TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName:
Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec)
kinase; Short=PSTK
         (255 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_58                                                            33   0.43 
scaffold_88                                                            32   1.3  
scaffold_31                                                            31   2.2  
scaffold_86                                                            30   4.8  
scaffold_67                                                            29   6.3  
scaffold_76                                                            29   6.3  
scaffold_40                                                            29   8.2  
scaffold_120                                                           29   8.2  

>scaffold_58
          Length = 446585

 Score = 33.1 bits (74), Expect = 0.43
 Identities = 18/61 (29%), Positives = 33/61 (54%), Gaps = 4/61 (6%)
 Frame = -2

Query: 47    KESYEEFIRDSNNYL----IKEALENKFSVIVDDTNYYNSKRRDLMNIAKECDTNYVTIY 102
             K++    +RD+N  L     K+ ++NKF  I    N+      D+++I   CD+ Y++IY
Sbjct: 29230 KKTINITLRDNNLLLKSI*FKKFIQNKFCSIKLQKNFLKFLSFDMIHIE*SCDSTYISIY 29051

Query: 103   L 103
             +
Sbjct: 29050 I 29048


>scaffold_88
          Length = 342040

 Score = 31.6 bits (70), Expect = 1.3
 Identities = 12/34 (35%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
 Frame = -1

Query: 85     RDLMNIAKECDTNYVTIYL-KAPLNLLLKRNIER 117
              RDL  + ++ DTN+ T+YL ++P   ++ RN ++
Sbjct: 221545 RDLTELGRDTDTNFATLYLRRSPFTEIINRNYQK 221444


>scaffold_31
          Length = 528592

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 27/111 (24%), Positives = 48/111 (43%)
 Frame = +1

Query: 23     KMAEKNIDNIILGTDLIRESFPVWKESYEEFIRDSNNYLIKEALENKFSVIVDDTNYYNS 82
              K+   N+  +   T LI  S  +W ES  + +       ++E L+  F+ I DD++Y   
Sbjct: 335350 KLVFSNLK*LSHSTSLIL*SIRIWLESKNQIVIQQIRLYLEEYLKTSFN*IHDDSDYIPM 335529

Query: 83     KRRDLMNIAKECDTNYVTIYLKAPLNLLLKRNIERGQKIPNEVIKNMYEKF 133
                + L N      +N      KA       +++ + Q   N+ +KN Y+ F
Sbjct: 335530 LFQILCNY-----SNLFKPQFKA-------KDL*KNQIFLNQSLKN*YKSF 335646


>scaffold_86
          Length = 349122

 Score = 29.6 bits (65), Expect = 4.8
 Identities = 21/63 (33%), Positives = 31/63 (49%), Gaps = 5/63 (7%)
 Frame = +3

Query: 50     YEEFIRDSNNYLIKEALENKFSVIVDDTNYY-----NSKRRDLMNIAKECDTNYVTIYLK 104
              Y + ++ SNNY+I  A E+   +     NYY     N K+  +MNI K    +Y   YL+
Sbjct: 274962 YSKILKLSNNYIIPYAAESD-DINGATYNYYIFGNRNRKQFAIMNILKGTFDSYAFNYLR 275138

Query: 105    APL 107
                L
Sbjct: 275139 TDL 275147


>scaffold_67
          Length = 405785

 Score = 29.3 bits (64), Expect = 6.3
 Identities = 15/39 (38%), Positives = 26/39 (66%)
 Frame = +1

Query: 1      MLIILTGLPSVGKSTFSKAFSKKMAEKNIDNIILGTDLI 39
              M+II+ G PS+GKS  +   ++++   NI N +L TD++
Sbjct: 323035 MIIIIFGAPSIGKSLLANNLAERL---NISN-VLQTDIV 323139


>scaffold_76
          Length = 362286

 Score = 29.3 bits (64), Expect = 6.3
 Identities = 15/39 (38%), Positives = 26/39 (66%)
 Frame = +1

Query: 1      MLIILTGLPSVGKSTFSKAFSKKMAEKNIDNIILGTDLI 39
              M+II+ G PS+GKS  +   ++++   NI N +L TD++
Sbjct: 350461 MIIIIFGAPSIGKSLLANNLAERL---NISN-VLQTDIV 350565


>scaffold_40
          Length = 494621

 Score = 28.9 bits (63), Expect = 8.2
 Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -2

Query: 85     RDLMNIAKECDTNYVTIYL-KAPLNLLLKRNIER 117
              RDL  + +  D+ Y TIYL ++P   ++ RN E+
Sbjct: 195280 RDLTELGRNTDSIYATIYLRRSPFTEIIDRNYEK 195179


>scaffold_120
          Length = 280595

 Score = 28.9 bits (63), Expect = 8.2
 Identities = 11/24 (45%), Positives = 16/24 (66%)
 Frame = +3

Query: 81    NSKRRDLMNIAKECDTNYVTIYLK 104
             NS  RDL  + ++ D+NY  IYL+
Sbjct: 89670 NSDYRDLTELGRDTDSNYAVIYLR 89741


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.315    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,876,240
Number of Sequences: 697
Number of extensions: 230583
Number of successful extensions: 869
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 790
Number of HSP's gapped (non-prelim): 209
length of query: 255
length of database: 23,960,378
effective HSP length: 103
effective length of query: 152
effective length of database: 23,888,587
effective search space: 3631065224
effective search space used: 3631065224
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 62 (28.5 bits)