TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: genome.fa 
           1696 sequences; 31,706,682 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

1036_mexFOS1_20a19.q1kpIBR                                            225   6e-59
mex571j02.p1k                                                          29   8.2  

>1036_mexFOS1_20a19.q1kpIBR 
          Length = 7481

 Score =  225 bits (573), Expect = 6e-59
 Identities = 152/410 (37%), Positives = 199/410 (48%), Gaps = 15/410 (3%)
 Frame = +3

Query: 27   AGWSAGRSFSNYRPFEPQTLGFSPSWRLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQP 86
            AG S G +      F+P +LG    ++LT ++ +KG  CK+PQ  LL LL+ L       
Sbjct: 4920 AGKSNGAADLKKPRFDPVSLGLPAEFQLTDYTRLKGCSCKLPQPKLLALLQEL------- 5078

Query: 87   PLTSXXXXXXXXXXXXXXXSTRPGPGSAFPSLSIGMDSCVIPLRHGG-----LSLVQTTD 141
                               ST PG         +G+D  ++PL H       L LV TTD
Sbjct: 5079 -------------------STTPG------QKDVGVDCSIVPLHHTNSKGEALFLVSTTD 5183

Query: 142  FFYPLVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXXXXXEKEREKVTPLMIKG 201
            FF+P V DP++ G+I  ANVLSDLY+MGI +CD             E ER   T  ++KG
Sbjct: 5184 FFFPSVSDPFLQGQIGAANVLSDLYSMGIPDCDTMLMLLAASTEMDEHERLITTREIMKG 5363

Query: 202  FRDXXXXXXXXXXXXXXXXNPWIIIGGVATVVCQQNEFIMPDSAV-VGDVLVLTKPLGTQ 260
            F +                NPW +IGGVA  V  + E + P   +  GD+LVLTKPLG Q
Sbjct: 5364 FAERARLATTTVTGGQTVMNPWPLIGGVAMAVVSEAEMVRPTGLLRAGDILVLTKPLGCQ 5543

Query: 261  VAANAHQWLDNPEKW--NKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAAT 318
            VA N  QWL  P       I   +S EE+E  Y  A  +M  LNR  A LM    AH AT
Sbjct: 5544 VAVNLKQWLLRPSPLYEETIAGHISPEEIEELYNMATDSMRRLNREGARLMRKHGAHGAT 5723

Query: 319  DITGFGILGHSQNLAKQQ-----KNEVSFVIHNLPXXXXXXXXXXXXG-RFGLLQGTSAE 372
            D+TGFGILGH+ N    Q        +  V+  LP              ++ LL+G SAE
Sbjct: 5724 DVTGFGILGHANNFGAAQTVGDAPRSLCLVLERLPMFKTAVAASKRMNDKYRLLEGYSAE 5903

Query: 373  TSGGLLICLPREQAAR-FCSEIKSSKYGEGHQAWIVGIVEKGNRTARIID 421
            TSGGLL+  P   +A  FC+E+  ++   G  +WIVG VE  +R A  +D
Sbjct: 5904 TSGGLLVAFPSTTSAEAFCAEL--TEIDGGCPSWIVGYVE--DRAANALD 6041


>mex571j02.p1k 
          Length = 39356

 Score = 28.9 bits (63), Expect = 8.2
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = +1

Query: 247   VGDVLVLTKPLGTQVAANAHQWLDNPEKWNKIK 279
             VGD  VLT P    V A  HQ +D P + + ++
Sbjct: 17350 VGDTCVLTSPAPLPVGATVHQMVDWPRRMDHMQ 17448


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 31,706,682
  Number of sequences in database:  1696
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,320,667
Number of Sequences: 1696
Number of extensions: 230089
Number of successful extensions: 1016
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 75
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 904
Number of HSP's gapped (non-prelim): 214
length of query: 452
length of database: 10,568,894
effective HSP length: 103
effective length of query: 349
effective length of database: 10,394,206
effective search space: 3627577894
effective search space used: 3627577894
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)