TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000106_1.0 # Protein # SECIS binding protein 2 (SBP2) # Mus musculus # Complete (858 letters) Database: genome.fa 697 sequences; 71,881,135 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value scaffold_112 31 8.1 >scaffold_112 Length = 297279 Score = 31.2 bits (69), Expect = 8.1 Identities = 18/63 (28%), Positives = 35/63 (55%), Gaps = 2/63 (3%) Frame = -3 Query: 711 GLDDTLHTIIDCACEQNIPFVFALNRKALGRSLN-KAVP-VSIVGIFSYDGAQDQFHKMV 768 G D + ++ +NIP +FA R LGR+ + K P +S++ + +Y ++ F K++ Sbjct: 164458 GPDHQILKVLKECFNKNIPVIFASTRSKLGRAFSGKFGPKISVISVINY*NMEE*FTKLI 164279 Query: 769 ELT 771 L+ Sbjct: 164278 *LS 164270 Database: genome.fa Posted date: Feb 10, 2010 10:01 AM Number of letters in database: 71,881,135 Number of sequences in database: 697 Lambda K H 0.311 0.126 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 42,349,816 Number of Sequences: 697 Number of extensions: 444131 Number of successful extensions: 988 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 951 Number of HSP's gapped (non-prelim): 104 length of query: 858 length of database: 23,960,378 effective HSP length: 115 effective length of query: 743 effective length of database: 23,880,223 effective search space: 17743005689 effective search space used: 17743005689 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 68 (30.8 bits)