TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: genome.fa 5578 sequences; 78,051,097 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQY01000949.1| Phytophthora sojae strain P6497 Psojae_Cont94... 35 0.093 gb|AAQY01000221.1| Phytophthora sojae strain P6497 Psojae_Cont22... 29 6.6 >gb|AAQY01000949.1| Phytophthora sojae strain P6497 Psojae_Cont949, whole genome shotgun sequence Length = 166052 Score = 35.4 bits (80), Expect = 0.093 Identities = 23/92 (25%), Positives = 41/92 (44%) Frame = -3 Query: 2 LIILTGLPSVGKSTFSKAFSKKMAEKNIDNIILGTDLIRESFPVWKESYEEFIRDSNNYL 61 +++L G P+ GKS F+K + ++D + + ++ Sbjct: 48060 MVVLVGPPASGKSFFAKTYLSSYTVVSLDELRTAANCKKKCL------------------ 47935 Query: 62 IKEALENKFSVIVDDTNYYNSKRRDLMNIAKE 93 EA+ K SV+VD+TN R++ + IAKE Sbjct: 47934 --EAIAEKKSVVVDNTNREPRARKEWITIAKE 47845 >gb|AAQY01000221.1| Phytophthora sojae strain P6497 Psojae_Cont221, whole genome shotgun sequence Length = 399879 Score = 29.3 bits (64), Expect = 6.6 Identities = 25/115 (21%), Positives = 54/115 (46%), Gaps = 4/115 (3%) Frame = +3 Query: 6 TGLPSVGKSTFSKAFSKKMAEKNIDNIILGTDLIRESFPVWKESYEEFIRDSNNYLIKE- 64 TGL GKST +A K++ + + L D +R + E R + + E Sbjct: 258900 TGLSGSGKSTVGRALEKELLHRRVHTYRLDGDNVRIGLN-RDLGFSETDRAESVRRVGEM 259076 Query: 65 -ALENKFSVI--VDDTNYYNSKRRDLMNIAKECDTNYVTIYLKAPLNLLLKRNIE 116 AL ++ VI V + + ++R ++ + K+ D + +++ P+++ R+++ Sbjct: 259077 SALFSEAGVITLVALVSPFRARRDEVRELHKKMDIPFYEVFVDVPVSVAADRDVK 259241 Database: genome.fa Posted date: Jan 19, 2010 5:09 PM Number of letters in database: 78,051,097 Number of sequences in database: 5578 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,193,773 Number of Sequences: 5578 Number of extensions: 104300 Number of successful extensions: 392 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 233 Number of HSP's gapped (non-prelim): 210 length of query: 255 length of database: 26,017,032 effective HSP length: 104 effective length of query: 151 effective length of database: 25,436,920 effective search space: 3840974920 effective search space used: 3840974920 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 63 (28.9 bits)