TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|70879148|gb|EAN92345.1| hypothetical protein, conserved [Trypanosoma cruzi] (386 letters) Database: genome.fa 2172 sequences; 20,771,095 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAFB02000236.1| Entamoeba histolytica HM-1:IMSS gcontig_11047... 29 3.3 >gb|AAFB02000236.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505009, whole genome shotgun sequence Length = 24989 Score = 29.3 bits (64), Expect = 3.3 Identities = 18/60 (30%), Positives = 32/60 (53%) Frame = -2 Query: 6 VLLSGLPGAGKTTLSLAIQRLSEQVTTEGERSNSRHGGVVEAVLELDTFISSYEERDGTQ 65 VLL+G PG GKTT+S + + E S++R+ +E ++ F++ DG++ Sbjct: 14530 VLLAGAPGVGKTTVSKIVGKTLGFNPIEFNASDTRNKSSIELAIK-RIFLNGQISIDGSK 14354 Database: genome.fa Posted date: Jan 19, 2010 5:04 PM Number of letters in database: 20,771,095 Number of sequences in database: 2172 Lambda K H 0.319 0.133 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,482,652 Number of Sequences: 2172 Number of extensions: 75113 Number of successful extensions: 255 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 72 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 178 Number of HSP's gapped (non-prelim): 100 length of query: 386 length of database: 6,923,698 effective HSP length: 99 effective length of query: 287 effective length of database: 6,708,670 effective search space: 1925388290 effective search space used: 1925388290 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.7 bits)