TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_7                                                            340   2e-93
scaffold_45                                                            30   6.8  
scaffold_13                                                            30   8.9  

>scaffold_7
          Length = 741107

 Score =  340 bits (873), Expect = 2e-93
 Identities = 204/446 (45%), Positives = 269/446 (60%)
 Frame = -3

Query: 1      MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
              MN  NL+ C  +I   Y+  A    +   KL + LL  ++LP  G++D  IE +LN  + 
Sbjct: 284145 MN**NLKLCSEIIDDKYVKIASNSLNAKEKLFKLLLS*RQLPEEGFDDLTIEYLLNIFAT 283966

Query: 61     MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
              MD+NNF   +GVGERE RI+S LV KR+Y   HGIGRSGD+   QPKAAGSSLI KLT  
Sbjct: 283965 MDTNNFSNKVGVGERESRIFSALV*KRNYYMGHGIGRSGDLIASQPKAAGSSLIAKLTKH 283786

Query: 121    LVLDAMKLAGLEQSSLSNCXXXXXXXXXXXXXXXXXXKSINANNKRYVLWPRIDQKSCLK 180
              L  DA+KL+  + + L                     KS+    K YVLWPRID K+CLK
Sbjct: 283785 LAKDALKLSNYQINEL---LLVPVATGMAISLCLLALKSLKP*AK-YVLWPRID*KTCLK 283618

Query: 181    SIITAGLIPIVIPNQLDGDMIRTDLVAIEDKIKELGVDNILCVFSTTSCFAPRVPDKIIE 240
               I TA LIPIVI + +   ++ T+L  +  K+ E+G DNI+CV STTSCFAPR+PD I E
Sbjct: 283617 CI*TANLIPIVIDSVIQDHVVTTNLDMLI*KVNEIGPDNIVCVLSTTSCFAPRIPDNIGE 283438

Query: 241    ISEICKRYNIGHIINNAYGLQCSKILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISG 300
              I++ICK ++I H++NNAYGL C+KI ++I+     G V+  + STDKNFMVPVGGA+I G
Sbjct: 283437 IAKICK*FSIYHVVNNAYGL*CNKIANSINLGLA*GTVNLIVSSTDKNFMVPVGGALIYG 283258

Query: 301    PNSEFIDQISRNYPGRANSSPILDVFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFAL 360
               + + I  IS  YPGRA+++PILD+FIT LSMGK G+L L KER E   YF E+L     
Sbjct: 283257 NDKKLIK*ISELYPGRASAAPILDLFITFLSMGKSGYLQLQKERVENYAYFKEKL*IMT* 283078

Query: 361    ENNEKLLNTINENKISFAXXXXXXXXXXXXXXXXXXXXXXXXXXXMIGSKLFSRSCSGSR 420
              + +E++LNT  +N IS                              +G+ L+S+   GSR
Sbjct: 283077 KISEQILNT-PKNSISIG-------ISL*NLKID*NN*EEKN*ITKLGAILYSKKVMGSR 282922

Query: 421    VIDLKSNKKLLIGGLEFNNYGSHIDN 446
              VI  K NK  ++ G+EF NYGSH  N
Sbjct: 282921 VITNKKNK--VVCGIEFKNYGSHCGN 282850



 Score = 48.9 bits (115), Expect = 2e-05
 Identities = 23/58 (39%), Positives = 37/58 (63%)
 Frame = -1

Query: 421    VIDLKSNKKLLIGGLEFNNYGSHIDNYSTSYLTVACAIGITKLEIDTFIQRLSKLFNK 478
              V++ K  + +++  + +N     +DNY T Y+TVACAIG+TK EIDTF+    K + +
Sbjct: 282887 VLNSKITEVIVVTSISYNI----LDNYFTPYITVACAIGLTKQEIDTFV*EFEKAYTQ 282726


>scaffold_45
          Length = 476979

 Score = 30.4 bits (67), Expect = 6.8
 Identities = 24/60 (40%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
 Frame = +2

Query: 321    PILDVFITLLSMGKQGWLNLLKERKELLIY--FNEQLSKFALENNEKLLN-TINENKISF 377
              PI+  FI +L   + G +NL K   E +IY  F E    F   +     N TI ENKISF
Sbjct: 139832 PIIPPFIYILMFARIGIINL*KNLLEKIIYAKFEELNDNFNFVHFNS*SNLTIVENKISF 140011


>scaffold_13
          Length = 667686

 Score = 30.0 bits (66), Expect = 8.9
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = +1

Query: 216   GVDNILCVFSTTSCFAPRVPDKIIEISEICKRYNIGH 252
             G D  +  F+  SCF P  PD +  ISE+ +  N+ H
Sbjct: 54637 GFDFKMSDFTVRSCFRPLTPDDVAIISEVPEFKNVFH 54747


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,841,726
Number of Sequences: 697
Number of extensions: 527596
Number of successful extensions: 2001
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1881
Number of HSP's gapped (non-prelim): 323
length of query: 479
length of database: 23,960,378
effective HSP length: 110
effective length of query: 369
effective length of database: 23,883,708
effective search space: 8813088252
effective search space used: 8813088252
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)