TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: genome.fa 
           32,746 sequences; 89,612,356 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAHK01000776.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   233   7e-61
gb|AAHK01002924.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   231   2e-60
gb|AAHK01006620.1| Trypanosoma cruzi strain CL Brener tcruzi_104...    31   5.3  

>gb|AAHK01000776.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053508717, whole genome
             shotgun sequence
          Length = 24108

 Score =  233 bits (593), Expect = 7e-61
 Identities = 160/412 (38%), Positives = 204/412 (49%), Gaps = 13/412 (3%)
 Frame = -1

Query: 38    FEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEAS 97
             F P  +GL P + LT ++ +KG  CK+PQ  LL LL  ++              GQ++  
Sbjct: 13137 FNPTDVGLPPEFVLTDYTRLKGCSCKLPQPKLLALLRAVSTTP-----------GQKD-- 12997

Query: 98    QEAGLPAGAGPSPTFPALGIGMDSCVIPL-----RHGGLSLVQTTDFFYPLVEDPYMMGR 152
                                +GMD  V+ L     R   L +V TTDFF+P VEDPY+ G+
Sbjct: 12996 -------------------VGMDCSVVQLLQKNERGAPLFMVSTTDFFFPSVEDPYLQGQ 12874

Query: 153   IACANVLSDLYAMGITECDNXXXXXXXXXXXXXXXREKVTPLMVKGFRDXXXXXXXXXXX 212
             IA +NVLSDLY+MGI  CD                RE  T  ++KGF D           
Sbjct: 12873 IAASNVLSDLYSMGIDRCDTVLMMLAASTEMDEVEREISTREIMKGFVDQVRLAGSDVTG 12694

Query: 213   XXXXXNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPER- 271
                  NPW +IGGVAT V    E + P    +GDVLVLTKPLG QVAVN  QW+  P   
Sbjct: 12693 GQTVMNPWPLIGGVATSVVTELEMVRPTGLCIGDVLVLTKPLGNQVAVNLKQWVRRPSPI 12514

Query: 272   -WNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLA 330
                 ++  ++ EEVE  Y  A  +M  LNR AA LM    AH ATD+TGFGILGH+ NLA
Sbjct: 12513 YLETIQGKMTTEEVEELYNAATDSMKRLNRNAARLMRRHGAHGATDVTGFGILGHAHNLA 12334

Query: 331   KQQRNEVSFVIHNLPIIAKMAAVSK-ASGRFGLLQGTSAETSGGLLICL-PREQAARFCS 388
               Q  EV  ++  LPI       SK  S ++ LL+G SAETSGGLL+       A R+  
Sbjct: 12333 VAQEKEVCLLLEALPIYKGALKASKLMSDKYQLLKGYSAETSGGLLVAFGDLCTAERYIK 12154

Query: 389   EIKSSKYGEGHQAWIVGIV----EKGNRTARIIDKPRVIEVLPRGATAAVLA 436
             EI      +   AW+VG V      G+  A++ D   +IEV   G    V A
Sbjct: 12153 EICEV---DREPAWVVGSVIAHCGDGSPHAKLQDGYEIIEV*NNGRFPCVCA 12007


>gb|AAHK01002924.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053504079, whole genome
            shotgun sequence
          Length = 4296

 Score =  231 bits (590), Expect = 2e-60
 Identities = 155/401 (38%), Positives = 203/401 (50%), Gaps = 13/401 (3%)
 Frame = -2

Query: 38   FEPQALGLSPSWRLTGFSGMKGUGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEAS 97
            F P  +GL P + LT ++ +KG  CK+PQ  LL LL  ++              GQ++  
Sbjct: 2411 FNPTDVGLPPEFVLTDYTRLKGCSCKLPQPKLLALLRAVSTTP-----------GQKD-- 2271

Query: 98   QEAGLPAGAGPSPTFPALGIGMDSCVIPL----RHGG-LSLVQTTDFFYPLVEDPYMMGR 152
                               +GMD  V+ L     HG  L +V TTDFF+P VEDPY+ G+
Sbjct: 2270 -------------------VGMDCSVVQLLQKDEHGAPLFMVSTTDFFFPSVEDPYLQGQ 2148

Query: 153  IACANVLSDLYAMGITECDNXXXXXXXXXXXXXXXREKVTPLMVKGFRDXXXXXXXXXXX 212
            IA  NVLSDLY+MGI  CD                RE  T  ++KGF D           
Sbjct: 2147 IAATNVLSDLYSMGIDRCDTVLMMLAASTEMDEMEREVSTREIMKGFVDQVRLAGSDVTG 1968

Query: 213  XXXXXNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPER- 271
                 NPW +IGGVAT V    E + P    +GDVLVLTKPLG Q+AVN  QW+  P   
Sbjct: 1967 GQTVMNPWPLIGGVATSVVTEPEMVRPTGLCIGDVLVLTKPLGNQIAVNLKQWVRRPSPI 1788

Query: 272  -WNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLA 330
                ++  ++ EEVE  Y  A  +M  LNR AA LM    AH ATD+TGFGILGH+ NLA
Sbjct: 1787 YLETIQGKMTTEEVEELYNAATDSMKRLNRNAARLMRRHGAHGATDVTGFGILGHAHNLA 1608

Query: 331  KQQRNEVSFVIHNLPII-AKMAAVSKASGRFGLLQGTSAETSGGLLICL-PREQAARFCS 388
              Q  EV  ++  LPI    + A +  S ++ LL+G SAETSGGLL+       A R+  
Sbjct: 1607 VAQEKEVCLLLEALPIYKGALKASTLMSDKYQLLKGYSAETSGGLLVAFGDLCTAERYIK 1428

Query: 389  EIKSSKYGEGHQAWIVGIV----EKGNRTARIIDKPRVIEV 425
            E+      +   AW+VG V      G+  A++ D  ++IEV
Sbjct: 1427 ELCEV---DREPAWVVGSVIAHCGDGSPHAKLQDGYKIIEV 1314


>gb|AAHK01006620.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053478987, whole
           genome shotgun sequence
          Length = 1420

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 25/81 (30%), Positives = 31/81 (38%), Gaps = 17/81 (20%)
 Frame = +3

Query: 32  FSNYRPFEPQALGLSPSWRLTGFSGM-----------------KGUGCKVPQEALLKLLA 74
           F  Y  F P    L PSW+ + +  M                 KG  C  PQ+ LL  + 
Sbjct: 384 FPLYLFFLPFFFFLQPSWKFSRYLQMSPFSFHFFFSLLFPSPWKGSECAPPQKMLLVPVL 563

Query: 75  GLTRPDVRPPLGRGLVGGQEE 95
           G   P  RPP  RG + G  E
Sbjct: 564 GAYTPRERPP--RGSISGDLE 620


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:13 PM
  Number of letters in database: 89,612,356
  Number of sequences in database:  32,746
  
Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,896,952
Number of Sequences: 32746
Number of extensions: 749630
Number of successful extensions: 2820
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2562
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2812
length of query: 448
length of database: 29,870,785
effective HSP length: 110
effective length of query: 338
effective length of database: 26,268,725
effective search space: 8878829050
effective search space used: 8878829050
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)