TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: genome.fa 
           2172 sequences; 20,771,095 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFB02000366.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    29   4.4  
gb|AAFB02000089.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   7.5  
gb|AAFB02000008.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   7.5  
gb|AAFB02000279.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   9.8  
gb|AAFB02000227.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   9.8  
gb|AAFB02000011.1| Entamoeba histolytica HM-1:IMSS gcontig_11047...    28   9.8  

>gb|AAFB02000366.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512360, whole genome
             shotgun sequence
          Length = 16111

 Score = 29.3 bits (64), Expect = 4.4
 Identities = 15/38 (39%), Positives = 23/38 (60%)
 Frame = -3

Query: 338   LNLLKERKELLIYFNEQLSKFALENNEKLLNTINENKI 375
             L LLK +KEL+ Y +  + KF +ENN  + N ++   I
Sbjct: 12773 LELLKFKKELIEYHSSIIEKF-IENNNSINNKVSSGTI 12663


>gb|AAFB02000089.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750504619, whole genome
             shotgun sequence
          Length = 46612

 Score = 28.5 bits (62), Expect = 7.5
 Identities = 18/56 (32%), Positives = 30/56 (53%)
 Frame = +2

Query: 4     KNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDKIIELILNEIS 59
             KN+ET   ++  +  D A    S    LL+ L I+ KLP++  + K + +I N +S
Sbjct: 29876 KNIETHDAVLYKTIYDNANNKFSNIINLLQKLFINIKLPSL-LDSKNVSIIKNIVS 30040


>gb|AAFB02000008.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512822, whole genome
             shotgun sequence
          Length = 118116

 Score = 28.5 bits (62), Expect = 7.5
 Identities = 22/56 (39%), Positives = 31/56 (55%), Gaps = 4/56 (7%)
 Frame = +3

Query: 306   IDQISRNYPGRANSSPILDVFITLLSMGKQG-WLN---LLKERKELLIYFNEQLSK 357
             IDQI++N+    + S  +  F  +L    QG WLN    +KER E L  FNE ++K
Sbjct: 59754 IDQINKNF----SKSLQIKKFGVVLEGTDQGMWLNEIKTIKERNEALKNFNEFITK 59909


>gb|AAFB02000279.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512132, whole genome
             shotgun sequence
          Length = 21389

 Score = 28.1 bits (61), Expect = 9.8
 Identities = 21/86 (24%), Positives = 38/86 (44%), Gaps = 2/86 (2%)
 Frame = -2

Query: 14    KGSYIDQAIQGTSQFNKLLETLLIHKKL--PNIGYNDKIIELILNEISLMDSNNFIENIG 71
             KGS     + G++     ++T+ IH  L     GY +K +E+ L+ I             
Sbjct: 15448 KGSIEKAQVHGSNVLYSPVQTVNIHCTLFLTKNGYEEKGLEVSLHSI------------- 15308

Query: 72    VGEREGRIYSGLVEKRHYGFAHGIGR 97
              G++   +  G V+  HY   +G+G+
Sbjct: 15307 -GKKGKELCKGYVDLAHYADLNGVGK 15233


>gb|AAFB02000227.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512904, whole genome
            shotgun sequence
          Length = 25440

 Score = 28.1 bits (61), Expect = 9.8
 Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 6/39 (15%)
 Frame = +1

Query: 217  VDNILCVFSTTSCFAPRVPDKI------IEISEICKRYN 249
            ++ ++C+F++  CF     +KI      I+IS IC  YN
Sbjct: 4219 INYVICIFNSKECFNRYYINKIFFSKYTIDISIICSHYN 4335


>gb|AAFB02000011.1| Entamoeba histolytica HM-1:IMSS gcontig_1104750512060, whole genome
             shotgun sequence
          Length = 107510

 Score = 28.1 bits (61), Expect = 9.8
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -2

Query: 59    SLMDSNNFIENIGVGEREGRIYSGL 83
             +L   NNFIE++    R+ +IY+GL
Sbjct: 51091 ALTKDNNFIEHMSKNNRDNKIYNGL 51017


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:04 PM
  Number of letters in database: 20,771,095
  Number of sequences in database:  2172
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,624,805
Number of Sequences: 2172
Number of extensions: 158771
Number of successful extensions: 743
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 47
Number of HSP's that attempted gapping in prelim test: 495
Number of HSP's gapped (non-prelim): 347
length of query: 479
length of database: 6,923,698
effective HSP length: 101
effective length of query: 378
effective length of database: 6,704,326
effective search space: 2534235228
effective search space used: 2534235228
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)