TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|255071835|ref|XP_002499592.1| O-phosphoseryl-tRNA selenium
transferase [Micromonas sp. RCC299]
         (498 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000100.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    30   2.1  
gb|AAFB02000123.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    29   4.6  

>gb|AAFB02000100.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750507712, whole genome
             shotgun sequence
          Length = 44003

 Score = 30.4 bits (67), Expect = 2.1
 Identities = 21/64 (32%), Positives = 33/64 (51%)
 Frame = +3

Query: 43    PKDGWDDHSIESFLSEAAMMDSNNFLDNVGVGEREARVYSPLVARRHWNLAHGIGRSGDV 102
             P+ GWD  S+E  LS   ++ SNN L  VG+   +  +   ++A      A GIG S D+
Sbjct: 19365 PQRGWDVPSVECILSTQEIIISNNLL--VGLPAPDFSMPYNVLALSLAIFALGIGNSIDI 19538

Query: 103   AAEQ 106
               ++
Sbjct: 19539 VIQK 19550


>gb|AAFB02000123.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750505577, whole genome
            shotgun sequence
          Length = 40236

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 28/121 (23%), Positives = 45/121 (37%), Gaps = 9/121 (7%)
 Frame = -2

Query: 232  RAGDACSDVAKMCAELDVGHVVNNAYGVQATALCKEVTKAWRRGRVDAVVQSTDKNFMVP 291
            +A   CS    +C E D      + + +  T  C E+         D   +  DK + + 
Sbjct: 7076 KACTKCSSFCSLCTESDNCFQCTDKHYLNGTYNCLEIDHCDSSSIKDDHCEKCDKKYRLN 6897

Query: 292  VGGAVVCSGKGNTE-IVDA--VRKSYPGRASISPVLDLLITLLSMGS------HGWQRCL 342
             GG V C+ +   E  +D     K +       P     ITL S+        +G QRC+
Sbjct: 6896 GGGCVACTIENCDECFIDEKNQEKCFKCLTGFIPYNGQCITLKSINCEVGSEIYGCQRCV 6717

Query: 343  E 343
            +
Sbjct: 6716 D 6714


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.319    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,882,670
Number of Sequences: 2172
Number of extensions: 65482
Number of successful extensions: 172
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 142
Number of HSP's gapped (non-prelim): 40
length of query: 498
length of database: 6,923,698
effective HSP length: 101
effective length of query: 397
effective length of database: 6,704,326
effective search space: 2661617422
effective search space used: 2661617422
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)