TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74553663|sp|Q6LX62.1|PSTK_METMP RecName: Full=L-seryl-tRNA(Sec) kinase; AltName: Full=O-phosphoseryl-tRNA(Sec) kinase; Short=PSTK (255 letters) Database: genome.fa 1696 sequences; 31,706,682 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value mex146m19.p1k 28 4.8 mex450b23.q1k 28 4.8 mex202a13.p1k 28 4.8 >mex146m19.p1k Length = 84414 Score = 28.5 bits (62), Expect = 4.8 Identities = 12/19 (63%), Positives = 14/19 (73%) Frame = -1 Query: 5 LTGLPSVGKSTFSKAFSKK 23 + GLP+VGKSTF SKK Sbjct: 73464 IVGLPNVGKSTFFNVLSKK 73408 >mex450b23.q1k Length = 8702 Score = 28.5 bits (62), Expect = 4.8 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = -1 Query: 2 LIILTGLPSVGKSTFSKAFSKKMAEK 27 +++L GLP GKST + A S+ +A + Sbjct: 3611 VVVLCGLPGAGKSTLAMALSRLLASQ 3534 >mex202a13.p1k Length = 30283 Score = 28.5 bits (62), Expect = 4.8 Identities = 10/28 (35%), Positives = 20/28 (71%) Frame = +3 Query: 1 MLIILTGLPSVGKSTFSKAFSKKMAEKN 28 +L ++TG+P+ GKSTF A + + +++ Sbjct: 13308 LLFLVTGIPAAGKSTFLAAAQRHVVQRD 13391 Database: genome.fa Posted date: Jan 16, 2009 12:51 PM Number of letters in database: 31,706,682 Number of sequences in database: 1696 Lambda K H 0.315 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,231,588 Number of Sequences: 1696 Number of extensions: 31729 Number of successful extensions: 93 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 62 Number of HSP's gapped (non-prelim): 37 length of query: 255 length of database: 10,568,894 effective HSP length: 98 effective length of query: 157 effective length of database: 10,402,686 effective search space: 1633221702 effective search space used: 1633221702 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 59 (27.3 bits)