TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_36                                                           133   4e-31
scaffold_84                                                            32   1.7  
scaffold_175                                                           30   6.4  
scaffold_70                                                            30   8.3  
scaffold_78                                                            30   8.3  

>scaffold_36
          Length = 507435

 Score =  133 bits (335), Expect = 4e-31
 Identities = 82/222 (36%), Positives = 124/222 (55%), Gaps = 6/222 (2%)
 Frame = -3

Query: 153    IRQLKINPWCII------GGIATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLW 206
              IR + + P CI       GG+  +    ++ I+P+ A+ GD LVLTKPLG Q+A++ + W
Sbjct: 409588 IRLILLGPKCINLFRFIRGGVGIASNHKDDFIMPNGAEAGDYLVLTKPLGVQLAVNTYQW 409409

Query: 207    QLNQTEKYKKLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLLG 266
                    K+  +    ++++I   F+ A KSM  LN  AA L  K+    +TD+TGFG+LG
Sbjct: 409408 MTTIPSKWDAIKGITNESEIN*AFDHATKSMCTLNAAAASLFKKHNIKASTDVTGFGILG 409229

Query: 267    HANNLAQFQKEKVLFQINKLPIIKNVLKFSTLVGQSTKFRSGRSVETSGGLLICLPADAA 326
              HA  LA+  + KV F I+KLPII+N+ K      +  +F  G + ETSGGLLI       
Sbjct: 409228 HARYLARA*RRKV*FVIDKLPIIQNLYKLDK-KARDFRFLEGLAAETSGGLLIA--TKDP 409058

Query: 327    DKFCRDFEEATNGEQKSFQIGHVTAANESDAVLCEDVEFIEV 368
              D F +D++      +  + IG V  + E DAV+ ++V+  EV
Sbjct: 409057 DSFLKDYKHLIG--EWGWVIGRVEKSQERDAVISKNVKIQEV 408938



 Score =  100 bits (248), Expect = 5e-21
 Identities = 55/139 (39%), Positives = 81/139 (58%)
 Frame = -2

Query: 4      PEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPL 63
              PE +GL   F +T FT   G  CK+P   L KYL          +G +     D +V P+
Sbjct: 410570 PENYGLPKGFAMTNFTELKGCGCKVP*T*LLKYLG*I------GEGDIGKETPDVSVYPV 410409

Query: 64     KRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVSTSTSFSE 123
              KR  D+  I T+D+FYP+V +P   GRIA  NVLSD+YA+GVTQ DT+ M++   T  ++
Sbjct: 410408 KRT*DFHTISTIDYFYPLVANPYA*GRIACCNVLSDLYAMGVTQVDTMLMVLGVCTKMTD 410229

Query: 124    KERDVVIGLVMKGFQNSLK 142
              +E+ +   L+++GF +  K
Sbjct: 410228 EEK*ISTSLMLEGFNDCAK 410172


>scaffold_84
          Length = 354111

 Score = 32.0 bits (71), Expect = 1.7
 Identities = 35/133 (26%), Positives = 56/133 (42%), Gaps = 6/133 (4%)
 Frame = +2

Query: 177   IILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADIKETFEIAVKS 236
             ++ P N    D+ +L K     +       Q    E  K+ L   S A+  E FE  +KS
Sbjct: 44228 LVNPRNCVCIDIFILMKSANSNLPNSIQPDQNEINEYIKEYLRYSSFANTLECFEAEIKS 44407

Query: 237   MTYLNKNAALLMHKYQA---HCATDITGFGLLGHANNLA---QFQKEKVLFQINKLPIIK 290
                 NK    L+  ++    + +    G  L  + N L    +  KEK +++ NK P IK
Sbjct: 44408 K*VSNKVFICLILPFRCLINNSSRSK*GMRLQDYFNCLRVTIRKLKEKSIWRRNKRPSIK 44587

Query: 291   NVLKFSTLVGQST 303
             N+ K   L  +S+
Sbjct: 44588 NINKSYRLEDRSS 44626


>scaffold_175
          Length = 139747

 Score = 30.0 bits (66), Expect = 6.4
 Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = -1

Query: 271   LAQFQKEKVLFQINKLPIIKNVLKFSTL-VGQSTKFRSGRSVETSGG 316
             L QF ++ VL +INK+ IIKN   F  L + +  +F+ G  +    G
Sbjct: 28144 LCQFYEKTVL*EINKIVIIKNERLFRMLEINKKERFKKGFRINNKYG 28004


>scaffold_70
          Length = 388433

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 18/41 (43%), Positives = 22/41 (53%)
 Frame = -1

Query: 220   ECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDIT 260
             +C + D  +   I VK    LNK+A LL   YQA  A DIT
Sbjct: 21944 KCRNQDCGDNGIIEVKIDKTLNKDALLLKKDYQAAKAIDIT 21822


>scaffold_78
          Length = 358839

 Score = 29.6 bits (65), Expect = 8.3
 Identities = 18/41 (43%), Positives = 22/41 (53%)
 Frame = -3

Query: 220   ECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDIT 260
             +C + D  +   I VK    LNK+A LL   YQA  A DIT
Sbjct: 21196 KCRNQDCGDNGIIEVKIDKTLNKDALLLKKDYQAAKAIDIT 21074


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,967,756
Number of Sequences: 697
Number of extensions: 336167
Number of successful extensions: 1145
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1084
Number of HSP's gapped (non-prelim): 148
length of query: 370
length of database: 23,960,378
effective HSP length: 107
effective length of query: 263
effective length of database: 23,885,799
effective search space: 6281965137
effective search space used: 6281965137
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)