TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium
transferase [Homo sapiens]
         (501 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000125.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    30   2.1  
gb|AAFB02000130.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    29   4.6  
gb|AAFB02000083.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   7.9  

>gb|AAFB02000125.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514861, whole genome
             shotgun sequence
          Length = 39577

 Score = 30.4 bits (67), Expect = 2.1
 Identities = 26/100 (26%), Positives = 46/100 (46%)
 Frame = +3

Query: 275   IDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGY 334
             I  F++S  K+F +         F DSFI+ +      R     S  +L ++ +      
Sbjct: 17733 ISTFIESTKKDFSLLCHCP--ETFFDSFIKFLPHNMKHRLLQFSSTSLLPSIKT------ 17888

Query: 335   KKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLA 374
              K ++ER + F YL+ Q+K++   +   +   P NPI L+
Sbjct: 17889 -KKIQERYK-FDYLAQQVKEIQHDWRNEVTQKPLNPIQLS 18002


>gb|AAFB02000130.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750506487, whole genome
             shotgun sequence
          Length = 37909

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 26/106 (24%), Positives = 49/106 (46%), Gaps = 3/106 (2%)
 Frame = +3

Query: 13    VSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGV 72
             V P    +  +  +S E++++ L +K K PEN   +   ELFLH   + D +++      
Sbjct: 15642 VGPITAFELIQQYKSIENILQHLSDKYKVPENWKYKEARELFLHP-DVADFSDYKLEWNK 15818

Query: 73    GEREGRVASALVARRHY---RFIHGIGRSGDISAVQPKAAGSSLLN 115
              + EG +   LV  +H+   R   GI +  ++ + + +    S  N
Sbjct: 15819 IDEEG-IKKYLVTEKHFNEERVSKGIEKLKNVKSKKAQGRLDSFFN 15953


>gb|AAFB02000083.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750514659, whole genome
             shotgun sequence
          Length = 47565

 Score = 28.5 bits (62), Expect = 7.9
 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 5/57 (8%)
 Frame = -2

Query: 297   GFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGY-----KKLLKERKEMFSYL 348
             G ND+F+ EI    P + ++   L  +I  +SL +NG      K   KE+K  F+ L
Sbjct: 33425 GKNDTFMDEIKAKLPTKLTSQHHLLFVIQDVSLDNNGIDDKTDKNEQKEKKTFFASL 33255


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.320    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,223,428
Number of Sequences: 2172
Number of extensions: 142015
Number of successful extensions: 500
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 90
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 388
Number of HSP's gapped (non-prelim): 184
length of query: 501
length of database: 6,923,698
effective HSP length: 101
effective length of query: 400
effective length of database: 6,704,326
effective search space: 2681730400
effective search space used: 2681730400
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)