TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000117.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    31   1.1  
gb|AAFB02000353.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   5.4  

>gb|AAFB02000117.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514603, whole genome
             shotgun sequence
          Length = 40961

 Score = 30.8 bits (68), Expect = 1.1
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = -1

Query: 111   TEIDQIKLIVTAPTEFTEQEREVVVPMVMQGFLEAAKACNAP 152
             T+ D+ K+IVT P  FT+Q+++  +     G L+  +    P
Sbjct: 29873 TQTDKFKVIVTVPATFTDQQKDATLCAAQLGGLDVIQILPEP 29748


>gb|AAFB02000353.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750508350, whole genome
            shotgun sequence
          Length = 16559

 Score = 28.5 bits (62), Expect = 5.4
 Identities = 11/31 (35%), Positives = 21/31 (67%)
 Frame = -3

Query: 93   LGKIALANVVSDVFAVGATEIDQIKLIVTAP 123
            +GK++   V SDV+++G   I++ + +VT P
Sbjct: 3450 IGKLSTGRVESDVYSIGHQNIEKEQSVVTKP 3358


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,720,900
Number of Sequences: 2172
Number of extensions: 56848
Number of successful extensions: 171
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 54
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 114
Number of HSP's gapped (non-prelim): 73
length of query: 369
length of database: 6,923,698
effective HSP length: 98
effective length of query: 271
effective length of database: 6,710,842
effective search space: 1818638182
effective search space used: 1818638182
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)