TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000108_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Mus musculus # Complete
         (452 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_457                                                          281   7e-76

>scaffold_457 
          Length = 23069

 Score =  281 bits (719), Expect = 7e-76
 Identities = 169/412 (41%), Positives = 215/412 (52%), Gaps = 34/412 (8%)
 Frame = +1

Query: 53    RLTSFSGMKGUGCKVPQETLLKLLEGLTRPALQPPLTSXXXXXXXXXXXXXXXSTRPGPG 112
             RLT ++ +KG GCKVPQ+ LL LLEG+                              G G
Sbjct: 17437 RLTDYTRLKG*GCKVPQKELLSLLEGI------------------------------GEG 17526

Query: 113   SAFPSLSIGMDSCVIPLRHGGLSLVQTTD---------------------------FFYP 145
                    IG D  +   +   + ++ TTD                           FF+P
Sbjct: 17527 -------IGYDCSITQTKFPDIVMITTTDVRYIKSYSYNKNYKQIYNIFLT*IIF*FFFP 17685

Query: 146   LVEDPYMMGRIACANVLSDLYAMGITECDNXXXXXXXXXXXXEKEREKVTPLMIKGFRDX 205
             LV+DPY  G+IACANVLSDLY+ GI ECDN             ++R+  + L+I+GF D 
Sbjct: 17686 LVDDPYFQGKIACANVLSDLYSFGIEECDNMLMLLACSVDMTPEQRQWSSKLLIQGFNDQ 17865

Query: 206   XXXXXXXXXXXXXXXNPWIIIGGVATVVCQQNEFIMPDSAVVGDVLVLTKPLGTQVAANA 265
                            NPW IIGGVAT + + +EFI P  AV GDVLVLTKPLGTQV  N 
Sbjct: 17866 ATLAGTKVSGGQTVKNPWPIIGGVATSILKTSEFIKPVGAVDGDVLVLTKPLGTQVCVNF 18045

Query: 266   HQWLDNPEKWNKIKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGI 325
             HQWL N ++W KI+ + + +E E  ++ A  +MA LNRT A LM  + AHAATD+TGFGI
Sbjct: 18046 HQWLRNEDRWRKIESITNEQECEEVFEFATKSMARLNRTGARLMKKYGAHAATDVTGFGI 18225

Query: 326   LGHSQNLAKQQKNEVSFVIHNLPXXXXXXXXXXXXGR-FGLLQGTSAETSGGLLICLPRE 384
             LGHS NLA  Q   + F IH LP               F LLQGTSAETSGGLLICLPR+
Sbjct: 18226 LGHSTNLAGNQDASIRFEIHTLPIIKHMKKLEDHLNHPFKLLQGTSAETSGGLLICLPRD 18405

Query: 385   QAARFCSEIKSSKYGEGHQAWIVGIV------EKGNRTARIIDKPRVIEVLP 430
             +A  FC EI+     +   AWI+G V       +   T+ I++ P++IEV P
Sbjct: 18406 KAEAFCKEIEEI---DKQPAWIIGDVIGQSDKPRSMNTSTILENPKIIEVEP 18552


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.319    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,920,332
Number of Sequences: 799
Number of extensions: 113905
Number of successful extensions: 239
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 229
Number of HSP's gapped (non-prelim): 9
length of query: 452
length of database: 10,989,169
effective HSP length: 104
effective length of query: 348
effective length of database: 10,906,073
effective search space: 3795313404
effective search space used: 3795313404
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)