TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: genome.fa 
           32,746 sequences; 89,612,356 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAHK01002924.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   232   9e-61
gb|AAHK01000776.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   231   2e-60

>gb|AAHK01002924.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053504079, whole genome
            shotgun sequence
          Length = 4296

 Score =  232 bits (592), Expect = 9e-61
 Identities = 155/401 (38%), Positives = 199/401 (49%), Gaps = 13/401 (3%)
 Frame = -2

Query: 41   FEPQTLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSXXXXXXXXXX 100
            F P  +G  P + LT ++ +KG  CK+PQ  LL LL  +                     
Sbjct: 2411 FNPTDVGLPPEFVLTDYTRLKGCSCKLPQPKLLALLRAV--------------------- 2295

Query: 101  XXXXXSTRPGPGSAFPSLSIGMDSCVIPL----RHGG-LSLVQTTDFFYPLVEDPYMMGR 155
                 ST PG         +GMD  V+ L     HG  L +V TTDFF+P VEDPY+ G+
Sbjct: 2294 -----STTPG------QKDVGMDCSVVQLLQKDEHGAPLFMVSTTDFFFPSVEDPYLQGQ 2148

Query: 156  IACANVLSDLYAMGITECDNXXXXXXXXXXXXEKEREKVTPLMIKGFRDXXXXXXXXXXX 215
            IA  NVLSDLY+MGI  CD             E ERE  T  ++KGF D           
Sbjct: 2147 IAATNVLSDLYSMGIDRCDTVLMMLAASTEMDEMEREVSTREIMKGFVDQVRLAGSDVTG 1968

Query: 216  XXXXXNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEK- 274
                 NPW +IGGVAT V  + E + P    +GDVLVLTKPLG Q+A N  QW+  P   
Sbjct: 1967 GQTVMNPWPLIGGVATSVVTEPEMVRPTGLCIGDVLVLTKPLGNQIAVNLKQWVRRPSPI 1788

Query: 275  -WNKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLA 333
                I+  ++ EEVE  Y  A  +M  LNR AA LM    AH ATD+TGFGILGH+ NLA
Sbjct: 1787 YLETIQGKMTTEEVEELYNAATDSMKRLNRNAARLMRRHGAHGATDVTGFGILGHAHNLA 1608

Query: 334  KQQKNEVSFVIHNLPXXXXXXXXXXXXG-RFGLLQGTSAETSGGLLICL-PREQAARFCS 391
              Q+ EV  ++  LP              ++ LL+G SAETSGGLL+       A R+  
Sbjct: 1607 VAQEKEVCLLLEALPIYKGALKASTLMSDKYQLLKGYSAETSGGLLVAFGDLCTAERYIK 1428

Query: 392  EIKSSKYGEGHQAWIVGIV----EKGNRTARIIDKPRVIEV 428
            E+      +   AW+VG V      G+  A++ D  ++IEV
Sbjct: 1427 ELCEV---DREPAWVVGSVIAHCGDGSPHAKLQDGYKIIEV 1314


>gb|AAHK01000776.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053508717, whole genome
             shotgun sequence
          Length = 24108

 Score =  231 bits (589), Expect = 2e-60
 Identities = 156/401 (38%), Positives = 198/401 (49%), Gaps = 13/401 (3%)
 Frame = -1

Query: 41    FEPQTLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSXXXXXXXXXX 100
             F P  +G  P + LT ++ +KG  CK+PQ  LL LL  +                     
Sbjct: 13137 FNPTDVGLPPEFVLTDYTRLKGCSCKLPQPKLLALLRAV--------------------- 13021

Query: 101   XXXXXSTRPGPGSAFPSLSIGMDSCVIPL-----RHGGLSLVQTTDFFYPLVEDPYMMGR 155
                  ST PG         +GMD  V+ L     R   L +V TTDFF+P VEDPY+ G+
Sbjct: 13020 -----STTPG------QKDVGMDCSVVQLLQKNERGAPLFMVSTTDFFFPSVEDPYLQGQ 12874

Query: 156   IACANVLSDLYAMGITECDNXXXXXXXXXXXXEKEREKVTPLMIKGFRDXXXXXXXXXXX 215
             IA +NVLSDLY+MGI  CD             E ERE  T  ++KGF D           
Sbjct: 12873 IAASNVLSDLYSMGIDRCDTVLMMLAASTEMDEVEREISTREIMKGFVDQVRLAGSDVTG 12694

Query: 216   XXXXXNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANAHQWLDNPEK- 274
                  NPW +IGGVAT V  + E + P    +GDVLVLTKPLG QVA N  QW+  P   
Sbjct: 12693 GQTVMNPWPLIGGVATSVVTELEMVRPTGLCIGDVLVLTKPLGNQVAVNLKQWVRRPSPI 12514

Query: 275   -WNKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLA 333
                 I+  ++ EEVE  Y  A  +M  LNR AA LM    AH ATD+TGFGILGH+ NLA
Sbjct: 12513 YLETIQGKMTTEEVEELYNAATDSMKRLNRNAARLMRRHGAHGATDVTGFGILGHAHNLA 12334

Query: 334   KQQKNEVSFVIHNLPXXXXXXXXXXXXG-RFGLLQGTSAETSGGLLICL-PREQAARFCS 391
               Q+ EV  ++  LP              ++ LL+G SAETSGGLL+       A R+  
Sbjct: 12333 VAQEKEVCLLLEALPIYKGALKASKLMSDKYQLLKGYSAETSGGLLVAFGDLCTAERYIK 12154

Query: 392   EIKSSKYGEGHQAWIVGIV----EKGNRTARIIDKPRVIEV 428
             EI      +   AW+VG V      G+  A++ D   +IEV
Sbjct: 12153 EICEV---DREPAWVVGSVIAHCGDGSPHAKLQDGYEIIEV 12040


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:13 PM
  Number of letters in database: 89,612,356
  Number of sequences in database:  32,746
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,956,867
Number of Sequences: 32746
Number of extensions: 659884
Number of successful extensions: 2058
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1923
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2050
length of query: 452
length of database: 29,870,785
effective HSP length: 110
effective length of query: 342
effective length of database: 26,268,725
effective search space: 8983903950
effective search space used: 8983903950
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)