TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: genome.fa 
           1696 sequences; 31,706,682 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

1036_mexFOS1_20a19.q1kpIBR                                            225   5e-59
mex365k04.q1k                                                          33   0.44 
mex141e04.q1k                                                          30   3.7  

>1036_mexFOS1_20a19.q1kpIBR 
          Length = 7481

 Score =  225 bits (573), Expect = 5e-59
 Identities = 141/384 (36%), Positives = 210/384 (54%), Gaps = 17/384 (4%)
 Frame = +3

Query: 2    FQPEKHGLEPDFQLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVI 61
            F P   GL  +FQLT +T   G SCK+PQ  L   L+         D      G+DC+++
Sbjct: 4962 FDPVSLGLPAEFQLTDYTRLKGCSCKLPQPKLLALLQELSTTPGQKD-----VGVDCSIV 5126

Query: 62   PL----KRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIVST 117
            PL     + +   L+ T DFF+P V+DP L G+I  ANVLSD+Y++G+   DT+ M+++ 
Sbjct: 5127 PLHHTNSKGEALFLVSTTDFFFPSVSDPFLQGQIGAANVLSDLYSMGIPDCDTMLMLLAA 5306

Query: 118  STSFSEKERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSEEI 177
            ST   E ER +    +MKGF    +      T +   Q  +NPW +IGG+A +V    E+
Sbjct: 5307 STEMDEHERLITTREIMKGFAERAR---LATTTVTGGQTVMNPWPLIGGVAMAVVSEAEM 5477

Query: 178  ILPSN-AQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLS-ECSDADIKETFEIAVK 235
            + P+   + GD+LVLTKPLG Q+A++   W L  +  Y++ ++   S  +I+E + +A  
Sbjct: 5478 VRPTGLLRAGDILVLTKPLGCQVAVNLKQWLLRPSPLYEETIAGHISPEEIEELYNMATD 5657

Query: 236  SMTYLNKNAALLMHKYQAHCATDITGFGLLGHANNLAQFQ-----KEKVLFQINKLPIIK 290
            SM  LN+  A LM K+ AH ATD+TGFG+LGHANN    Q        +   + +LP+ K
Sbjct: 5658 SMRRLNREGARLMRKHGAHGATDVTGFGILGHANNFGAAQTVGDAPRSLCLVLERLPMFK 5837

Query: 291  NVLKFSTLVGQSTKFRSGRSVETSGGLLICLPA-DAADKFCRDFEEATNGEQKSFQIGHV 349
              +  S  +    +   G S ETSGGLL+  P+  +A+ FC +  E  +G   S+ +G+V
Sbjct: 5838 TAVAASKRMNDKYRLLEGYSAETSGGLLVAFPSTTSAEAFCAELTE-IDGGCPSWIVGYV 6014

Query: 350  --TAANESDAV---LCEDVEFIEV 368
               AAN  D V   L +  E +EV
Sbjct: 6015 EDRAANALDGVYARLKDGCEIVEV 6086


>mex365k04.q1k 
          Length = 59388

 Score = 32.7 bits (73), Expect = 0.44
 Identities = 21/63 (33%), Positives = 32/63 (50%)
 Frame = -1

Query: 293   LKFSTLVGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVTAA 352
             L FST+     +    R V T G  L+ L +    + CRD  E  +G  +S ++G V+AA
Sbjct: 53547 LSFSTVSNGKPRMAWRRGVSTCGVRLLKLRSSTRSRVCRDTTERKDG--RSGRLGDVSAA 53374

Query: 353   NES 355
             + S
Sbjct: 53373 SPS 53365


>mex141e04.q1k 
          Length = 11915

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 14/32 (43%), Positives = 15/32 (46%)
 Frame = -1

Query: 301   QSTKFRSGRSVETSGGLLICLPADAADKFCRD 332
             + T   SGR     GGL   LP   AD FC D
Sbjct: 10658 EHTHRESGRQTRVHGGLAASLPLTEADDFCPD 10563


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 31,706,682
  Number of sequences in database:  1696
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,195,692
Number of Sequences: 1696
Number of extensions: 165664
Number of successful extensions: 623
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 543
Number of HSP's gapped (non-prelim): 139
length of query: 370
length of database: 10,568,894
effective HSP length: 102
effective length of query: 268
effective length of database: 10,395,902
effective search space: 2786101736
effective search space used: 2786101736
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)