TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000170.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   5.4  
gb|AAFB02000009.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   5.4  
gb|AAFB02000193.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   9.2  

>gb|AAFB02000170.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750511690, whole genome
             shotgun sequence
          Length = 32659

 Score = 28.5 bits (62), Expect = 5.4
 Identities = 18/63 (28%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
 Frame = +1

Query: 130   IGLVMKGFQNSLKANGYRNTPLIIRQLKINPW-CIIGGIATSVCRSEE--IILPSNAQPG 186
             I L ++G  N    N Y   P+I   L   PW C    I + +   +   +++PS+  P 
Sbjct: 14923 ISLNIRGIDNPYHVNIYPKEPIIPMVLSSPPWNCFSPQIQSDIPLPQNLTLVMPSSLSPN 15102

Query: 187   DVL 189
              VL
Sbjct: 15103 SVL 15111


>gb|AAFB02000009.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750506659, whole genome
             shotgun sequence
          Length = 116940

 Score = 28.5 bits (62), Expect = 5.4
 Identities = 22/84 (26%), Positives = 39/84 (46%), Gaps = 1/84 (1%)
 Frame = -3

Query: 204   HLWQLNQTEKYK-KLLSECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGF 262
             H+  L +TE     LL    +  +++ ++  +   T+L+ N  +L  +Y A    +    
Sbjct: 66643 HIKSLIKTESLDLSLLRNIDELIVEKVYQSQITLPTFLS-NLIILKSRYFALTNAESI-- 66473

Query: 263   GLLGHANNLAQFQKEKVLFQINKL 286
                 H +NLA  Q E + F INK+
Sbjct: 66472 ----HLHNLALLQSELIFFDINKV 66413


>gb|AAFB02000193.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512870, whole genome
             shotgun sequence
          Length = 30017

 Score = 27.7 bits (60), Expect = 9.2
 Identities = 21/73 (28%), Positives = 30/73 (41%)
 Frame = -1

Query: 296   STLVGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQIGHVTAANES 355
             ST  G+         V  SGGL + L + +       +  + NGE K  + G     NE 
Sbjct: 26633 STFYGEERPVIINGKVLISGGLTLPLLSSS-------YLVSKNGEIKRLKTGQSFEENEE 26475

Query: 356   DAVLCEDVEFIEV 368
             D +LC   E I +
Sbjct: 26474 DQLLCVCYELITI 26436


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,325,833
Number of Sequences: 2172
Number of extensions: 102149
Number of successful extensions: 385
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 254
Number of HSP's gapped (non-prelim): 194
length of query: 370
length of database: 6,923,698
effective HSP length: 98
effective length of query: 272
effective length of database: 6,710,842
effective search space: 1825349024
effective search space used: 1825349024
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)