TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|27369676|ref|NP_766078.1| O-phosphoseryl-tRNA(Sec) selenium
transferase [Mus musculus]
         (504 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_7                                                            349   3e-98
scaffold_6                                                             32   3.3  
scaffold_158                                                           30   9.5  

>scaffold_7
          Length = 741107

 Score =  349 bits (895), Expect(2) = 3e-98
 Identities = 185/421 (43%), Positives = 275/421 (65%), Gaps = 5/421 (1%)
 Frame = -3

Query: 13     VSPAYVRQGCEARRAHEHLIRLLLEQGKCPEDGWDESTLELFLHELAVMDSNNFLGNCGV 72
              +   YV+    +  A E L +LLL   + PE+G+D+ T+E  L+  A MD+NNF    GV
Sbjct: 284109 IDDKYVKIASNSLNAKEKLFKLLLS*RQLPEEGFDDLTIEYLLNIFATMDTNNFSNKVGV 283930

Query: 73     GEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLNVIKLAGVH 132
              GERE R+ SALV +R+Y   HGIGRSGD+ A QPKAAGSSL+ K+T  L  + +KL+  +
Sbjct: 283929 GERESRIFSALV*KRNYYMGHGIGRSGDLIASQPKAAGSSLIAKLTKHLAKDALKLSN-Y 283753

Query: 133    SVASCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMVTAGFEPVVIENV 192
               +    +VP+ATGM+++LC L L+  +P AKY++WPRID K+C K + TA   P+VI++V
Sbjct: 283752 QINELLLVPVATGMAISLCLLALKSLKP*AKYVLWPRID*KTCLKCI*TANLIPIVIDSV 283573

Query: 193    LEGDELRTDLKAVEAKIQELGPEHILCLHSTTACFAPRVPDRLEELAVICANYDIPHVVN 252
              ++   + T+L  +  K+ E+GP++I+C+ STT+CFAPR+PD + E+A IC  + I HVVN
Sbjct: 283572 IQDHVVTTNLDMLI*KVNEIGPDNIVCVLSTTSCFAPRIPDNIGEIAKICK*FSIYHVVN 283393

Query: 253    NAYGLQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNEPFIQDISKMYPG 312
              NAYGL  +K  + I  G   G ++  V S DKNFMVPVGGA+I G ++  I+ IS++YPG
Sbjct: 283392 NAYGL*CNKIANSINLGLA*GTVNLIVSSTDKNFMVPVGGALIYGNDKKLIK*ISELYPG 283213

Query: 313    RASASPSLDVLITLLSLGCSGYRKLLKERKEMFVYLSTQLKKLAEAHNERLLQTPHNPIS 372
              RASA+P LD+ IT LS+G SGY +L KER E + Y   +L  +    +E++L TP N IS
Sbjct: 283212 RASAAPILDLFITFLSMGKSGYLQLQKERVENYAYFKEKL*IMT*KISEQILNTPKNSIS 283033

Query: 373    LAMTLKTI-----DGHHDKAVTQLGSMLFTRQVSGARAVPLGNVQTVSGHTFRGFMSHAD 427
              + ++L  +     +      +T+LG++L++++V G+R +     + V G  F+ + SH  
Sbjct: 283032 IGISL*NLKID*NN*EEKN*ITKLGAILYSKKVMGSRVITNKKNKVVCGIEFKNYGSHCG 282853

Query: 428    N 428
              N
Sbjct: 282852 N 282850



 Score = 30.4 bits (67), Expect(2) = 3e-98
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = -1

Query: 427    DNYPCAYLNAAAAIGMKMQDVDLFIKRLDK 456
              DNY   Y+  A AIG+  Q++D F+   +K
Sbjct: 282827 DNYFTPYITVACAIGLTKQEIDTFV*EFEK 282738


>scaffold_6
          Length = 752274

 Score = 31.6 bits (70), Expect = 3.3
 Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 12/64 (18%)
 Frame = -2

Query: 131    VHSVASCFVVPMATG------------MSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKS 178
              VH+  +CFV+   TG            +S T CF+    K   A+ II P  D K   K 
Sbjct: 309884 VHASIACFVLTTTTGPY*TFANISLAVLSSTPCFIRTYGKTRSARLIINPSTDHKISCKK 309705

Query: 179    MVTA 182
               +T+
Sbjct: 309704 FITS 309693


>scaffold_158
          Length = 221018

 Score = 30.0 bits (66), Expect = 9.5
 Identities = 18/55 (32%), Positives = 27/55 (49%)
 Frame = +3

Query: 106   PKAAGSSLLNKITNSLVLNVIKLAGVHSVASCFVVPMATGMSLTLCFLTLRHKRP 160
             P    SS+ N+I N     ++KL   HS+   F++  +  + LT  FL   H RP
Sbjct: 90291 PPPTSSSMDNRICN-----ILKLMSTHSIDIWFLIEYS*TIELTY*FLCSFHSRP 90440


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,185,343
Number of Sequences: 697
Number of extensions: 426405
Number of successful extensions: 1243
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1217
Number of HSP's gapped (non-prelim): 92
length of query: 504
length of database: 23,960,378
effective HSP length: 110
effective length of query: 394
effective length of database: 23,883,708
effective search space: 9410180952
effective search space used: 9410180952
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 66 (30.0 bits)