TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium
transferase [Homo sapiens]
         (501 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_7                                                            354   1e-98

>scaffold_7
          Length = 741107

 Score =  354 bits (908), Expect(2) = 1e-98
 Identities = 186/422 (44%), Positives = 277/422 (65%), Gaps = 5/422 (1%)
 Frame = -3

Query: 12     LVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCG 71
              ++   YV+    +  + E L +LLL   + PE G+D+ T+E  L+  A MD+NNF    G
Sbjct: 284112 IIDDKYVKIASNSLNAKEKLFKLLLS*RQLPEEGFDDLTIEYLLNIFATMDTNNFSNKVG 283933

Query: 72     VGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGV 131
              VGERE R+ SALV +R+Y   HGIGRSGD+ A QPKAAGSSL+ K+T  L  D +KL+  
Sbjct: 283932 VGERESRIFSALV*KRNYYMGHGIGRSGDLIASQPKAAGSSLIAKLTKHLAKDALKLSN- 283756

Query: 132    HTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIEN 191
              + +    +VP+ATGM+++LC L L+  +P AKY++WPRID K+C K + TA   P+VI++
Sbjct: 283755 YQINELLLVPVATGMAISLCLLALKSLKP*AKYVLWPRID*KTCLKCI*TANLIPIVIDS 283576

Query: 192    VLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIV 251
              V++   + T+L  +  KV E+GPD I+C+ STTSCFAPR+PD + E+A IC  + I H+V
Sbjct: 283575 VIQDHVVTTNLDMLI*KVNEIGPDNIVCVLSTTSCFAPRIPDNIGEIAKICK*FSIYHVV 283396

Query: 252    NNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYP 311
              NNAYG+  +K  + I  G   G ++  V S DKNFMVPVGGA+I G +   I+ IS++YP
Sbjct: 283395 NNAYGL*CNKIANSINLGLA*GTVNLIVSSTDKNFMVPVGGALIYGNDKKLIK*ISELYP 283216

Query: 312    GRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPI 371
              GRASA+P LD+ IT LS+G +GY +L KER E ++Y   ++  ++   +E++L+TP N I
Sbjct: 283215 GRASAAPILDLFITFLSMGKSGYLQLQKERVENYAYFKEKL*IMT*KISEQILNTPKNSI 283036

Query: 372    SLAMTLKTL-----DEHRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHT 426
              S+ ++L  L     +      +T+LG++L++++V G+RV+     + V G  F+ + SH 
Sbjct: 283035 SIGISL*NLKID*NN*EEKN*ITKLGAILYSKKVMGSRVITNKKNKVVCGIEFKNYGSHC 282856

Query: 427    NN 428
               N
Sbjct: 282855 GN 282850



 Score = 26.9 bits (58), Expect(2) = 1e-98
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -1

Query: 427    NNYPCAYLNAASAIGMKMQDVDLFI 451
              +NY   Y+  A AIG+  Q++D F+
Sbjct: 282827 DNYFTPYITVACAIGLTKQEIDTFV 282753


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.320    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,741,738
Number of Sequences: 697
Number of extensions: 466027
Number of successful extensions: 1583
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1535
Number of HSP's gapped (non-prelim): 131
length of query: 501
length of database: 23,960,378
effective HSP length: 110
effective length of query: 391
effective length of database: 23,883,708
effective search space: 9338529828
effective search space used: 9338529828
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)