TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Anopheles gambiae # Complete
         (369 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_457                                                          270   1e-72
scaffold_87                                                            28   8.6  

>scaffold_457 
          Length = 23069

 Score =  270 bits (690), Expect = 1e-72
 Identities = 146/365 (40%), Positives = 206/365 (56%), Gaps = 28/365 (7%)
 Frame = +1

Query: 14    RLTKFSTLRGUGSKVPQDVLNRLLAGVYGEQLGDKDGKGPNKEEGVGIGLDSSVIALKH- 72
             RLT ++ L+G G KVPQ  L  LL G+                 G GIG D S+   K  
Sbjct: 17437 RLTDYTRLKG*GCKVPQKELLSLLEGI-----------------GEGIGYDCSITQTKFP 17565

Query: 73    DLFLVQSVD---------------------------FFYPLIDDPFMLGKIALANVVSDV 105
             D+ ++ + D                           FF+PL+DDP+  GKIA ANV+SD+
Sbjct: 17566 DIVMITTTDVRYIKSYSYNKNYKQIYNIFLT*IIF*FFFPLVDDPYFQGKIACANVLSDL 17745

Query: 106   FAVGATEIDQIKLIVTAPTEFTEQEREVVVPMVMQGFLEAAKACNAPVQIGSIAENPWCV 165
             ++ G  E D + +++    + T ++R+    +++QGF + A      V  G   +NPW +
Sbjct: 17746 YSFGIEECDNMLMLLACSVDMTPEQRQWSSKLLIQGFNDQATLAGTKVSGGQTVKNPWPI 17925

Query: 166   IGGAASAVCHRSELIMPYNAQPGDALVLTKPLGTQLATNAYIWMGEQQSDSWAQLRERFT 225
             IGG A+++   SE I P  A  GD LVLTKPLGTQ+  N + W+  +  D W ++     
Sbjct: 17926 IGGVATSILKTSEFIKPVGAVDGDVLVLTKPLGTQVCVNFHQWL--RNEDRWRKIESITN 18099

Query: 226   VADIEQTYRIALESMSRLNKTGAELMKKYGAHAATDVTGFGLYGHAENLASHQTADVDFH 285
               + E+ +  A +SM+RLN+TGA LMKKYGAHAATDVTGFG+ GH+ NLA +Q A + F 
Sbjct: 18100 EQECEEVFEFATKSMARLNRTGARLMKKYGAHAATDVTGFGILGHSTNLAGNQDASIRFE 18279

Query: 286   LDTLPIIKNVREIAETLGRGAKLLAGKAVETSGGLLICLPREAAAGFCEEYRRCTKHEAW 345
             + TLPIIK+++++ + L    KLL G + ETSGGLLICLPR+ A  FC+E     K  AW
Sbjct: 18280 IHTLPIIKHMKKLEDHLNHPFKLLQGTSAETSGGLLICLPRDKAEAFCKEIEEIDKQPAW 18459

Query: 346   IVGHV 350
             I+G V
Sbjct: 18460 IIGDV 18474


>scaffold_87 
          Length = 87763

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 29/140 (20%), Positives = 59/140 (42%), Gaps = 1/140 (0%)
 Frame = -3

Query: 16    TKFSTLRGUGSKVPQDVLNRLLAGVYGEQLGDKDGKGPNKEEGVGIGLDSSV-IALKHDL 74
             T  +T  G    V  D LN L +GV G  +    G      E     +DS   +++++  
Sbjct: 11747 THINTPEGDFDVVGSDSLNILESGVIGAFI---QGLEDEFYEVRSSAIDSMCELSVRNSE 11577

Query: 75    FLVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQIKLIVTAPTEFTEQEREVV 134
             F  +++DF   + +D     +I   N +  +      + DQ+ +I+      + +ER+ +
Sbjct: 11576 FAQKNIDFLVDIFNDEIESVRINSINSLRKISNNVVIKEDQLHIILANLESTSREERQSL 11397

Query: 135   VPMVMQGFLEAAKACNAPVQ 154
               ++   +L      +A +Q
Sbjct: 11396 HKLLANIYLSNYSCLSATIQ 11337


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.319    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,069,160
Number of Sequences: 799
Number of extensions: 109957
Number of successful extensions: 311
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 294
Number of HSP's gapped (non-prelim): 23
length of query: 369
length of database: 10,989,169
effective HSP length: 102
effective length of query: 267
effective length of database: 10,907,671
effective search space: 2912348157
effective search space used: 2912348157
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)