TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|224465209|ref|NP_699167.2| L-seryl-tRNA(Sec) kinase [Homo
sapiens]
         (348 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000097.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    33   0.20 
gb|AAFB02000053.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    31   0.76 
gb|AAFB02000162.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   6.5  
gb|AAFB02000063.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   6.5  
gb|AAFB02000037.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   6.5  
gb|AAFB02000408.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   8.4  

>gb|AAFB02000097.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750510384, whole genome
             shotgun sequence
          Length = 44906

 Score = 33.1 bits (74), Expect = 0.20
 Identities = 19/79 (24%), Positives = 37/79 (46%)
 Frame = -2

Query: 142   VSRPLFLVLDDNFYYQSMRYEVYQLARKYSLGFCQLFLDCPLETCLQRNGQRPQALPPET 201
             ++R   ++LD   Y +S RYE+    +  +     + +  P E C++ N QR +    E 
Sbjct: 30598 LNRETIVILDSLNYNKSFRYELKCHCKSIASTSVTISVTAPKEKCIEWNAQREEKYSDEM 30419

Query: 202   IHLMGRKLEKPNPEKNAWE 220
             +  +  + E P P  + W+
Sbjct: 30418 VVELIERYEVPLP-SHKWD 30365


>gb|AAFB02000053.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750508094, whole genome
             shotgun sequence
          Length = 56913

 Score = 31.2 bits (69), Expect = 0.76
 Identities = 17/57 (29%), Positives = 26/57 (45%)
 Frame = +1

Query: 109   AMWEDFITCLKDQDLIFSAAFEAQSCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQ 165
             A++  FI  L   D +++  F  Q    L    +   L   LD    +Q M+YE+YQ
Sbjct: 49672 ALYYHFIILLYFLDYLYTVFFSFQESRALLNNTIKYYLIYSLD*KRVFQHMKYEIYQ 49842


>gb|AAFB02000162.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750507776, whole genome
             shotgun sequence
          Length = 33219

 Score = 28.1 bits (61), Expect = 6.5
 Identities = 19/79 (24%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
 Frame = -1

Query: 205   MGRKLEKPNPEKNAWEHNSLTIPSPACASEASLEVTDLL--LTALENPVKYAEDNMEQKD 262
             +GR  +K  P+KNA E+  +T P      +  +++  ++  +      +   E +++Q D
Sbjct: 21732 LGRSRDKAKPQKNASEYLIVTEPENELIEKEDVQLKKVIENMHPDNEDLGIKESDLQQSD 21553

Query: 263   TDRIICSTNILHKTDQTLR 281
                I  +T I     Q LR
Sbjct: 21552 PVWIKFNTKIAKDPSQVLR 21496


>gb|AAFB02000063.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505997, whole genome
             shotgun sequence
          Length = 53009

 Score = 28.1 bits (61), Expect = 6.5
 Identities = 27/107 (25%), Positives = 48/107 (44%), Gaps = 4/107 (3%)
 Frame = -2

Query: 67    ARARPAPSQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITC----LKDQD 122
             A+ R +P +   LR+E  KY++  +  V+N C   V     E      + C    L    
Sbjct: 36529 AKKRISPFRVLPLRKEAEKYIQAIMGVVVN-CPAKVEKEEIERRARKVVFCAAHSLPITY 36353

Query: 123   LIFSAAFEAQSCYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARK 169
              I  + FE +  Y L+ + +   L L+++D F + ++  E    A+K
Sbjct: 36352 RILDSFFEDKEDY-LSLSVMYNALHLIVNDRFLWINVEEEKEIHAKK 36215


>gb|AAFB02000037.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750506525, whole genome
            shotgun sequence
          Length = 70380

 Score = 28.1 bits (61), Expect = 6.5
 Identities = 15/37 (40%), Positives = 21/37 (56%)
 Frame = -2

Query: 4    AENIRGTGSDGPRKRGLCVLCGLPAAGKSTFARALAH 40
            +E  +  G D PR    C+L G P  GK+  A+A+AH
Sbjct: 9662 SELYKQIGIDPPRG---CLLYGPPGTGKTMLAKAVAH 9561


>gb|AAFB02000408.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750507250, whole genome
            shotgun sequence
          Length = 14404

 Score = 27.7 bits (60), Expect = 8.4
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +2

Query: 22   VLCGLPAAGKSTFARALAHRLQQEQ 46
            +LCG P  GK+T    LAH L +++
Sbjct: 7337 LLCGQPGIGKTTSIHCLAHELLKDR 7411


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.321    0.134    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,251,283
Number of Sequences: 2172
Number of extensions: 100948
Number of successful extensions: 400
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 283
Number of HSP's gapped (non-prelim): 164
length of query: 348
length of database: 6,923,698
effective HSP length: 98
effective length of query: 250
effective length of database: 6,710,842
effective search space: 1677710500
effective search space used: 1677710500
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)