TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|224465209|ref|NP_699167.2| L-seryl-tRNA(Sec) kinase [Homo
sapiens]
         (348 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_278                                                           52   5e-07
scaffold_54                                                            30   2.7  

>scaffold_278 
          Length = 44317

 Score = 52.4 bits (124), Expect = 5e-07
 Identities = 26/83 (31%), Positives = 45/83 (54%), Gaps = 7/83 (8%)
 Frame = -3

Query: 148   LVLDDNFYYQSMRYEVYQLARKY-SLGFCQLFLDCPLETCLQRNGQRP------QALPPE 200
             ++LDDN YY+SMRY+ YQL+  + ++ +CQ++    +   ++ N  R         +  E
Sbjct: 25130 IILDDNMYYKSMRYKYYQLSNSFQNVKYCQIYFKKDIYKSIENNNNRTINNQNHNNVNNE 24951

Query: 201   TIHLMGRKLEKPNPEKNAWEHNS 223
              I  M   +E PN +K  WE ++
Sbjct: 24950 IIKKMNEIIEPPNVDKYRWEQDN 24882


>scaffold_54 
          Length = 110490

 Score = 30.0 bits (66), Expect = 2.7
 Identities = 17/70 (24%), Positives = 35/70 (50%)
 Frame = -2

Query: 217    NAWEHNSLTIPSPACASEASLEVTDLLLTALENPVKYAEDNMEQKDTDRIICSTNILHKT 276
              NAW    L +PSP+ A+E     ++ L+   E  +   ++  E+K+         ++ K 
Sbjct: 101813 NAWSTGKLLVPSPSIATEKKSNSSEKLIE--EKQIVEKKEKKEKKEKP-------VVEKK 101661

Query: 277    DQTLRRIVSQ 286
              +Q ++R+ S+
Sbjct: 101660 EQPMKRVNSK 101631


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.321    0.134    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,795,872
Number of Sequences: 799
Number of extensions: 166485
Number of successful extensions: 604
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 550
Number of HSP's gapped (non-prelim): 102
length of query: 348
length of database: 10,989,169
effective HSP length: 101
effective length of query: 247
effective length of database: 10,908,470
effective search space: 2694392090
effective search space used: 2694392090
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)