSYDNPESATQAVA 1030453 Query: 64 KINGKPLPGATPAKRFKLNYATYGKQ--PDNSPEYSLF 99 +NG + G K+ ++NY T + P +YS F Sbjct: 1030452 NMNGFTIMG----KKLRVNYKTTNNRSNPY*ISDYSFF 1030351 Score = 33.5 bits (75), Expect = 0.050 Identities = 29/87 (33%), Positives = 38/87 (43%), Gaps = 13/87 (14%) Frame = -1 Query: 98 LFVGDLTPDVDDGMLYEFFVKVY-PSCRGGKVVLDQT------------GVSKGYGFVKF 144 LFV +L ++ L FF +Y P ++VLD+T G KGYG+VKF Sbjct: 914087 LFVTNLDVEITPDALDNFFQYIYGPLTSKTRLVLDETTGTKLLFSEFGIGRHKGYGYVKF 913908 Query: 145 TDELEQKRALTECQGAVGLGSKPVRLS 171 + R L G LG K V S Sbjct: 913907 STPDIATRTLLTLNG-TRLGDKHVHFS 913830 Score = 29.6 bits (65), Expect = 0.72 Identities = 23/94 (24%), Positives = 40/94 (42%), Gaps = 9/94 (9%) Frame = -3 Query: 82 NYATYGKQPDNS--------PEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ- 132 NY YG N+ + SLF+ + P ++ L+ F + ++ +D+ Sbjct: 1030698 NYNNYGHSGGNNYNNNSGSGGDNSLFIFHIPPQWNNNDLFRTF-SPFGRVVQARIAIDRS 1030522 Query: 133 TGVSKGYGFVKFTDELEQKRALTECQGAVGLGSK 166 T SKGY FV + + +A+ G +G K Sbjct: 1030521 TNRSKGYAFVSYDNPESATQAVANMNGFTIMGKK 1030420 >gb|AAGK01000002.1| Theileria parva strain Muguga chromosome 2 chr2_complete, whole genome shotgun sequence Length = 1971884 Score = 48.5 bits (114), Expect = 2e-06 Identities = 30/77 (38%), Positives = 46/77 (59%), Gaps = 1/77 (1%) Frame = +1 Query: 95 EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRA 153 +Y +F GDL +V D +L F K YPS + +V+ D+ +G S+GYGFV + E A Sbjct: 535294 DYRIFCGDLGNEVTDEILANAF-KRYPSFQRARVIRDKNSGKSRGYGFVSLLNPNEMLTA 535470 Query: 154 LTECQGAVGLGSKPVRL 170 L E A +G++P+R+ Sbjct: 535471 LKEMNHAF-VGNRPIRV 535518 Score = 33.5 bits (75), Expect = 0.050 Identities = 36/143 (25%), Positives = 61/143 (42%), Gaps = 38/143 (26%) Frame = +2 Query: 46 GYCFVEFADLATAEKCLHKINGK-------PLPGATPAKRF------------KLNYATY 86 GY FV F+ L ++K ++++NGK L + P + + +N A Sbjct: 1897967 GYAFVNFSSLEKSKKLINELNGKLFKNRRIQLSLSLPKELYLSKSTSEATDNDTINTAET 1898146 Query: 87 GKQP--DNSPEYS---------------LFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVV 129 P D +PE + +FV +L + + L+E+F K + + Sbjct: 1898147 NGTPQGDTTPEDNVGNRDEHQKGDIKDTIFVRNLDYECTEKELFEYFSK-FAEVDSCNIC 1898323 Query: 130 LDQTGVSKGYGFVKF--TDELEQ 150 L++ SKG FVKF D+L+Q Sbjct: 1898324 LNEDKTSKGTAFVKFKNKDQLKQ 1898392 Score = 30.8 bits (68), Expect = 0.33 Identities = 20/61 (32%), Positives = 34/61 (55%) Frame = +2 Query: 116 FVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIP 175 F K Y +G KV + SKGY FV F+ + K+ + E G + ++ ++LS+++P Sbjct: 1897913 FFKEYD--KGAKVHIPSH--SKGYAFVNFSSLEKSKKLINELNGKL-FKNRRIQLSLSLP 1898077 Query: 176 K 176 K Sbjct: 1898078 K 1898080 Score = 30.0 bits (66), Expect = 0.55 Identities = 21/72 (29%), Positives = 41/72 (56%), Gaps = 3/72 (4%) Frame = +1 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEF---ADLATAEK 60 +L++ ++ + E +S+ F T G+ + V++I +R + P GY F+E+ D+ TA K Sbjct: 1099789 TLFVANIAYDVTEKQLSKEFQTYGK-IRRVRMIHDR-SNKPRGYAFIEYENERDMVTAYK 1099962 Query: 61 CLHKINGKPLPG 72 + +GK + G Sbjct: 1099963 ---RADGKKISG 1099989 >gb|AAGK01000004.1| Theileria parva strain Muguga chromosome 4 ctg_529, whole genome shotgun sequence Length = 1835834 Score = 45.8 bits (107), Expect = 1e-05 Identities = 36/117 (30%), Positives = 56/117 (47%), Gaps = 1/117 (0%) Frame = +1 Query: 46 GYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTP 105 GYCF+EF A+ K++G G + R L++ NS + +FV ++ Sbjct: 63247 GYCFLEFKTPELADLAF-KLDGITCNGYSLKLRRPLDF----NLGTNSDDTKVFVQNIPL 63411 Query: 106 DVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRALTECQGAV 161 DV + + E K + + ++ D TGVSKGYGF +F D K A+ G+V Sbjct: 63412 DVTEDQMKELLEK-HGKLKLANLLKDPATGVSKGYGFFEFEDARSSKLAVLHLNGSV 63579 Score = 32.3 bits (72), Expect = 0.11 Identities = 17/64 (26%), Positives = 29/64 (45%) Frame = -1 Query: 84 ATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVK 143 A+ + S ++ +L D+ L EF + + K+ D G SKGY FV+ Sbjct: 1780628 ASSHSEVSGSVHREFYLCNLNYSTDETRLREFLESKFGPVKSLKICRDSAGKSKGYAFVE 1780449 Query: 144 FTDE 147 F ++ Sbjct: 1780448 FVND 1780437 Score = 28.1 bits (61), Expect = 2.1 Identities = 15/57 (26%), Positives = 26/57 (45%), Gaps = 1/57 (1%) Frame = +1 Query: 35 IIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPL-PGATPAKRFKLNYATYGKQP 90 ++++ TG+ GY F EF D +++ + +NG L K Y G +P Sbjct: 63475 LLKDPATGVSKGYGFFEFEDARSSKLAVLHLNGSVLGKNVLSVKHAAFGYFASGGKP 63645 Score = 26.6 bits (57), Expect = 6.1 Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 5/58 (8%) Frame = -1 Query: 23 FATMGETVMSVKIIRNRLTGIPAGYCFVEF-----ADLATAEKCLHKINGKPLPGATP 75 F++ G+ ++++ + T G+CFVE+ ADLA I GKP+ A P Sbjct: 1074266 FSSFGD-ILNIDLPTEPDTNKVKGFCFVEYRKKESADLALNSMQGFHIKGKPIKLARP 1074096 Database: genome.fa Posted date: Jan 19, 2010 5:27 PM Number of letters in database: 8,347,606 Number of sequences in database: 9 Lambda K H 0.317 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,871,264 Number of Sequences: 9 Number of extensions: 21229 Number of successful extensions: 98 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 58 Number of HSP's gapped (non-prelim): 67 length of query: 287 length of database: 2,782,535 effective HSP length: 90 effective length of query: 197 effective length of database: 2,781,725 effective search space: 547999825 effective search space used: 547999825 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)