TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|66814240|ref|XP_641299.1| O-phosphoseryl-tRNA selenium
transferase [Dictyostelium discoideum AX4]
         (479 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_49                                                           624   e-179
scaffold_300                                                           30   4.1  

>scaffold_49 
          Length = 116333

 Score =  624 bits (1609), Expect = e-179
 Identities = 318/445 (71%), Positives = 353/445 (79%), Gaps = 5/445 (1%)
 Frame = +2

Query: 39    KKLPNIGYNDKIIELILNEISLMDSNNFIENIGVGEREGRIYSGLVEKRHYGFAHGIGRS 98
             ++LP IGY+DK+IELILNEIS+MDSNNFIENIGVGEREGRIYS LVEKRHYGF+HGIGRS
Sbjct: 59204 RRLPEIGYSDKVIELILNEISMMDSNNFIENIGVGEREGRIYSSLVEKRHYGFSHGIGRS 59383

Query: 99    GDITEQQPKAAGSSLIQKLTHSLVLDAMKLAGLEQSSLSNCXXXXXXXXXXXXXXXXXXK 158
             GDITEQQPKAAGSSLIQKL H LVLDAM++AGLE  + S C                  K
Sbjct: 59384 GDITEQQPKAAGSSLIQKLVHYLVLDAMRIAGLESQAASTCLLLPMATGMTLSLAMMTLK 59563

Query: 159   SINANNKRYVLWPRIDQKSCLKSIITAGLIPIVIPNQLDGDMIRTDLVAIEDKIKELGVD 218
               + +N RYV+WPRIDQKSCLKSI+TAGLIPIVI N+L+GDMIRTDL AI+ KI ELG  
Sbjct: 59564 QKSQSNARYVIWPRIDQKSCLKSIVTAGLIPIVIENKLEGDMIRTDLDAIKSKIIELGPS 59743

Query: 219   NILCVFSTTSCFAPRVPDKIIEISEICKRYNIGHIINNAYGLQCSKILHNISQACKLGRV 278
             NILCVFSTTSCFAPR PD+I+EIS++CK +NIGHIINNAYGLQCSKILH ISQ+CKLGRV
Sbjct: 59744 NILCVFSTTSCFAPRAPDRIVEISQLCKEHNIGHIINNAYGLQCSKILHAISQSCKLGRV 59923

Query: 279   DAFIQSTDKNFMVPVGGAIISGPNSEFIDQISRNYPGRANSSPILDVFITLLSMGKQGWL 338
             DA+IQSTDKNFMVPVGGAIISGPN  FI+ I++NYPGRANSSPILDVFITLLSMGK GW 
Sbjct: 59924 DAYIQSTDKNFMVPVGGAIISGPNKVFIEDIAKNYPGRANSSPILDVFITLLSMGKSGWS 60103

Query: 339   NLLKERKELLIYFNEQLSKFALENNEKLLNTINENKISFA-----XXXXXXXXXXXXXXX 393
              LLKERKELL+YF +QL K A E NEKLL+TINENKISFA                    
Sbjct: 60104 GLLKERKELLVYFQDQLGKLANEFNEKLLSTINENKISFAFSLKNFSSNKNEQQLESELN 60283

Query: 394   XXXXXXXXXXXXMIGSKLFSRSCSGSRVIDLKSNKKLLIGGLEFNNYGSHIDNYSTSYLT 453
                         M+GS+LFSRSCSG+RVIDL  NKK ++GGLEFN YGSHID Y TSYLT
Sbjct: 60284 NNNNNNNDYSPSMLGSQLFSRSCSGARVIDLSKNKKAIVGGLEFNYYGSHIDGYPTSYLT 60463

Query: 454   VACAIGITKLEIDTFIQRLSKLFNK 478
             VACAIG+TK EID FIQRLSKL  K
Sbjct: 60464 VACAIGVTKSEIDKFIQRLSKLLKK 60538



 Score = 67.8 bits (164), Expect = 2e-11
 Identities = 31/49 (63%), Positives = 37/49 (75%)
 Frame = +3

Query: 1     MNLKNLETCKGLIKGSYIDQAIQGTSQFNKLLETLLIHKKLPNIGYNDK 49
             MN KNLE CKGL+K +Y+DQAIQGTSQFNK++E+LLIH        N K
Sbjct: 58989 MNQKNLELCKGLVKSNYVDQAIQGTSQFNKIIESLLIHVNYSFTNINQK 59135


>scaffold_300 
          Length = 42091

 Score = 30.0 bits (66), Expect = 4.1
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +3

Query: 41   LPNIGYNDKIIELILNEISLMDSNNFIENIGVGEREGR 78
            L ++GYN+  +E ++N+     SNN     G   REGR
Sbjct: 3276 LADLGYNENSVEALINQYESRQSNN---RFGGNNREGR 3380


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.319    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,567,320
Number of Sequences: 799
Number of extensions: 245178
Number of successful extensions: 1124
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 60
Number of HSP's successfully gapped in prelim test: 37
Number of HSP's that attempted gapping in prelim test: 1020
Number of HSP's gapped (non-prelim): 236
length of query: 479
length of database: 10,989,169
effective HSP length: 104
effective length of query: 375
effective length of database: 10,906,073
effective search space: 4089777375
effective search space used: 4089777375
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)