TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: genome.fa 
           1696 sequences; 31,706,682 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

mex400p23.q1k                                                         183   2e-46

>mex400p23.q1k 
          Length = 34044

 Score =  183 bits (465), Expect = 2e-46
 Identities = 124/378 (32%), Positives = 179/378 (47%), Gaps = 67/378 (17%)
 Frame = +2

Query: 166  RYVLWPRIDQKSCLKSIITAGLIPIVIP-------------------NQLDGDM------ 200
            RYV+WPRIDQK+ LK I  AGL+P+ +                    N LD D       
Sbjct: 6584 RYVIWPRIDQKTALKCIDAAGLVPVPVQLRPAVPLARSAAPCVSTNRNNLDRDQDSIASP 6763

Query: 201  ---IRTDLVAIEDKIKEL--------GVDNILCVFSTTSCFAPRVPDKIIEISEICKRYN 249
                 +  + +E  + ++        G   ++CV STTSCFAPR+PD  + +++ CK+  
Sbjct: 6764 STRTSSSSLFLECHVDDVAAAVSAVGGPSQVVCVLSTTSCFAPRLPDNTVALAQYCKKAG 6943

Query: 250  IGHIINNAYGLQCSKILHNISQACKLGRVDAFIQSTDKNFMVPVGGAIISGPNSEFIDQI 309
            I +++NNAYG+Q  +I+  +  A +LGRVD  +QS DKNF+VPVGG+II   + E    +
Sbjct: 6944 IPYVVNNAYGVQSRRIMTRLDAAQRLGRVDFVVQSGDKNFLVPVGGSIICSSDKERCKAV 7123

Query: 310  SRNYPGRANSSPILDVFITLLSMGKQGWLNLLKERKELLIYFNEQLSKFALENNEKLL-- 367
            +  Y GRA+ SPI+D+FIT LS+G++G   L ++R +       QL  FA E  E LL  
Sbjct: 7124 AALYAGRASMSPIVDLFITALSLGRRGMQALWRDRYKCRARLIRQLRVFARERREVLLVD 7303

Query: 368  ----------------NTIN----ENKISFAXXXXXXXXXXXXXXXXXXXXXXXXXXXM- 406
                            NT N     N IS A                           + 
Sbjct: 7304 DSDDDKDNEDTVGGSQNTSNAAVPRNDISVAVTMRAYGLPAAEASSSGAQLGSEQAEKVT 7483

Query: 407  -------IGSKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNY-STSYLTVACAI 458
                    G++LF  + +G RVI    +    I G +F NYG H D       L +AC I
Sbjct: 7484 NWAAARAFGAQLFRSAVTGPRVITPAPSTPTTIAGCKFRNYGMHQDREPPCPLLVIACGI 7663

Query: 459  GITKLEIDTFIQRLSKLF 476
            G+++ E+D  I RL  L+
Sbjct: 7664 GMSEAEVDALIARLRDLW 7717



 Score =  124 bits (310), Expect = 2e-28
 Identities = 59/130 (45%), Positives = 86/130 (66%)
 Frame = +2

Query: 1    MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEISL 60
            M+ + L+  +  +   YID   +      ++L ++L  +  P+ G  D  IELIL ++SL
Sbjct: 5945 MDDRALKLAEAFVSARYIDAGRESLQSTARILRSILAQRCCPDDGLTDAAIELILRQLSL 6124

Query: 61   MDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRSGDITEQQPKAAGSSLIQKLTHS 120
            MD+NNF  ++G GEREGR+ S LV  RH+G  HGIGRSGD+  +QPKAAGSSL+ K+T+ 
Sbjct: 6125 MDTNNFAHHVGGGEREGRVISSLVRMRHFGLTHGIGRSGDVLSEQPKAAGSSLLYKITNV 6304

Query: 121  LVLDAMKLAG 130
            L+LD ++ AG
Sbjct: 6305 LMLDLIRQAG 6334


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 31,706,682
  Number of sequences in database:  1696
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,553,163
Number of Sequences: 1696
Number of extensions: 166784
Number of successful extensions: 642
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 594
Number of HSP's gapped (non-prelim): 90
length of query: 479
length of database: 10,568,894
effective HSP length: 104
effective length of query: 375
effective length of database: 10,392,510
effective search space: 3897191250
effective search space used: 3897191250
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)