TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|255071835|ref|XP_002499592.1| O-phosphoseryl-tRNA selenium
transferase [Micromonas sp. RCC299]
         (498 letters)

Database: genome.fa 
           32,746 sequences; 89,612,356 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAHK01001140.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   299   9e-81
gb|AAHK01001263.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   298   2e-80
gb|AAHK01002877.1| Trypanosoma cruzi strain CL Brener tcruzi_104...    32   2.1  
gb|AAHK01000598.1| Trypanosoma cruzi strain CL Brener tcruzi_104...    32   3.5  

>gb|AAHK01001140.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053506389, whole genome
             shotgun sequence
          Length = 16727

 Score =  299 bits (765), Expect = 9e-81
 Identities = 192/515 (37%), Positives = 267/515 (51%), Gaps = 67/515 (13%)
 Frame = +2

Query: 6     AYALAEGLVSKAYINQGSQATARRSKLVTSLLSERRLPKDGWDDHSIESFLSEAAMMDSN 65
             A +LAEG VS  Y+  G +A     +LV S+L++RR P+ G  D +IE  L + A+MD+N
Sbjct: 10148 ALSLAEGFVSANYVQAGGEALRANERLVRSVLAQRRCPEKGMSDVAIEWLLHQLALMDTN 10327

Query: 66    NFLDNVGVGEREARVYSPLVARRHWNLAHGIGRSGDVAAEQPKAAGSSLLNKLTNLLVAD 125
             NF  +VG GERE RV +PLVARR++ LAHGIGRSGD+ ++QPKAAGSSL++KLTN ++ D
Sbjct: 10328 NFPAHVGAGEREGRVAAPLVARRNYGLAHGIGRSGDILSDQPKAAGSSLIHKLTNCMMLD 10507

Query: 126   AMRIAGLEDMGPALVLPLATGMTLTLVLLAVKSKRPKTATK----------------VVW 169
              +R+AG   +  A+V P+ATGMTL+LVL A+   R     K                V+W
Sbjct: 10508 LLRLAGAPSIESAVVFPMATGMTLSLVLRALALLRRDEKRKEMPDLLKGAVEDEPRYVIW 10687

Query: 170   CRLDQKTCVKAVLSAGLELVVVSPKL------------VGDQLCTDL--------DXXXX 209
              R+DQKT +K + +AG    +V  +             +G + C           D    
Sbjct: 10688 ARIDQKTALKCIEAAGFVPEIVELRHAPLQRKGTCGLGLGSETCLHPFFLQVHLDDIVAA 10867

Query: 210   XXXXXXXXXXCCVVTSTSCFAPRAGDACSDVAKMCAELDVGHVVNNAYGVQATALCKEVT 269
                        CV+++TSCFAPR  D    +AK C  +++ +VVNNAYGVQ+  + + + 
Sbjct: 10868 IQRVGGPKHVLCVLSTTSCFAPRIPDDVLAIAKHCKFMNIPYVVNNAYGVQSRVIMRRLD 11047

Query: 270   KAWRRGRVDAVVQSTDKNFMVPVGGAVVCSGKGNTEIVDAVRKSYPGRASISPVLDLLIT 329
              A R GR+DA+VQS DKNF+VPVGGAV+    G   +V+     Y GRASISP++DL IT
Sbjct: 11048 AAMRLGRIDAMVQSGDKNFLVPVGGAVL---SGKKHVVERAAALYAGRASISPIVDLFIT 11218

Query: 330   LLSMGSHGWQRCLEDRGDVYGYMRTKLGECVGEFGERLLD-------------------- 369
              LSMG  G+QR  E R +V   ++  L       GE LL+                    
Sbjct: 11219 ALSMGRGGFQRLWEVRYEVLNMVQQGLRRFAMARGEVLLEEEDVNDIAASASAAGSTGAA 11398

Query: 370   ---------TPGNPISIGVSLETHAKLSEDGKDVSFFGSMLFSRCVSGTRVVAPGK--RQ 418
                      T  N IS  V++ T         +    G+ LF   V+G RV+ P    +Q
Sbjct: 11399 ATASCTVRGTHRNDISFAVTMHTVG----GAANAKAIGARLFRGAVTGPRVIVPDSTIKQ 11566

Query: 419   XXXXXXXXXXXASHDAYPVPYFTAAAALGTTREDV 453
                         + +  P P    A  +GTT E+V
Sbjct: 11567 LCGLSFRNYGMHTDEVLPCPLLVMACGIGTTAEEV 11671


>gb|AAHK01001263.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053507483, whole genome
             shotgun sequence
          Length = 14792

 Score =  298 bits (763), Expect = 2e-80
 Identities = 191/515 (37%), Positives = 267/515 (51%), Gaps = 67/515 (13%)
 Frame = -2

Query: 6     AYALAEGLVSKAYINQGSQATARRSKLVTSLLSERRLPKDGWDDHSIESFLSEAAMMDSN 65
             A +LAEG VS  Y+  G +A     +LV S+L++RR P+ G  D +IE  L + A+MD+N
Sbjct: 14575 ALSLAEGFVSAHYVQAGGEALRANERLVRSVLAQRRCPEKGMSDVAIEWLLHQLALMDTN 14396

Query: 66    NFLDNVGVGEREARVYSPLVARRHWNLAHGIGRSGDVAAEQPKAAGSSLLNKLTNLLVAD 125
             NF  +VG GERE RV +PLVARR++ LAHGIGRSGD+ ++QPKAAGSSL++KLTN ++ D
Sbjct: 14395 NFPAHVGAGEREGRVAAPLVARRNYGLAHGIGRSGDILSDQPKAAGSSLIHKLTNCMMLD 14216

Query: 126   AMRIAGLEDMGPALVLPLATGMTLTLVLLAVKSKRPKTATK----------------VVW 169
              +R+AG   +  A+V P+ATGMTL+LVL A+   R     K                V+W
Sbjct: 14215 LLRLAGAPSIESAVVFPMATGMTLSLVLRALALLRRDERRKEMPDLLKGAVEDEPRYVIW 14036

Query: 170   CRLDQKTCVKAVLSAGLELVVVSPKL------------VGDQLCTDL--------DXXXX 209
              R+DQKT +K + +AG    +V  +             +G + C           D    
Sbjct: 14035 ARIDQKTALKCIEAAGFVPELVELRHAPLQRKGTCGLGLGSETCLHPFFLQVHPDDIVAA 13856

Query: 210   XXXXXXXXXXCCVVTSTSCFAPRAGDACSDVAKMCAELDVGHVVNNAYGVQATALCKEVT 269
                        CV+++TSCFAPR  D    +AK C  +++ +VVNNAYGVQ+  + + + 
Sbjct: 13855 IQRVGGPKHVLCVLSTTSCFAPRIPDDVLAIAKHCKAINIPYVVNNAYGVQSRVIMRRLD 13676

Query: 270   KAWRRGRVDAVVQSTDKNFMVPVGGAVVCSGKGNTEIVDAVRKSYPGRASISPVLDLLIT 329
              A R GR+DA+VQS DKNF+VPVGGAV+    G   +V+     Y GRASISP++DL IT
Sbjct: 13675 AAMRLGRIDAMVQSGDKNFLVPVGGAVL---SGKKHVVERAAALYAGRASISPIVDLFIT 13505

Query: 330   LLSMGSHGWQRCLEDRGDVYGYMRTKLGECVGEFGERLLD-------------------- 369
              LSMG  G+QR  + R +V   ++  L       GE LL+                    
Sbjct: 13504 ALSMGRSGFQRLWDVRYEVLNMVQQGLRRFAMARGEVLLEEEDVNDIAASASAAGFTGAV 13325

Query: 370   ---------TPGNPISIGVSLETHAKLSEDGKDVSFFGSMLFSRCVSGTRVVAPGK--RQ 418
                      T  N IS  V++ T         +    G+ LF   V+G RV+ P    +Q
Sbjct: 13324 ATASCTVRGTNRNDISFAVTMHTVG----GAANAKAIGARLFRGAVTGPRVIVPDSTIKQ 13157

Query: 419   XXXXXXXXXXXASHDAYPVPYFTAAAALGTTREDV 453
                         + +  P P    A  +GTT E+V
Sbjct: 13156 LCGLSFRNYGMHADEVLPCPLLVMACGIGTTAEEV 13052


>gb|AAHK01002877.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053504891, whole genome
            shotgun sequence
          Length = 4412

 Score = 32.3 bits (72), Expect = 2.1
 Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 2/58 (3%)
 Frame = +3

Query: 46   GWD-DHSIESFLSEAAMMDSNN-FLDNVGVGEREARVYSPLVARRHWNLAHGIGRSGD 101
            GW+ +H I++F+    M+D NN +LD++   +       P VAR   +  +G G +GD
Sbjct: 1167 GWNGEHDIDAFM---VMLDENNCYLDHLHPKQTRLHSIYPHVARHSGDAINGFGTTGD 1331


>gb|AAHK01000598.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053509011, whole genome
             shotgun sequence
          Length = 29233

 Score = 31.6 bits (70), Expect = 3.5
 Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 2/58 (3%)
 Frame = +1

Query: 46    GWD-DHSIESFLSEAAMMDSNN-FLDNVGVGEREARVYSPLVARRHWNLAHGIGRSGD 101
             GW+ +H +++F+    M+D NN +LD++   +       P VAR   +  +G G +GD
Sbjct: 14398 GWNGEHDLDAFM---VMLDENNCYLDHLHPKQTRLHSIYPHVARHSGDAINGFGTTGD 14562


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:13 PM
  Number of letters in database: 89,612,356
  Number of sequences in database:  32,746
  
Lambda     K      H
   0.319    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,104,978
Number of Sequences: 32746
Number of extensions: 824981
Number of successful extensions: 3509
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 2458
Number of HSP's successfully gapped in prelim test: 115
Number of HSP's that attempted gapping in prelim test: 672
Number of HSP's gapped (non-prelim): 3053
length of query: 498
length of database: 29,870,785
effective HSP length: 111
effective length of query: 387
effective length of database: 26,235,979
effective search space: 10153323873
effective search space used: 10153323873
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)