TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium
transferase [Homo sapiens]
         (501 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_49                                                           456   e-128

>scaffold_49 
          Length = 116333

 Score =  456 bits (1172), Expect = e-128
 Identities = 231/443 (52%), Positives = 315/443 (71%), Gaps = 23/443 (5%)
 Frame = +2

Query: 40    KCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSG 99
             + PE G+ +  +EL L+E+++MDSNNF+ N GVGEREGR+ S+LV +RHY F HGIGRSG
Sbjct: 59207 RLPEIGYSDKVIELILNEISMMDSNNFIENIGVGEREGRIYSSLVEKRHYGFSHGIGRSG 59386

Query: 100   DISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVA--NCFVVPMATGMSLTLCFLTLRH 157
             DI+  QPKAAGSSL+ K+ + LVLD +++AG+ + A   C ++PMATGM+L+L  +TL+ 
Sbjct: 59387 DITEQQPKAAGSSLIQKLVHYLVLDAMRIAGLESQAASTCLLLPMATGMTLSLAMMTLKQ 59566

Query: 158   K-RPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDC 216
             K +  A+Y+IWPRIDQKSC KS++TAG  P+VIEN LEGD +RTDL A+++K+ ELGP  
Sbjct: 59567 KSQSNARYVIWPRIDQKSCLKSIVTAGLIPIVIENKLEGDMIRTDLDAIKSKIIELGPSN 59746

Query: 217   ILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRID 276
             ILC+ STTSCFAPR PDR+ E++ +C  ++I HI+NNAYG+Q SK +H I Q  ++GR+D
Sbjct: 59747 ILCVFSTTSCFAPRAPDRIVEISQLCKEHNIGHIINNAYGLQCSKILHAISQSCKLGRVD 59926

Query: 277   AFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKK 336
             A++QS DKNFMVPVGGAII+G N  FI++I+K YPGRA++SP LDV ITLLS+G +G+  
Sbjct: 59927 AYIQSTDKNFMVPVGGAIISGPNKVFIEDIAKNYPGRANSSPILDVFITLLSMGKSGWSG 60106

Query: 337   LLKERKEMFSYLSNQIKKLSEAYNERLLHT-PHNPISLAMTLKTLDEHR----------- 384
             LLKERKE+  Y  +Q+ KL+  +NE+LL T   N IS A +LK    ++           
Sbjct: 60107 LLKERKELLVYFQDQLGKLANEFNEKLLSTINENKISFAFSLKNFSSNKNEQQLESELNN 60286

Query: 385   ------DKAVTQLGSMLFTRQVSGARVVPLGSMQ--TVSGYTFRGFMSHTNNYPCAYLNA 436
                   D + + LGS LF+R  SGARV+ L   +   V G  F  + SH + YP +YL  
Sbjct: 60287 NNNNNNDYSPSMLGSQLFSRSCSGARVIDLSKNKKAIVGGLEFNYYGSHIDGYPTSYLTV 60466

Query: 437   ASAIGMKMQDVDLFIKRLDRCLK 459
             A AIG+   ++D FI+RL + LK
Sbjct: 60467 ACAIGVTKSEIDKFIQRLSKLLK 60535


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.320    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,587,620
Number of Sequences: 799
Number of extensions: 206618
Number of successful extensions: 852
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 803
Number of HSP's gapped (non-prelim): 91
length of query: 501
length of database: 10,989,169
effective HSP length: 105
effective length of query: 396
effective length of database: 10,905,274
effective search space: 4318488504
effective search space used: 4318488504
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)