TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Drosophila melanogaster # Complete
         (370 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_457                                                          237   1e-62

>scaffold_457 
          Length = 23069

 Score =  237 bits (604), Expect = 1e-62
 Identities = 145/387 (37%), Positives = 212/387 (54%), Gaps = 32/387 (8%)
 Frame = +1

Query: 14    QLTKFTTHTGUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHKDYLLIQ 73
             +LT +T   G  CK+PQK L   L G             G G DC++   K   D ++I 
Sbjct: 17437 RLTDYTRLKG*GCKVPQKELLSLLEGIGE----------GIGYDCSITQTK-FPDIVMIT 17583

Query: 74    TVD---------------------------FFYPMVNDPELLGRIALANVLSDVYAVGVT 106
             T D                           FF+P+V+DP   G+IA ANVLSD+Y+ G+ 
Sbjct: 17584 TTDVRYIKSYSYNKNYKQIYNIFLT*IIF*FFFPLVDDPYFQGKIACANVLSDLYSFGIE 17763

Query: 107   QFDTVEMIVSTSTSFSEKERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGG 166
             + D + M+++ S   + ++R     L+++GF +     G   T +   Q   NPW IIGG
Sbjct: 17764 ECDNMLMLLACSVDMTPEQRQWSSKLLIQGFNDQATLAG---TKVSGGQTVKNPWPIIGG 17934

Query: 167   IATSVCRSEEIILPSNAQPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYKKLLSECSDADI 226
             +ATS+ ++ E I P  A  GDVLVLTKPLG Q+ ++ H W  N+ ++++K+ S  ++ + 
Sbjct: 17935 VATSILKTSEFIKPVGAVDGDVLVLTKPLGTQVCVNFHQWLRNE-DRWRKIESITNEQEC 18111

Query: 227   KETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLLGHANNLAQFQKEKVLFQINKL 286
             +E FE A KSM  LN+  A LM KY AH ATD+TGFG+LGH+ NLA  Q   + F+I+ L
Sbjct: 18112 EEVFEFATKSMARLNRTGARLMKKYGAHAATDVTGFGILGHSTNLAGNQDASIRFEIHTL 18291

Query: 287   PIIKNVLKFSTLVGQSTKFRSGRSVETSGGLLICLPADAADKFCRDFEEATNGEQKSFQI 346
             PIIK++ K    +    K   G S ETSGGLLICLP D A+ FC++ EE    +Q ++ I
Sbjct: 18292 PIIKHMKKLEDHLNHPFKLLQGTSAETSGGLLICLPRDKAEAFCKEIEEI--DKQPAWII 18465

Query: 347   GHVTAANE-----SDAVLCEDVEFIEV 368
             G V   ++     + + + E+ + IEV
Sbjct: 18466 GDVIGQSDKPRSMNTSTILENPKIIEV 18546


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,298,944
Number of Sequences: 799
Number of extensions: 178567
Number of successful extensions: 655
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 613
Number of HSP's gapped (non-prelim): 83
length of query: 370
length of database: 10,989,169
effective HSP length: 102
effective length of query: 268
effective length of database: 10,907,671
effective search space: 2923255828
effective search space used: 2923255828
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)