TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) #
Homo sapiens # Complete
         (854 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000148.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    37   0.041
gb|AAFB02000584.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    35   0.16 
gb|AAFB02000453.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    31   3.0  
gb|AAFB02000240.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    30   6.6  

>gb|AAFB02000148.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750511372, whole genome
             shotgun sequence
          Length = 35530

 Score = 37.0 bits (84), Expect = 0.041
 Identities = 34/180 (18%), Positives = 74/180 (41%)
 Frame = -1

Query: 493   ERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAV 552
             E+ ++   ++   N       + K+ +Q++I K +KP  + K   K  +   +R+++  V
Sbjct: 20710 EKRKKPFNVENLKNKKRKRKKIKKEKQQKKI*KRRKPFRI*KGFPKILKSTGERMEDIPV 20531

Query: 553   SPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEAS 612
             SP  T++          ++ P    +      D + S  S   K  E    +++R     
Sbjct: 20530 SPIHTNESF-------PNKLPPATSIISAISEDRIESEESQIIKKSESDNKKIRRKNNV- 20375

Query: 613   HLAPNHTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRR 672
                 N  T   IH+   R Y ++ ++   +   + + K + R  D   Q+D + +  ++R
Sbjct: 20374 ----NEETLKIIHNMYIRGYKAKEIAIITELTKSTVYKYIERMNDENPQRDDMSSLIRKR 20207


>gb|AAFB02000584.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505767, whole genome
            shotgun sequence
          Length = 8361

 Score = 35.0 bits (79), Expect = 0.16
 Identities = 36/168 (21%), Positives = 68/168 (40%), Gaps = 11/168 (6%)
 Frame = +3

Query: 394  VLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQPQDNFKNNVK------KSQLP 447
            VL+   P  I   +  P   V S+ ++  + P+++ KQ+  +  K  ++      K +  
Sbjct: 3453 VLSKAPPSHIVSTKTEPKTVVTSQNKNTGQEPEWKRKQREAEEKKKEMQDKLKAIKQKRL 3632

Query: 448  VQLDLGGMLTALEKKQHSQHAKQXXXXXXXXXXXXXXXXXECASGERGRRMSQMKTPHNP 507
             ++     +T+ + K  S  +KQ                 +    E+ R+  ++K     
Sbjct: 3633 EEVKKKNQITSEKSKTASLSSKQKEPEEIETKKQELDDQAKKIEEEKQRKEKELKLQQE- 3809

Query: 508  LDSSAPLMKKGKQREIPKAKKPTSLKKII-----LKERQERKQRLQEN 550
                    ++ K+REI K K+    KK        KER+E  +RL+EN
Sbjct: 3810 --------EEKKKREIEKTKQEEEKKKRDEEFKQQKEREENLKRLEEN 3929


>gb|AAFB02000453.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512154, whole genome
            shotgun sequence
          Length = 12212

 Score = 30.8 bits (68), Expect = 3.0
 Identities = 27/109 (24%), Positives = 47/109 (43%), Gaps = 8/109 (7%)
 Frame = -3

Query: 519  KQREIPKAKKPTSLKKIILKERQERKQRLQE--NAVSPAFTSDDTQDGESGGDDQFPEQA 576
            K+ EIP+    +S KK  LKE  +  Q  Q+  N        D  +  E    DQ  ++A
Sbjct: 2454 KKEEIPEKGSKSSEKKESLKENNQENQTQQQELNEYEDETEKDKKKTQEESESDQKQDKA 2275

Query: 577  ELSGPEG-----MDELISTPSVEDKSEEPPGTELQR-DTEASHLAPNHT 619
              +  +G      D  + T + +  S E    +++  DTE+++    +T
Sbjct: 2274 STNQSKGTTSEKKDSKVGTNNKDGTSPEAQNDKVKNPDTESANKKNENT 2128


>gb|AAFB02000240.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512110, whole genome
            shotgun sequence
          Length = 24896

 Score = 29.6 bits (65), Expect = 6.6
 Identities = 24/84 (28%), Positives = 42/84 (50%), Gaps = 7/84 (8%)
 Frame = +3

Query: 282  ANAATNSPSCTR-----ELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKN 336
            + A   +PS  R     E+S+TP  Y    T          N+T  +N +   S +  K+
Sbjct: 4068 SRANQTAPSSPRKLKNIEVSYTPSSYKTENTFI--------NIT-YLNYRKEDSRSRSKS 4220

Query: 337  VSIPSSEALS--SDPSYNKEKHII 358
             S+ +S+++S  + PS+NKE+ +I
Sbjct: 4221 HSLSNSKSVSRSTSPSFNKEREVI 4292


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.311    0.128    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,596,007
Number of Sequences: 2172
Number of extensions: 135828
Number of successful extensions: 447
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 50
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 372
Number of HSP's gapped (non-prelim): 141
length of query: 854
length of database: 6,923,698
effective HSP length: 106
effective length of query: 748
effective length of database: 6,693,466
effective search space: 5006712568
effective search space used: 5006712568
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 64 (29.3 bits)