TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium
transferase [Homo sapiens]
         (501 letters)

Database: genome.fa 
           1696 sequences; 31,706,682 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

mex400p23.q1k                                                         288   7e-78
mex120o20.q1k                                                          31   2.4  
mex309d04.q1k                                                          29   7.1  
mex454c03.q1k                                                          29   9.3  

>mex400p23.q1k 
          Length = 34044

 Score =  288 bits (737), Expect = 7e-78
 Identities = 202/587 (34%), Positives = 281/587 (47%), Gaps = 133/587 (22%)
 Frame = +2

Query: 1    MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAI 60
            M+  +    E  VS  Y+  G E+ +S   ++R +L +  CP++G  ++ +EL L +L++
Sbjct: 5945 MDDRALKLAEAFVSARYIDAGRESLQSTARILRSILAQRCCPDDGLTDAAIELILRQLSL 6124

Query: 61   MDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNS 120
            MD+NNF  + G GEREGRV S+LV  RH+   HGIGRSGD+ + QPKAAGSSLL KITN 
Sbjct: 6125 MDTNNFAHHVGGGEREGRVISSLVRMRHFGLTHGIGRSGDVLSEQPKAAGSSLLYKITNV 6304

Query: 121  LVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTL------------------------- 155
            L+LD+I+ AG  + A   VVPMATGM+L L    +                         
Sbjct: 6305 LMLDLIRQAGAPSTAAAVVVPMATGMTLALVLRCVAKAHMKELMEEAEAVQLQRTVTTDS 6484

Query: 156  --------------------RHKRPKA------KYIIWPRIDQKSCFKSMITAGFEPVVI 189
                                RH R         +Y+IWPRIDQK+  K +  AG  PV +
Sbjct: 6485 RSATSAAQVQEPPMNEADRARHDRTSLPVPATPRYVIWPRIDQKTALKCIDAAGLVPVPV 6664

Query: 190  E-------------------NVLEGDE----------------LRTDLKAVEAKVQELG- 213
            +                   N L+ D+                L   +  V A V  +G 
Sbjct: 6665 QLRPAVPLARSAAPCVSTNRNNLDRDQDSIASPSTRTSSSSLFLECHVDDVAAAVSAVGG 6844

Query: 214  PDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVG 273
            P  ++C+ STTSCFAPR+PD    LA  C    IP++VNNAYGVQS + M  +    R+G
Sbjct: 6845 PSQVVCVLSTTSCFAPRLPDNTVALAQYCKKAGIPYVVNNAYGVQSRRIMTRLDAAQRLG 7024

Query: 274  RIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNG 333
            R+D  VQS DKNF+VPVGG+II   +    + ++ +Y GRAS SP +D+ IT LSLG  G
Sbjct: 7025 RVDFVVQSGDKNFLVPVGGSIICSSDKERCKAVAALYAGRASMSPIVDLFITALSLGRRG 7204

Query: 334  YKKLLKERKEMFSYLSNQIKKLSEAYNERLL-----------------------HTPHNP 370
             + L ++R +  + L  Q++  +    E LL                         P N 
Sbjct: 7205 MQALWRDRYKCRARLIRQLRVFARERREVLLVDDSDDDKDNEDTVGGSQNTSNAAVPRND 7384

Query: 371  ISLAMTLKTL---------------DEHRDK-----AVTQLGSMLFTRQVSGARVV-PLG 409
            IS+A+T++                  E  +K     A    G+ LF   V+G RV+ P  
Sbjct: 7385 ISVAVTMRAYGLPAAEASSSGAQLGSEQAEKVTNWAAARAFGAQLFRSAVTGPRVITPAP 7564

Query: 410  SM-QTVSGYTFRGFMSHTNNY-PCAYLNAASAIGMKMQDVDLFIKRL 454
            S   T++G  FR +  H +   PC  L  A  IGM   +VD  I RL
Sbjct: 7565 STPTTIAGCKFRNYGMHQDREPPCPLLVIACGIGMSEAEVDALIARL 7705


>mex120o20.q1k 
          Length = 41957

 Score = 30.8 bits (68), Expect = 2.4
 Identities = 16/53 (30%), Positives = 28/53 (52%)
 Frame = -2

Query: 185   EPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDRLEE 237
             E  +++N    +E R DLKA  A+V+ + PD ++ +   +       P RLE+
Sbjct: 14035 ERALVQNAAACEEARDDLKAQ*ARVEHISPDNVVRLVHASRRGGVTAPRRLED 13877


>mex309d04.q1k 
          Length = 34001

 Score = 29.3 bits (64), Expect = 7.1
 Identities = 27/87 (31%), Positives = 37/87 (42%), Gaps = 5/87 (5%)
 Frame = +1

Query: 82    ALVARRHYRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVP 141
             A +  RH+   HG G     S++  +  GSS    +T+SL  +       H+VA+  V  
Sbjct: 12694 AAMGARHHNHQHGAG-----SSLSERREGSSFCTAVTSSLKSETHW*RSAHSVADTAVAR 12858

Query: 142   MATGM--SLTLCF---LTLRHKRPKAK 163
             M      SL   F   L  R  RPK K
Sbjct: 12859 MRWSKI*SLRASFSGALPQRSPRPKLK 12939


>mex454c03.q1k 
          Length = 27867

 Score = 28.9 bits (63), Expect = 9.3
 Identities = 22/91 (24%), Positives = 38/91 (41%), Gaps = 10/91 (10%)
 Frame = -1

Query: 303   IQEISKMYPGRASASPSLDVLITLLSL----------GSNGYKKLLKERKEMFSYLSNQI 352
             ++E+ +MY  +A+      VL   LS            +N +   L   KEM +    + 
Sbjct: 23739 LRELRRMYTSQAALPTEFSVLKAKLSSTTPLIWAKCRSNNDFVTFLPALKEMIALARREA 23560

Query: 353   KKLSEAYNERLLHTPHNPISLAMTLKTLDEH 383
             +  S A  + L     N     MTL+TL+++
Sbjct: 23559 QYRSTATGKPLYEALFNQYESGMTLETLEKN 23467


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 31,706,682
  Number of sequences in database:  1696
  
Lambda     K      H
   0.320    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,226,310
Number of Sequences: 1696
Number of extensions: 301385
Number of successful extensions: 1813
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 77
Number of HSP's that attempted gapping in prelim test: 1522
Number of HSP's gapped (non-prelim): 583
length of query: 501
length of database: 10,568,894
effective HSP length: 104
effective length of query: 397
effective length of database: 10,392,510
effective search space: 4125826470
effective search space used: 4125826470
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)