TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000087_1.0 # Protein # SECIS binding protein 2 (SBP2) #
Drosophila melanogaster # Complete
         (313 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_50                                                            32   1.7  
scaffold_72                                                            31   2.9  
scaffold_90                                                            30   3.8  

>scaffold_50
          Length = 463635

 Score = 31.6 bits (70), Expect = 1.7
 Identities = 27/113 (23%), Positives = 57/113 (50%), Gaps = 2/113 (1%)
 Frame = -2

Query: 3      EKIRR--SRNQDVQDTAIKITPRSSKYKNQHRKREQQTSLLDFVIKPRPKTQRQTKAHKL 60
              +KI R  S+N +     +K+  R+   K ++ K++Q+ SL    +  + K +   + +KL
Sbjct: 356810 KKIMRLGSKNIN*MKKKLKLA*RN*NKKRKNTKKDQKKSLRLLKMMRKAKMKMNKEFNKL 356631

Query: 61     QKTHLAITRGSYIVYKPKGKTRLDPKKKITRLKKSVRVYRTSKKAEREVAEND 113
              +   + I R +      K + +L  +KK  + K   ++ +  K+ +RE+ +ND
Sbjct: 356630 K---IKIKRRT-----KKRQKKLRKRKKKKKKKNKNKIRKKKKRIKREIKKND 356496


>scaffold_72
          Length = 386812

 Score = 30.8 bits (68), Expect = 2.9
 Identities = 17/39 (43%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
 Frame = +3

Query: 61     QKTHLAIT-RGSYIVYKPKGKTRLDPKKKITRLKKSVRV 98
              QK H A+   G Y+VY  K K   D K K T  KK ++V
Sbjct: 100527 QKRHFAVRDNGKYLVYSKKKKLSKDVKPKGTFFKKFIKV 100643


>scaffold_90
          Length = 339195

 Score = 30.4 bits (67), Expect = 3.8
 Identities = 17/44 (38%), Positives = 24/44 (54%)
 Frame = -2

Query: 222    KVKLLFLATDCEICPGESGLDATIEGLKFQCQQQQVPYCFPLLR 265
              K+KLL LA      P  S +   +E    +C Q+QVP+CF  L+
Sbjct: 307256 KLKLLILAY-----PNSSLIKIKMESSFMKCGQEQVPFCFKPLK 307140


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.319    0.132    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,786,074
Number of Sequences: 697
Number of extensions: 218098
Number of successful extensions: 751
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 725
Number of HSP's gapped (non-prelim): 87
length of query: 313
length of database: 23,960,378
effective HSP length: 106
effective length of query: 207
effective length of database: 23,886,496
effective search space: 4944504672
effective search space used: 4944504672
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 64 (29.3 bits)