TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|224465209|ref|NP_699167.2| L-seryl-tRNA(Sec) kinase [Homo
sapiens]
         (348 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_43                                                            32   2.0  
scaffold_7                                                             31   3.4  

>scaffold_43
          Length = 481750

 Score = 31.6 bits (70), Expect = 2.0
 Identities = 12/33 (36%), Positives = 21/33 (63%)
 Frame = -2

Query: 133   SCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQ 165
             SCY++TKT +++  +L    N Y   +RY++ Q
Sbjct: 98964 SCYIITKTFINKKTYLAK*INLYEYRLRYKIIQ 98866


>scaffold_7
          Length = 741107

 Score = 30.8 bits (68), Expect = 3.4
 Identities = 31/158 (19%), Positives = 55/158 (34%)
 Frame = +2

Query: 76     WKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQSCY 135
              W +LR  L++   +F +           P++  A+  D I  ++    I+          
Sbjct: 620465 WLILRGNLIRIFRFFCI-----------PSKFLALMNDIIQFIQITTAIYILEQRQTIQQ 620611

Query: 136    LLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLQRNGQRPQ 195
               L  T   + + L   +   Y  ++  +   ++  S  F  L   C  E C  +N   P 
Sbjct: 620612 QLQSTKEKKLISLKCPNLISYSELQETIQSSSKVTSQKFEFLMNICKTELCQDQNSSHPS 620791

Query: 196    ALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPACAS 233
                  + +H+M  K      +K  W  N     S  C S
Sbjct: 620792 NSKRQILHIMNCK------DKLLWMFNRQICKS*YCQS 620887


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.321    0.134    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,350,858
Number of Sequences: 697
Number of extensions: 346690
Number of successful extensions: 1232
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1170
Number of HSP's gapped (non-prelim): 147
length of query: 348
length of database: 23,960,378
effective HSP length: 107
effective length of query: 241
effective length of database: 23,885,799
effective search space: 5756477559
effective search space used: 5756477559
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 64 (29.3 bits)