TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: genome.fa 
           32,746 sequences; 89,612,356 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAHK01001140.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   292   8e-79
gb|AAHK01001263.1| Trypanosoma cruzi strain CL Brener tcruzi_104...   291   1e-78

>gb|AAHK01001140.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053506389, whole genome
             shotgun sequence
          Length = 16727

 Score =  292 bits (748), Expect = 8e-79
 Identities = 187/523 (35%), Positives = 273/523 (52%), Gaps = 51/523 (9%)
 Frame = +2

Query: 1     MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
             MN + L   +G +  +Y+    +      +L+ ++L  ++ P  G +D  IE +L++++L
Sbjct: 10136 MNERALSLAEGFVSANYVQAGGEALRANERLVRSVLAQRRCPEKGMSDVAIEWLLHQLAL 10315

Query: 61    MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
             MD+NNF  ++G GEREGR+ + LV +R+YG AHGIGRSGDI   QPKAAGSSLI KLT+ 
Sbjct: 10316 MDTNNFPAHVGAGEREGRVAAPLVARRNYGLAHGIGRSGDILSDQPKAAGSSLIHKLTNC 10495

Query: 121   LVLDAMKLAG---LEQS-----------SLSNCXXXXXXXXXXXXXXXXXXKSINANNKR 166
             ++LD ++LAG   +E +           SL                     K    +  R
Sbjct: 10496 MMLDLLRLAGAPSIESAVVFPMATGMTLSLVLRALALLRRDEKRKEMPDLLKGAVEDEPR 10675

Query: 167   YVLWPRIDQKSCLKSIITAGLIPIVI-----PNQLDG-----------------DMIRTD 204
             YV+W RIDQK+ LK I  AG +P ++     P Q  G                  +   D
Sbjct: 10676 YVIWARIDQKTALKCIEAAGFVPEIVELRHAPLQRKGTCGLGLGSETCLHPFFLQVHLDD 10855

Query: 205   LVAIEDKIKELGVDNILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINNAYGLQCSK 264
             +VA   ++   G  ++LCV STTSCFAPR+PD ++ I++ CK  NI +++NNAYG+Q   
Sbjct: 10856 IVAAIQRVG--GPKHVLCVLSTTSCFAPRIPDDVLAIAKHCKFMNIPYVVNNAYGVQSRV 11029

Query: 265   ILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYPGRANSSPILD 324
             I+  +  A +LGR+DA +QS DKNF+VPVGGA++SG     +++ +  Y GRA+ SPI+D
Sbjct: 11030 IMRRLDAAMRLGRIDAMVQSGDKNFLVPVGGAVLSG-KKHVVERAAALYAGRASISPIVD 11206

Query: 325   VFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFALENNEKLLNTINENKI--------- 375
             +FIT LSMG+ G+  L + R E+L    + L +FA+   E LL   + N I         
Sbjct: 11207 LFITALSMGRGGFQRLWEVRYEVLNMVQQGLRRFAMARGEVLLEEEDVNDIAASASAAGS 11386

Query: 376   -----SFAXXXXXXXXXXXXXXXXXXXXXXXXXXXMIGSKLFSRSCSGSRVIDLKSNKKL 430
                  + +                            IG++LF  + +G RVI   S  K 
Sbjct: 11387 TGAAATASCTVRGTHRNDISFAVTMHTVGGAANAKAIGARLFRGAVTGPRVIVPDSTIKQ 11566

Query: 431   LIGGLEFNNYGSHIDN-YSTSYLTVACAIGITKLEIDTFIQRL 472
             L  GL F NYG H D       L +AC IG T  E+   ++RL
Sbjct: 11567 LC-GLSFRNYGMHTDEVLPCPLLVMACGIGTTAEEVRGVMERL 11692


>gb|AAHK01001263.1| Trypanosoma cruzi strain CL Brener tcruzi_1047053507483, whole genome
             shotgun sequence
          Length = 14792

 Score =  291 bits (746), Expect = 1e-78
 Identities = 187/523 (35%), Positives = 271/523 (51%), Gaps = 51/523 (9%)
 Frame = -2

Query: 1     MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
             MN + L   +G +   Y+    +      +L+ ++L  ++ P  G +D  IE +L++++L
Sbjct: 14587 MNERALSLAEGFVSAHYVQAGGEALRANERLVRSVLAQRRCPEKGMSDVAIEWLLHQLAL 14408

Query: 61    MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
             MD+NNF  ++G GEREGR+ + LV +R+YG AHGIGRSGDI   QPKAAGSSLI KLT+ 
Sbjct: 14407 MDTNNFPAHVGAGEREGRVAAPLVARRNYGLAHGIGRSGDILSDQPKAAGSSLIHKLTNC 14228

Query: 121   LVLDAMKLAG---LEQS-----------SLSNCXXXXXXXXXXXXXXXXXXKSINANNKR 166
             ++LD ++LAG   +E +           SL                     K    +  R
Sbjct: 14227 MMLDLLRLAGAPSIESAVVFPMATGMTLSLVLRALALLRRDERRKEMPDLLKGAVEDEPR 14048

Query: 167   YVLWPRIDQKSCLKSIITAGLIPIVI-----PNQLDG-----------------DMIRTD 204
             YV+W RIDQK+ LK I  AG +P ++     P Q  G                  +   D
Sbjct: 14047 YVIWARIDQKTALKCIEAAGFVPELVELRHAPLQRKGTCGLGLGSETCLHPFFLQVHPDD 13868

Query: 205   LVAIEDKIKELGVDNILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINNAYGLQCSK 264
             +VA   ++   G  ++LCV STTSCFAPR+PD ++ I++ CK  NI +++NNAYG+Q   
Sbjct: 13867 IVAAIQRVG--GPKHVLCVLSTTSCFAPRIPDDVLAIAKHCKAINIPYVVNNAYGVQSRV 13694

Query: 265   ILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYPGRANSSPILD 324
             I+  +  A +LGR+DA +QS DKNF+VPVGGA++SG     +++ +  Y GRA+ SPI+D
Sbjct: 13693 IMRRLDAAMRLGRIDAMVQSGDKNFLVPVGGAVLSG-KKHVVERAAALYAGRASISPIVD 13517

Query: 325   VFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFALENNEKLLNTINENKI--------- 375
             +FIT LSMG+ G+  L   R E+L    + L +FA+   E LL   + N I         
Sbjct: 13516 LFITALSMGRSGFQRLWDVRYEVLNMVQQGLRRFAMARGEVLLEEEDVNDIAASASAAGF 13337

Query: 376   -----SFAXXXXXXXXXXXXXXXXXXXXXXXXXXXMIGSKLFSRSCSGSRVIDLKSNKKL 430
                  + +                            IG++LF  + +G RVI   S  K 
Sbjct: 13336 TGAVATASCTVRGTNRNDISFAVTMHTVGGAANAKAIGARLFRGAVTGPRVIVPDSTIKQ 13157

Query: 431   LIGGLEFNNYGSHIDN-YSTSYLTVACAIGITKLEIDTFIQRL 472
             L  GL F NYG H D       L +AC IG T  E+   ++RL
Sbjct: 13156 LC-GLSFRNYGMHADEVLPCPLLVMACGIGTTAEEVRGVMERL 13031


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:13 PM
  Number of letters in database: 89,612,356
  Number of sequences in database:  32,746
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,474,433
Number of Sequences: 32746
Number of extensions: 516320
Number of successful extensions: 1532
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1121
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 323
Number of HSP's gapped (non-prelim): 1280
length of query: 479
length of database: 29,870,785
effective HSP length: 110
effective length of query: 369
effective length of database: 26,268,725
effective search space: 9693159525
effective search space used: 9693159525
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)