TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|27369676|ref|NP_766078.1| O-phosphoseryl-tRNA(Sec) selenium
transferase [Mus musculus]
         (504 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_49                                                           460   e-129
scaffold_252                                                           31   2.6  

>scaffold_49 
          Length = 116333

 Score =  460 bits (1183), Expect = e-129
 Identities = 237/442 (53%), Positives = 316/442 (71%), Gaps = 23/442 (5%)
 Frame = +2

Query: 40    KCPEDGWDESTLELFLHELAVMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSG 99
             + PE G+ +  +EL L+E+++MDSNNF+ N GVGEREGR+ S+LV +RHY F HGIGRSG
Sbjct: 59207 RLPEIGYSDKVIELILNEISMMDSNNFIENIGVGEREGRIYSSLVEKRHYGFSHGIGRSG 59386

Query: 100   DISAVQPKAAGSSLLNKITNSLVLNVIKLAGVHSVAS--CFVVPMATGMSLTLCFLTLRH 157
             DI+  QPKAAGSSL+ K+ + LVL+ +++AG+ S A+  C ++PMATGM+L+L  +TL+ 
Sbjct: 59387 DITEQQPKAAGSSLIQKLVHYLVLDAMRIAGLESQAASTCLLLPMATGMTLSLAMMTLKQ 59566

Query: 158   K-RPKAKYIIWPRIDQKSCFKSMVTAGFEPVVIENVLEGDELRTDLKAVEAKIQELGPEH 216
             K +  A+Y+IWPRIDQKSC KS+VTAG  P+VIEN LEGD +RTDL A+++KI ELGP +
Sbjct: 59567 KSQSNARYVIWPRIDQKSCLKSIVTAGLIPIVIENKLEGDMIRTDLDAIKSKIIELGPSN 59746

Query: 217   ILCLHSTTACFAPRVPDRLEELAVICANYDIPHVVNNAYGLQSSKCMHLIQQGARVGRID 276
             ILC+ STT+CFAPR PDR+ E++ +C  ++I H++NNAYGLQ SK +H I Q  ++GR+D
Sbjct: 59747 ILCVFSTTSCFAPRAPDRIVEISQLCKEHNIGHIINNAYGLQCSKILHAISQSCKLGRVD 59926

Query: 277   AFVQSLDKNFMVPVGGAIIAGFNEPFIQDISKMYPGRASASPSLDVLITLLSLGCSGYRK 336
             A++QS DKNFMVPVGGAII+G N+ FI+DI+K YPGRA++SP LDV ITLLS+G SG+  
Sbjct: 59927 AYIQSTDKNFMVPVGGAIISGPNKVFIEDIAKNYPGRANSSPILDVFITLLSMGKSGWSG 60106

Query: 337   LLKERKEMFVYLSTQLKKLAEAHNERLLQT-PHNPISLAMTLKTI--------------- 380
             LLKERKE+ VY   QL KLA   NE+LL T   N IS A +LK                 
Sbjct: 60107 LLKERKELLVYFQDQLGKLANEFNEKLLSTINENKISFAFSLKNFSSNKNEQQLESELNN 60286

Query: 381   --DGHHDKAVTQLGSMLFTRQVSGARAVPLGNVQ--TVSGHTFRGFMSHADNYPCAYLNA 436
               + ++D + + LGS LF+R  SGAR + L   +   V G  F  + SH D YP +YL  
Sbjct: 60287 NNNNNNDYSPSMLGSQLFSRSCSGARVIDLSKNKKAIVGGLEFNYYGSHIDGYPTSYLTV 60466

Query: 437   AAAIGMKMQDVDLFIKRLDKCL 458
             A AIG+   ++D FI+RL K L
Sbjct: 60467 ACAIGVTKSEIDKFIQRLSKLL 60532


>scaffold_252 
          Length = 48726

 Score = 30.8 bits (68), Expect = 2.6
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -3

Query: 339   KERKEMFVYLSTQLKKLAEAHNERLLQTPHNPISLAMTLKTIDGH 383
             + RKE+  ++S +L +L  +H E LL +      L  TLK  DG+
Sbjct: 33310 QRRKELLNFISPKLVELCSSHTEDLLSSQWGTKVLNATLKFADGN 33176


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,954,302
Number of Sequences: 799
Number of extensions: 187357
Number of successful extensions: 637
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 610
Number of HSP's gapped (non-prelim): 57
length of query: 504
length of database: 10,989,169
effective HSP length: 105
effective length of query: 399
effective length of database: 10,905,274
effective search space: 4351204326
effective search space used: 4351204326
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)