TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium transferase [Dictyostelium discoideum AX4] (479 letters) Database: genome.fa 218 sequences; 41,633,360 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value scaffold_38 84 4e-28 >scaffold_38 Length = 375890 Score = 84.3 bits (207), Expect(2) = 4e-28 Identities = 54/172 (31%), Positives = 75/172 (43%), Gaps = 68/172 (39%) Frame = -2 Query: 241 ISEICKRYNIGHIINNAYGLQCSKILHNISQ----------------------------- 271 + +CK + H++NNAYGLQ +K +H I Sbjct: 202597 VGAVCKDLQVPHVVNNAYGLQSTKCIHLIETGG*FVPLWE*TLRTRNASTHARPFAPPDT 202418 Query: 272 ACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYP----------------- 314 A + GRVD +QSTDKN MVPVGG+I++ I +S+ YP Sbjct: 202417 AQRAGRVDFVVQSTDKNLMVPVGGSIVASSTPALITALSQTYPGQSQRILRGLG*PATCP 202238 Query: 315 ----------------------GRANSSPILDVFITLLSMGKQGWLNLLKER 344 GRA+S PI+DVF+TLLS+G+ G+ LL+ER Sbjct: 202237 LWS*SVLIVLVDPCPLLLASISGRASSGPIVDVFLTLLSLGQTGFKKLLEER 202082 Score = 62.4 bits (150), Expect(2) = 5e-19 Identities = 27/55 (49%), Positives = 44/55 (80%) Frame = -3 Query: 35 LLIHKKLPNIGYNDKIIELILNEISLMDSNNFIENIGVGEREGRIYSGLVEKRHY 89 LL+ + P G++D+ IEL+++E+++MDSNNF + G+GEREGRI +G+V +RH+ Sbjct: 203694 LLL*HQWPEQGWSDETIELLMHELAIMDSNNFPGSTGLGEREGRIAAGIVARRHF 203530 Score = 60.1 bits (144), Expect(2) = 4e-28 Identities = 32/77 (41%), Positives = 42/77 (54%) Frame = -3 Query: 161 NANNKRYVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMIRTDLVAIEDKIKELGVDNI 220 N K+ + W +C G PI+I + GD + TD+ AI I+E G D I Sbjct: 202920 NGQVKQRLTWFSSPSSAC------PGYTPIIIELRRSGDQLATDVTAIRQAIEEHGPDAI 202759 Query: 221 LCVFSTTSCFAPRVPDK 237 LCV +TTSCFAPR PD+ Sbjct: 202758 LCVVTTTSCFAPRSPDE 202708 Score = 51.2 bits (121), Expect(2) = 5e-19 Identities = 23/42 (54%), Positives = 29/42 (69%) Frame = -2 Query: 89 YGFAHGIGRSGDITEQQPKAAGSSLIQKLTHSLVLDAMKLAG 130 Y HGIGRSGDI QPKAAGSSL+ +LT + L +++ G Sbjct: 203443 YRLGHGIGRSGDIAAIQPKAAGSSLLARLTEKMTLQLLRVVG 203318 Score = 43.5 bits (101), Expect = 5e-04 Identities = 19/44 (43%), Positives = 31/44 (70%) Frame = -3 Query: 420 RVIDLKSNKKLLIGGLEFNNYGSHIDNYSTSYLTVACAIGITKL 463 RV+ + KK + GL+F YG+H+D+Y + Y+TVA AIG +++ Sbjct: 201576 RVVSGHAEKK--VAGLQFTGYGAHVDDYPSPYVTVAAAIGASEV 201451 Score = 39.3 bits (90), Expect = 0.009 Identities = 17/27 (62%), Positives = 23/27 (85%) Frame = -2 Query: 167 YVLWPRIDQKSCLKSIITAGLIPIVIP 193 YV+W RIDQK+CLK+I+TAG + +V P Sbjct: 202999 YVVWSRIDQKTCLKAIVTAGWL-LVFP 202922 Database: genome.fa Posted date: Jan 19, 2010 5:06 PM Number of letters in database: 41,633,360 Number of sequences in database: 218 Lambda K H 0.319 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 19,283,687 Number of Sequences: 218 Number of extensions: 254675 Number of successful extensions: 1132 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 1054 Number of HSP's gapped (non-prelim): 194 length of query: 479 length of database: 13,877,786 effective HSP length: 106 effective length of query: 373 effective length of database: 13,854,678 effective search space: 5167794894 effective search space used: 5167794894 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 64 (29.3 bits)