TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName:
Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec)
kinase; Short=PSTK
         (255 letters)

Database: genome.fa 
           218 sequences; 41,633,360 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_5                                                             30   2.8  

>scaffold_5 
          Length = 1613056

 Score = 29.6 bits (65), Expect = 2.8
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = +2

Query: 221     IRKSFLKDCKKIESEKLDFEKIEKDFLDYLNKNLK 255
               IRK+ +K C  + SE  D E+  K F ++  KNLK
Sbjct: 1535123 IRKNIVKKCLDLFSELSDDEENYKKFYEHFAKNLK 1535227


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:06 PM
  Number of letters in database: 41,633,360
  Number of sequences in database:  218
  
Lambda     K      H
   0.315    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,289,763
Number of Sequences: 218
Number of extensions: 48802
Number of successful extensions: 151
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 142
Number of HSP's gapped (non-prelim): 28
length of query: 255
length of database: 13,877,786
effective HSP length: 100
effective length of query: 155
effective length of database: 13,855,986
effective search space: 2147677830
effective search space used: 2147677830
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 60 (27.7 bits)