TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000106_1.0 # Protein # SECIS binding protein 2 (SBP2) #
Mus musculus # Complete
         (858 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000362.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    32   1.3  
gb|AAFB02000031.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    32   1.8  
gb|AAFB02000021.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    31   2.3  
gb|AAFB02001114.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    30   3.9  
gb|AAFB02000161.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    30   6.7  

>gb|AAFB02000362.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514585, whole genome
            shotgun sequence
          Length = 16311

 Score = 32.0 bits (71), Expect = 1.3
 Identities = 29/107 (27%), Positives = 47/107 (43%)
 Frame = +2

Query: 653  LLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGL 712
            L  +L+ F  +  Q   VK         G  E  K L   K   +II+ + +       +
Sbjct: 5750 LQNKLIEFVQQAQQNGFVKK--------GANEATKALNRGKADIVIIAADTKP------I 5887

Query: 713  DDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDG 759
            +  LH  I C  ++N+P+VF  ++ ALGR+   +  V  V I +  G
Sbjct: 5888 EIVLHLPILCE-DKNVPYVFVGSKAALGRACGVSRDVIAVAIINDKG 6025


>gb|AAFB02000031.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514745, whole genome
             shotgun sequence
          Length = 71795

 Score = 31.6 bits (70), Expect = 1.8
 Identities = 14/43 (32%), Positives = 24/43 (55%)
 Frame = -1

Query: 567   DVESGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTE 609
             + E GA N+  ++++ TG  K +E   +    EGE++   GTE
Sbjct: 38678 NTEGGAQNEATTKNSETGESKKQEGSGNGQTSEGENKAIKGTE 38550


>gb|AAFB02000021.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750513524, whole genome
             shotgun sequence
          Length = 81723

 Score = 31.2 bits (69), Expect = 2.3
 Identities = 28/107 (26%), Positives = 47/107 (43%)
 Frame = -1

Query: 653   LLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSKGGL 712
             L  +L+ F  +  Q   VK         G  E  K L   K   +I++ + +       +
Sbjct: 61488 LQNKLIEFVQQAQQNGFVKK--------GANETTKALNRGKADIVIMAADTQP------I 61351

Query: 713   DDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDG 759
             +  LH  I C  ++N+P+VF  ++ ALGR+   +  V  V I +  G
Sbjct: 61350 EIVLHLPILCE-DKNVPYVFVGSKAALGRACGVSRDVIAVAIINDKG 61213


>gb|AAFB02001114.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750508908, whole genome
           shotgun sequence
          Length = 2803

 Score = 30.4 bits (67), Expect = 3.9
 Identities = 14/54 (25%), Positives = 28/54 (51%)
 Frame = +2

Query: 570 SGASNQTPSQDNPTGPEKTEESVSSTPVVEGESEEPAGTEFQRDPEACQPAPDS 623
           S  ++ TP+ + P+ P+    S +STP  + E+  P   + + + +  QP+  S
Sbjct: 497 SPQTSSTPTTNQPSQPQTNSASSTSTPQSQPENNNPQPPQTKPEEQPQQPSSQS 658


>gb|AAFB02000161.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750504745, whole genome
            shotgun sequence
          Length = 33275

 Score = 29.6 bits (65), Expect = 6.7
 Identities = 24/89 (26%), Positives = 36/89 (40%), Gaps = 4/89 (4%)
 Frame = +2

Query: 272  MVKSDHTDGAVTSNATTSSPSCTQELSWTPMGYIVRQTV----SSDSAAATENVTSMINL 327
            +V S+ T G +  NAT  S    +E+  TP+    + T+    SS      +   +    
Sbjct: 2303 VVNSNGTTGRLRVNATVQSKQQEEEIINTPLSVQQKPTIPPIKSSKKGNEIDTDKNPTTG 2482

Query: 328  KKTTSSADAKNVSVTSEALSSNPSYNREK 356
              TTS  D K  S   E  ++ P  N  K
Sbjct: 2483 LTTTSKEDRKKSSEKKEIKTTTPQENTVK 2569


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.311    0.126    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,160,311
Number of Sequences: 2172
Number of extensions: 113775
Number of successful extensions: 360
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 48
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 293
Number of HSP's gapped (non-prelim): 111
length of query: 858
length of database: 6,923,698
effective HSP length: 106
effective length of query: 752
effective length of database: 6,693,466
effective search space: 5033486432
effective search space used: 5033486432
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 64 (29.3 bits)